Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication
Halal medicine is an interesting topic to always discuss because it is a priority choice for Muslim consumers, one of which is halal capsules. Currently, molecular biology techniques such as real-time polymerase chain reactions are rapidly developing, including for the analysis of non-halal componen...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Ahmad Dahlan
2023-03-01
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| Series: | Pharmaciana |
| Subjects: | |
| Online Access: | http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/25694/pdf_258 |
| _version_ | 1797838011647393792 |
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| author | Nina Salamah Any Guntarti Laela Hayu Nurani |
| author_facet | Nina Salamah Any Guntarti Laela Hayu Nurani |
| author_sort | Nina Salamah |
| collection | DOAJ |
| description | Halal medicine is an interesting topic to always discuss because it is a priority choice for Muslim consumers, one of which is halal capsules. Currently, molecular biology techniques such as real-time polymerase chain reactions are rapidly developing, including for the analysis of non-halal components based on DNA sequences. This study aimed to validate the quantitative PCR method for identifying DNA in gelatin-based products and to apply the confirmation method designed for capsule samples on the market circulating in Yogyakarta to prove the halalness of these samples. Validation of the porcine DNA detection analysis method on standard extraction of porcine gelatin using primer pairs obtained in previous studies. Validated methods are used for testing market capsule shells. The qPCR method using D-loop primers is specifically capable of amplifying porcine gelatin DNA up to a concentration of 0.5 pg/µL, with a CV value in the amplification response of porcine gelatin DNA isolates (1000 pg/µL) of 0.85% which meets the test criteria using the PCR. Three samples of commercial soft capsules tested gave a positive amplification response, meaning that the samples tested contained porcine DNA, and one negative sample, which probably had non-porcine gelatin. The application of this method is also very useful for ensuring the authenticity of the capsule shell, especially from cross-contamination and counterfeiting. |
| first_indexed | 2024-04-09T15:35:01Z |
| format | Article |
| id | doaj.art-f56c228b8cbc4fc287596ecaef160fbb |
| institution | Directory Open Access Journal |
| issn | 2088-4559 2477-0256 |
| language | English |
| last_indexed | 2024-04-09T15:35:01Z |
| publishDate | 2023-03-01 |
| publisher | Universitas Ahmad Dahlan |
| record_format | Article |
| series | Pharmaciana |
| spelling | doaj.art-f56c228b8cbc4fc287596ecaef160fbb2023-04-28T02:02:50ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562023-03-01131162410.12928/pharmaciana.v13i1.25694Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthicationNina Salamah0Any Guntarti1Laela Hayu Nurani2Faculty of Pharmacy, Universitas Ahmad DahlanFaculty of Pharmacy, Universitas Ahmad DahlanFaculty of Pharmacy, Universitas Ahmad DahlanHalal medicine is an interesting topic to always discuss because it is a priority choice for Muslim consumers, one of which is halal capsules. Currently, molecular biology techniques such as real-time polymerase chain reactions are rapidly developing, including for the analysis of non-halal components based on DNA sequences. This study aimed to validate the quantitative PCR method for identifying DNA in gelatin-based products and to apply the confirmation method designed for capsule samples on the market circulating in Yogyakarta to prove the halalness of these samples. Validation of the porcine DNA detection analysis method on standard extraction of porcine gelatin using primer pairs obtained in previous studies. Validated methods are used for testing market capsule shells. The qPCR method using D-loop primers is specifically capable of amplifying porcine gelatin DNA up to a concentration of 0.5 pg/µL, with a CV value in the amplification response of porcine gelatin DNA isolates (1000 pg/µL) of 0.85% which meets the test criteria using the PCR. Three samples of commercial soft capsules tested gave a positive amplification response, meaning that the samples tested contained porcine DNA, and one negative sample, which probably had non-porcine gelatin. The application of this method is also very useful for ensuring the authenticity of the capsule shell, especially from cross-contamination and counterfeiting.http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/25694/pdf_258dnahalalporcine gelatinreal-time pcrsoft capsule |
| spellingShingle | Nina Salamah Any Guntarti Laela Hayu Nurani Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication Pharmaciana dna halal porcine gelatin real-time pcr soft capsule |
| title | Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication |
| title_full | Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication |
| title_fullStr | Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication |
| title_full_unstemmed | Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication |
| title_short | Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication |
| title_sort | analysis of soft gelatin capsule with real time polymerase chain reaction for halal autenthication |
| topic | dna halal porcine gelatin real-time pcr soft capsule |
| url | http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/25694/pdf_258 |
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