Hydroxyurea-Induced miRNA Expression in Sickle Cell Disease Patients in Africa
Hydroxyurea (HU) is clinically beneficial in sickle cell disease (SCD) through fetal hemoglobin (HbF) induction; however, the mechanism of HU is not yet fully elucidated. Selected miRNAs have been associated with HU-induced HbF production. We have investigated differential HU-induced global miRNA ex...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.00509/full |
| _version_ | 1818027170687090688 |
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| author | Khuthala Mnika Gaston K. Mazandu Gaston K. Mazandu Mario Jonas Gift D. Pule Emile R. Chimusa Neil A. Hanchard Ambroise Wonkam |
| author_facet | Khuthala Mnika Gaston K. Mazandu Gaston K. Mazandu Mario Jonas Gift D. Pule Emile R. Chimusa Neil A. Hanchard Ambroise Wonkam |
| author_sort | Khuthala Mnika |
| collection | DOAJ |
| description | Hydroxyurea (HU) is clinically beneficial in sickle cell disease (SCD) through fetal hemoglobin (HbF) induction; however, the mechanism of HU is not yet fully elucidated. Selected miRNAs have been associated with HU-induced HbF production. We have investigated differential HU-induced global miRNA expression in peripheral blood of adult SCD patients in patients from Congo, living in South Africa. We found 22 of 798 miRNAs evaluated that were differentially expressed under HU treatment, with the majority (13/22) being functionally associated with HbF-regulatory genes, including BCL11A (miR-148b-3p, miR-32-5p, miR-340-5p, and miR-29c-3p), MYB (miR-105-5p), and KLF-3 (miR-106b-5), and SP1 (miR-29b-3p, miR-625-5p, miR-324-5p, miR-125a-5p, miR-99b-5p, miR-374b-5p, and miR-145-5p). The preliminary study provides potential additional miRNA candidates for therapeutic exploration. |
| first_indexed | 2024-12-10T04:43:39Z |
| format | Article |
| id | doaj.art-f5661fb2d8f74c3b924a34b60cad6ba1 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-10T04:43:39Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-f5661fb2d8f74c3b924a34b60cad6ba12022-12-22T02:01:49ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-05-011010.3389/fgene.2019.00509437181Hydroxyurea-Induced miRNA Expression in Sickle Cell Disease Patients in AfricaKhuthala Mnika0Gaston K. Mazandu1Gaston K. Mazandu2Mario Jonas3Gift D. Pule4Emile R. Chimusa5Neil A. Hanchard6Ambroise Wonkam7Division of Human Genetics, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South AfricaDivision of Human Genetics, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South AfricaAfrican Institute for Mathematical Sciences, Cape Town, South AfricaDivision of Human Genetics, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South AfricaDivision of Human Genetics, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South AfricaDivision of Human Genetics, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South AfricaDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, United StatesDivision of Human Genetics, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South AfricaHydroxyurea (HU) is clinically beneficial in sickle cell disease (SCD) through fetal hemoglobin (HbF) induction; however, the mechanism of HU is not yet fully elucidated. Selected miRNAs have been associated with HU-induced HbF production. We have investigated differential HU-induced global miRNA expression in peripheral blood of adult SCD patients in patients from Congo, living in South Africa. We found 22 of 798 miRNAs evaluated that were differentially expressed under HU treatment, with the majority (13/22) being functionally associated with HbF-regulatory genes, including BCL11A (miR-148b-3p, miR-32-5p, miR-340-5p, and miR-29c-3p), MYB (miR-105-5p), and KLF-3 (miR-106b-5), and SP1 (miR-29b-3p, miR-625-5p, miR-324-5p, miR-125a-5p, miR-99b-5p, miR-374b-5p, and miR-145-5p). The preliminary study provides potential additional miRNA candidates for therapeutic exploration.https://www.frontiersin.org/article/10.3389/fgene.2019.00509/fullsickle cell diseasefetal hemoglobinhydroxyureamiRNAAfrica |
| spellingShingle | Khuthala Mnika Gaston K. Mazandu Gaston K. Mazandu Mario Jonas Gift D. Pule Emile R. Chimusa Neil A. Hanchard Ambroise Wonkam Hydroxyurea-Induced miRNA Expression in Sickle Cell Disease Patients in Africa Frontiers in Genetics sickle cell disease fetal hemoglobin hydroxyurea miRNA Africa |
| title | Hydroxyurea-Induced miRNA Expression in Sickle Cell Disease Patients in Africa |
| title_full | Hydroxyurea-Induced miRNA Expression in Sickle Cell Disease Patients in Africa |
| title_fullStr | Hydroxyurea-Induced miRNA Expression in Sickle Cell Disease Patients in Africa |
| title_full_unstemmed | Hydroxyurea-Induced miRNA Expression in Sickle Cell Disease Patients in Africa |
| title_short | Hydroxyurea-Induced miRNA Expression in Sickle Cell Disease Patients in Africa |
| title_sort | hydroxyurea induced mirna expression in sickle cell disease patients in africa |
| topic | sickle cell disease fetal hemoglobin hydroxyurea miRNA Africa |
| url | https://www.frontiersin.org/article/10.3389/fgene.2019.00509/full |
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