Effect of Oligogalacturonides on Seed Germination and Disease Resistance of Sugar Beet Seedling and Root
Oligogalacturonides (OGs) are a bioactive carbohydrate derived from homogalacturonan. The OGs synthesized in this study significantly inhibited the mycelial growth of <i>Rhizoctonia solani</i> AG-4HGI in vitro, even at a low concentration (10 mg/L). The seed vigor test demonstrated that...
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MDPI AG
2022-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/7/716 |
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| author | Can Zhao Chunyan Wu Kuikui Li John F. Kennedy Michael Wisniewski Lihong Gao Chenggui Han Jia Liu Heng Yin Xuehong Wu |
| author_facet | Can Zhao Chunyan Wu Kuikui Li John F. Kennedy Michael Wisniewski Lihong Gao Chenggui Han Jia Liu Heng Yin Xuehong Wu |
| author_sort | Can Zhao |
| collection | DOAJ |
| description | Oligogalacturonides (OGs) are a bioactive carbohydrate derived from homogalacturonan. The OGs synthesized in this study significantly inhibited the mycelial growth of <i>Rhizoctonia solani</i> AG-4HGI in vitro, even at a low concentration (10 mg/L). The seed vigor test demonstrated that the application of 50 mg/L OGs to sugar beet seeds significantly increased average germination percentage, germination energy, germination index, and seedling vigor index. The same concentration of OGs also improved the seedling emergence percentage of sugar beet when seeds were sown in soil inoculated with D2 and D31 isolates, respectively. The lesion diameter on mature sugar beet roots caused by <i>R. solani</i> AG-4HGI isolates D2 and D31 also decreased by 40.60% and 39.86%, respectively, in sugar beets roots first treated with 50 mg/mL OGs in the wound site, relative to lesion size in untreated/pathogen inoculated wounds. Sugar beet roots treated with 50 mg/mL OGs prior to inoculation with the D2 isolate exhibited up-regulation of the defense-related genes glutathione peroxidase (GPX) and superoxide dismutase (SOD) by 2.4- and 1.6-fold, respectively, relative to control roots. Sugar beet roots treated with 50 mg/mL OGs prior to inoculation with D31 exhibited a 2.0- and 1.6-fold up-regulation of GPX and SOD, respectively, relative to the control. Our results indicate that OGs have the potential to be used for the protection of sugar beet against <i>R. solani</i> AG-4HGI. |
| first_indexed | 2024-03-09T10:16:53Z |
| format | Article |
| id | doaj.art-f5772dbfbd284611ba5aedbce9253039 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T10:16:53Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-f5772dbfbd284611ba5aedbce92530392023-12-01T22:20:04ZengMDPI AGJournal of Fungi2309-608X2022-07-018771610.3390/jof8070716Effect of Oligogalacturonides on Seed Germination and Disease Resistance of Sugar Beet Seedling and RootCan Zhao0Chunyan Wu1Kuikui Li2John F. Kennedy3Michael Wisniewski4Lihong Gao5Chenggui Han6Jia Liu7Heng Yin8Xuehong Wu9College of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaDalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaChembiotech Laboratories, Kyrewood House Tenbury Wells, Worcestershire WR15 8SG, UKDepartment of Biological Sciences, Virginia Polytechnic Institute and State University, 220 Ag Quad Ln, Blacksburg, VA 24061, USACollege of Horticulture, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaChongqing Key Laboratory of Economic Plant Biotechnology, College of Landscape Architecture and Life Science/Institute of Special Plants, Chongqing University of Arts and Sciences, Chongqing 402160, ChinaDalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaOligogalacturonides (OGs) are a bioactive carbohydrate derived from homogalacturonan. The OGs synthesized in this study significantly inhibited the mycelial growth of <i>Rhizoctonia solani</i> AG-4HGI in vitro, even at a low concentration (10 mg/L). The seed vigor test demonstrated that the application of 50 mg/L OGs to sugar beet seeds significantly increased average germination percentage, germination energy, germination index, and seedling vigor index. The same concentration of OGs also improved the seedling emergence percentage of sugar beet when seeds were sown in soil inoculated with D2 and D31 isolates, respectively. The lesion diameter on mature sugar beet roots caused by <i>R. solani</i> AG-4HGI isolates D2 and D31 also decreased by 40.60% and 39.86%, respectively, in sugar beets roots first treated with 50 mg/mL OGs in the wound site, relative to lesion size in untreated/pathogen inoculated wounds. Sugar beet roots treated with 50 mg/mL OGs prior to inoculation with the D2 isolate exhibited up-regulation of the defense-related genes glutathione peroxidase (GPX) and superoxide dismutase (SOD) by 2.4- and 1.6-fold, respectively, relative to control roots. Sugar beet roots treated with 50 mg/mL OGs prior to inoculation with D31 exhibited a 2.0- and 1.6-fold up-regulation of GPX and SOD, respectively, relative to the control. Our results indicate that OGs have the potential to be used for the protection of sugar beet against <i>R. solani</i> AG-4HGI.https://www.mdpi.com/2309-608X/8/7/716antioxidant responseoligogalactouronide preparationdamage-associated molecular patternsseedling damping-offroot and crown rotsugar beet |
| spellingShingle | Can Zhao Chunyan Wu Kuikui Li John F. Kennedy Michael Wisniewski Lihong Gao Chenggui Han Jia Liu Heng Yin Xuehong Wu Effect of Oligogalacturonides on Seed Germination and Disease Resistance of Sugar Beet Seedling and Root Journal of Fungi antioxidant response oligogalactouronide preparation damage-associated molecular patterns seedling damping-off root and crown rot sugar beet |
| title | Effect of Oligogalacturonides on Seed Germination and Disease Resistance of Sugar Beet Seedling and Root |
| title_full | Effect of Oligogalacturonides on Seed Germination and Disease Resistance of Sugar Beet Seedling and Root |
| title_fullStr | Effect of Oligogalacturonides on Seed Germination and Disease Resistance of Sugar Beet Seedling and Root |
| title_full_unstemmed | Effect of Oligogalacturonides on Seed Germination and Disease Resistance of Sugar Beet Seedling and Root |
| title_short | Effect of Oligogalacturonides on Seed Germination and Disease Resistance of Sugar Beet Seedling and Root |
| title_sort | effect of oligogalacturonides on seed germination and disease resistance of sugar beet seedling and root |
| topic | antioxidant response oligogalactouronide preparation damage-associated molecular patterns seedling damping-off root and crown rot sugar beet |
| url | https://www.mdpi.com/2309-608X/8/7/716 |
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