Influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery: a prospective, blinded, controlled observational study
Objective: Intraoperative bacterial contamination is a risk factor for surgical site infections (SSIs). This prospective, randomized, blinded, controlled trial (Reg. No. BB08/12) investigated the effect of a cyanoacrylate-based skin sealant (InteguSeal) on intraoperative wound contamination during t...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-12-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971214016233 |
| _version_ | 1818808042159865856 |
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| author | Georg Daeschlein Matthias Napp Ojan Assadian Jessica Bluhm Colin Krueger Sebastian von Podewils Denis Gümbel Peter Hinz Hermann Haase Pascal M. Dohmen Torsten Koburger Axel Ekkernkamp Axel Kramer |
| author_facet | Georg Daeschlein Matthias Napp Ojan Assadian Jessica Bluhm Colin Krueger Sebastian von Podewils Denis Gümbel Peter Hinz Hermann Haase Pascal M. Dohmen Torsten Koburger Axel Ekkernkamp Axel Kramer |
| author_sort | Georg Daeschlein |
| collection | DOAJ |
| description | Objective: Intraoperative bacterial contamination is a risk factor for surgical site infections (SSIs). This prospective, randomized, blinded, controlled trial (Reg. No. BB08/12) investigated the effect of a cyanoacrylate-based skin sealant (InteguSeal) on intraoperative wound contamination during trauma surgery.
Methods: A total of 128 patients undergoing trauma surgery were assigned randomly to an intervention (n = 62) or a control group (n = 66). Surgical sites were investigated at three locations: maximum incision depth (base), wound margin prior to wound closure (margin), and the surgical sutures (suture). Colony-forming units (CFU) were counted after 48 h of incubation.
Results: Overall, significantly lower CFU counts were obtained for samples from the intervention group at all three sample sites compared to the control group. The difference, however, was only significant for the suture site (p = 0.040).
Conclusions: Preoperative sealing reduced microbial contamination on sutures during surgery, while the overall wound contamination remained unchanged. Hence, prevention of the clinically more relevant deep SSIs may not be expected. However, this study was not designed to detect differences in the rate of SSI. The role of the reduction in suture contamination with regard to the prevention of SSI remains to be evaluated. |
| first_indexed | 2024-12-18T19:35:16Z |
| format | Article |
| id | doaj.art-f567dd98a4e14c209d0dd1dc7a5ea361 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-12-18T19:35:16Z |
| publishDate | 2014-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-f567dd98a4e14c209d0dd1dc7a5ea3612022-12-21T20:55:37ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112014-12-0129C27427810.1016/j.ijid.2014.08.008Influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery: a prospective, blinded, controlled observational studyGeorg Daeschlein0Matthias Napp1Ojan Assadian2Jessica Bluhm3Colin Krueger4Sebastian von Podewils5Denis Gümbel6Peter Hinz7Hermann Haase8Pascal M. Dohmen9Torsten Koburger10Axel Ekkernkamp11Axel Kramer12Department of Dermatology, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyDepartment of Trauma and Orthopaedic Surgery, Clinic of Surgery, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyDepartment for Hospital Hygiene and Infection Control, Medical University of Vienna, AustriaInstitute of Hygiene and Environmental Medicine, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyDepartment for General, Visceral and Vascular Surgery, Vivantes Humboldt-Klinikum, Berlin, GermanyDepartment of Dermatology, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyDepartment of Trauma and Orthopaedic Surgery, Clinic of Surgery, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyDepartment of Trauma and Orthopaedic Surgery, Clinic of Surgery, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyDepartment of Dermatology, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyHeart Centre Leipzig GmbH, University Clinic Leipzig, Leipzig, GermanyHygiene Nord GmbH, Greifswald, GermanyDepartment of Trauma and Orthopaedic Surgery, Clinic of Surgery, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyInstitute of Hygiene and Environmental Medicine, University Medicine, Ernst-Moritz-Arndt University Greifswald, GermanyObjective: Intraoperative bacterial contamination is a risk factor for surgical site infections (SSIs). This prospective, randomized, blinded, controlled trial (Reg. No. BB08/12) investigated the effect of a cyanoacrylate-based skin sealant (InteguSeal) on intraoperative wound contamination during trauma surgery. Methods: A total of 128 patients undergoing trauma surgery were assigned randomly to an intervention (n = 62) or a control group (n = 66). Surgical sites were investigated at three locations: maximum incision depth (base), wound margin prior to wound closure (margin), and the surgical sutures (suture). Colony-forming units (CFU) were counted after 48 h of incubation. Results: Overall, significantly lower CFU counts were obtained for samples from the intervention group at all three sample sites compared to the control group. The difference, however, was only significant for the suture site (p = 0.040). Conclusions: Preoperative sealing reduced microbial contamination on sutures during surgery, while the overall wound contamination remained unchanged. Hence, prevention of the clinically more relevant deep SSIs may not be expected. However, this study was not designed to detect differences in the rate of SSI. The role of the reduction in suture contamination with regard to the prevention of SSI remains to be evaluated.http://www.sciencedirect.com/science/article/pii/S1201971214016233Microbial sealantCyanoacrylateInteguSealWoundSSIPrevention |
| spellingShingle | Georg Daeschlein Matthias Napp Ojan Assadian Jessica Bluhm Colin Krueger Sebastian von Podewils Denis Gümbel Peter Hinz Hermann Haase Pascal M. Dohmen Torsten Koburger Axel Ekkernkamp Axel Kramer Influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery: a prospective, blinded, controlled observational study International Journal of Infectious Diseases Microbial sealant Cyanoacrylate InteguSeal Wound SSI Prevention |
| title | Influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery: a prospective, blinded, controlled observational study |
| title_full | Influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery: a prospective, blinded, controlled observational study |
| title_fullStr | Influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery: a prospective, blinded, controlled observational study |
| title_full_unstemmed | Influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery: a prospective, blinded, controlled observational study |
| title_short | Influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery: a prospective, blinded, controlled observational study |
| title_sort | influence of preoperative skin sealing with cyanoacrylate on microbial contamination of surgical wounds following trauma surgery a prospective blinded controlled observational study |
| topic | Microbial sealant Cyanoacrylate InteguSeal Wound SSI Prevention |
| url | http://www.sciencedirect.com/science/article/pii/S1201971214016233 |
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