By-product formation in repetitive PCR amplification of DNA libraries during SELEX.
The selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that prohibits the enrichment of target-recognizing...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4260880?pdf=render |
| _version_ | 1828399140298555392 |
|---|---|
| author | Fabian Tolle Julian Wilke Jesper Wengel Günter Mayer |
| author_facet | Fabian Tolle Julian Wilke Jesper Wengel Günter Mayer |
| author_sort | Fabian Tolle |
| collection | DOAJ |
| description | The selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that prohibits the enrichment of target-recognizing aptamers. Little is known about the formation of such by-products when employing nucleic acid libraries as templates. We report on the formation of two different forms of by-products, named ladder- and non-ladder-type observed during repetitive amplification in the course of in vitro selection experiments. Based on sequence information and the amplification behaviour of defined enriched nucleic acid molecules we suppose a molecular mechanism through which these amplification by-products are built. Better understanding of these mechanisms might help to find solutions minimizing by-product formation and improving the success rate of aptamer selection. |
| first_indexed | 2024-12-10T09:12:07Z |
| format | Article |
| id | doaj.art-f56aeec21d5d487a802dc24ee679231b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T09:12:07Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f56aeec21d5d487a802dc24ee679231b2022-12-22T01:54:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11469310.1371/journal.pone.0114693By-product formation in repetitive PCR amplification of DNA libraries during SELEX.Fabian TolleJulian WilkeJesper WengelGünter MayerThe selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that prohibits the enrichment of target-recognizing aptamers. Little is known about the formation of such by-products when employing nucleic acid libraries as templates. We report on the formation of two different forms of by-products, named ladder- and non-ladder-type observed during repetitive amplification in the course of in vitro selection experiments. Based on sequence information and the amplification behaviour of defined enriched nucleic acid molecules we suppose a molecular mechanism through which these amplification by-products are built. Better understanding of these mechanisms might help to find solutions minimizing by-product formation and improving the success rate of aptamer selection.http://europepmc.org/articles/PMC4260880?pdf=render |
| spellingShingle | Fabian Tolle Julian Wilke Jesper Wengel Günter Mayer By-product formation in repetitive PCR amplification of DNA libraries during SELEX. PLoS ONE |
| title | By-product formation in repetitive PCR amplification of DNA libraries during SELEX. |
| title_full | By-product formation in repetitive PCR amplification of DNA libraries during SELEX. |
| title_fullStr | By-product formation in repetitive PCR amplification of DNA libraries during SELEX. |
| title_full_unstemmed | By-product formation in repetitive PCR amplification of DNA libraries during SELEX. |
| title_short | By-product formation in repetitive PCR amplification of DNA libraries during SELEX. |
| title_sort | by product formation in repetitive pcr amplification of dna libraries during selex |
| url | http://europepmc.org/articles/PMC4260880?pdf=render |
| work_keys_str_mv | AT fabiantolle byproductformationinrepetitivepcramplificationofdnalibrariesduringselex AT julianwilke byproductformationinrepetitivepcramplificationofdnalibrariesduringselex AT jesperwengel byproductformationinrepetitivepcramplificationofdnalibrariesduringselex AT guntermayer byproductformationinrepetitivepcramplificationofdnalibrariesduringselex |