A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate
This paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-l-ascorbic acid trisodium salt, used a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-01-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/15/2/2232 |
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| author | Ana Lorena Alvarado-Gámez María Asunción Alonso-Lomillo Olga Domínguez-Renedo María Julia Arcos-Martínez |
| author_facet | Ana Lorena Alvarado-Gámez María Asunción Alonso-Lomillo Olga Domínguez-Renedo María Julia Arcos-Martínez |
| author_sort | Ana Lorena Alvarado-Gámez |
| collection | DOAJ |
| description | This paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-l-ascorbic acid trisodium salt, used as substrate, was affected by tungsten ions, which resulted in a decrease of chronoamperometric current, when a potential of 200 mV was applied on 10 mM of substrate in a Tris HCl buffer pH 8.00 and 0.36 M of KCl. Calibration curves for the electrochemical method validation, give a reproducibility of 5.2% (n = 3), a repeatability of 9.4% (n = 3) and a detection limit of 0.29 ± 0.01 µM. Enriched tap water, purified laboratory water and bottled drinking water, with a certified tungsten reference solution traceable to NIST, gave a recovery of 97.1%, 99.1% and 99.1% respectively (n = 4 in each case) and a dynamic range from 0.6 to 30 µM. This study was performed by means of a Lineweaver–Burk plot, showing a mixed kinetic inhibition. |
| first_indexed | 2024-04-11T11:03:37Z |
| format | Article |
| id | doaj.art-f577095c965343cfb61274877f20c883 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T11:03:37Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-f577095c965343cfb61274877f20c8832022-12-22T04:28:29ZengMDPI AGSensors1424-82202015-01-011522232224310.3390/s150202232s150202232A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a SubstrateAna Lorena Alvarado-Gámez0María Asunción Alonso-Lomillo1Olga Domínguez-Renedo2María Julia Arcos-Martínez3School of Chemistry & CELEQ, University of Costa Rica, San Pedro de Montes de Oca, 11500-2060 San José, Costa RicaDepartment of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, SpainDepartment of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, SpainDepartment of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, SpainThis paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-l-ascorbic acid trisodium salt, used as substrate, was affected by tungsten ions, which resulted in a decrease of chronoamperometric current, when a potential of 200 mV was applied on 10 mM of substrate in a Tris HCl buffer pH 8.00 and 0.36 M of KCl. Calibration curves for the electrochemical method validation, give a reproducibility of 5.2% (n = 3), a repeatability of 9.4% (n = 3) and a detection limit of 0.29 ± 0.01 µM. Enriched tap water, purified laboratory water and bottled drinking water, with a certified tungsten reference solution traceable to NIST, gave a recovery of 97.1%, 99.1% and 99.1% respectively (n = 4 in each case) and a dynamic range from 0.6 to 30 µM. This study was performed by means of a Lineweaver–Burk plot, showing a mixed kinetic inhibition.http://www.mdpi.com/1424-8220/15/2/2232alkaline phosphatase chronoamperometry2-phospho-l-ascorbic acid trisodium saltscreen printed carbon electrode tungsten |
| spellingShingle | Ana Lorena Alvarado-Gámez María Asunción Alonso-Lomillo Olga Domínguez-Renedo María Julia Arcos-Martínez A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate Sensors alkaline phosphatase chronoamperometry 2-phospho-l-ascorbic acid trisodium salt screen printed carbon electrode tungsten |
| title | A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate |
| title_full | A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate |
| title_fullStr | A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate |
| title_full_unstemmed | A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate |
| title_short | A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate |
| title_sort | chronoamperometric screen printed carbon biosensor based on alkaline phosphatase inhibition for w vi determination in water using 2 phospho l ascorbic acid trisodium salt as a substrate |
| topic | alkaline phosphatase chronoamperometry 2-phospho-l-ascorbic acid trisodium salt screen printed carbon electrode tungsten |
| url | http://www.mdpi.com/1424-8220/15/2/2232 |
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