Self-Entrapment of Antimicrobial Peptides in Silica Particles for Stable and Effective Antimicrobial Peptide Delivery System
Antimicrobial peptides (AMPs) have emerged as a promising solution to tackle bacterial infections and combat antibiotic resistance. However, their vulnerability to protease degradation and toxicity towards mammalian cells has hindered their clinical application. To overcome these challenges, our stu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16423 |
| _version_ | 1797459021789134848 |
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| author | Mi-Ran Ki Sung Ho Kim Tae In Park Seung Pil Pack |
| author_facet | Mi-Ran Ki Sung Ho Kim Tae In Park Seung Pil Pack |
| author_sort | Mi-Ran Ki |
| collection | DOAJ |
| description | Antimicrobial peptides (AMPs) have emerged as a promising solution to tackle bacterial infections and combat antibiotic resistance. However, their vulnerability to protease degradation and toxicity towards mammalian cells has hindered their clinical application. To overcome these challenges, our study aims to develop a method to enhance the stability and safety of AMPs applicable to effective drug–device combination products. The KR12 antimicrobial peptide was chosen, and in order to further enhance its delivery and efficacy the human immunodeficiency virus TAT protein-derived cell-penetrating peptide (CPP) was fused to form CPP-KR12. A new product, CPP-KR12@Si, was developed by forming silica particles with self-entrapped CPP-KR12 peptide using biomimetic silica precipitability because of its cationic nature. Peptide delivery from CPP-KR12@Si to bacteria and cells was observed at a slightly delivered rate, with improved stability against trypsin treatment and a reduction in cytotoxicity compared to CPP-KR12. Finally, the antimicrobial potential of the CPP-KR12@Si/bone graft substitute (BGS) combination product was demonstrated. CPP-KR12 is coated in the form of submicron-sized particles on the surface of the BGS. Self-entrapped AMP in silica nanoparticles is a safe and effective AMP delivery method that will be useful for developing a drug–device combination product for tissue regeneration. |
| first_indexed | 2024-03-09T16:45:31Z |
| format | Article |
| id | doaj.art-f56ab03b46b0439d82c8900c34dc49ac |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:45:31Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f56ab03b46b0439d82c8900c34dc49ac2023-11-24T14:47:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221642310.3390/ijms242216423Self-Entrapment of Antimicrobial Peptides in Silica Particles for Stable and Effective Antimicrobial Peptide Delivery SystemMi-Ran Ki0Sung Ho Kim1Tae In Park2Seung Pil Pack3Department of Biotechnology and Bioinformatics, Korea University, Sejong-ro 2511, Sejong 30019, Republic of KoreaDepartment of Biotechnology and Bioinformatics, Korea University, Sejong-ro 2511, Sejong 30019, Republic of KoreaDepartment of Biotechnology and Bioinformatics, Korea University, Sejong-ro 2511, Sejong 30019, Republic of KoreaDepartment of Biotechnology and Bioinformatics, Korea University, Sejong-ro 2511, Sejong 30019, Republic of KoreaAntimicrobial peptides (AMPs) have emerged as a promising solution to tackle bacterial infections and combat antibiotic resistance. However, their vulnerability to protease degradation and toxicity towards mammalian cells has hindered their clinical application. To overcome these challenges, our study aims to develop a method to enhance the stability and safety of AMPs applicable to effective drug–device combination products. The KR12 antimicrobial peptide was chosen, and in order to further enhance its delivery and efficacy the human immunodeficiency virus TAT protein-derived cell-penetrating peptide (CPP) was fused to form CPP-KR12. A new product, CPP-KR12@Si, was developed by forming silica particles with self-entrapped CPP-KR12 peptide using biomimetic silica precipitability because of its cationic nature. Peptide delivery from CPP-KR12@Si to bacteria and cells was observed at a slightly delivered rate, with improved stability against trypsin treatment and a reduction in cytotoxicity compared to CPP-KR12. Finally, the antimicrobial potential of the CPP-KR12@Si/bone graft substitute (BGS) combination product was demonstrated. CPP-KR12 is coated in the form of submicron-sized particles on the surface of the BGS. Self-entrapped AMP in silica nanoparticles is a safe and effective AMP delivery method that will be useful for developing a drug–device combination product for tissue regeneration.https://www.mdpi.com/1422-0067/24/22/16423antimicrobial peptidecell penetrating peptidesilica forming peptidebiomimetic silica depositiondrug deliverydrug device combination |
| spellingShingle | Mi-Ran Ki Sung Ho Kim Tae In Park Seung Pil Pack Self-Entrapment of Antimicrobial Peptides in Silica Particles for Stable and Effective Antimicrobial Peptide Delivery System International Journal of Molecular Sciences antimicrobial peptide cell penetrating peptide silica forming peptide biomimetic silica deposition drug delivery drug device combination |
| title | Self-Entrapment of Antimicrobial Peptides in Silica Particles for Stable and Effective Antimicrobial Peptide Delivery System |
| title_full | Self-Entrapment of Antimicrobial Peptides in Silica Particles for Stable and Effective Antimicrobial Peptide Delivery System |
| title_fullStr | Self-Entrapment of Antimicrobial Peptides in Silica Particles for Stable and Effective Antimicrobial Peptide Delivery System |
| title_full_unstemmed | Self-Entrapment of Antimicrobial Peptides in Silica Particles for Stable and Effective Antimicrobial Peptide Delivery System |
| title_short | Self-Entrapment of Antimicrobial Peptides in Silica Particles for Stable and Effective Antimicrobial Peptide Delivery System |
| title_sort | self entrapment of antimicrobial peptides in silica particles for stable and effective antimicrobial peptide delivery system |
| topic | antimicrobial peptide cell penetrating peptide silica forming peptide biomimetic silica deposition drug delivery drug device combination |
| url | https://www.mdpi.com/1422-0067/24/22/16423 |
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