Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population
Dendritic cells residing in the skin represent a large family of antigen-presenting cells, ranging from long-lived Langerhans cells (LC) in the epidermis to various distinct classical dendritic cell subsets in the dermis. Through genetic fate mapping analysis and single-cell RNA-sequencing, we have...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/65412 |
| _version_ | 1828380009029435392 |
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| author | Jianpeng Sheng Qi Chen Xiaoting Wu Yu Wen Dong Johannes Mayer Junlei Zhang Lin Wang Xueli Bai Tingbo Liang Yang Ho Sung Wilson Wen Bin Goh Franca Ronchese Christiane Ruedl |
| author_facet | Jianpeng Sheng Qi Chen Xiaoting Wu Yu Wen Dong Johannes Mayer Junlei Zhang Lin Wang Xueli Bai Tingbo Liang Yang Ho Sung Wilson Wen Bin Goh Franca Ronchese Christiane Ruedl |
| author_sort | Jianpeng Sheng |
| collection | DOAJ |
| description | Dendritic cells residing in the skin represent a large family of antigen-presenting cells, ranging from long-lived Langerhans cells (LC) in the epidermis to various distinct classical dendritic cell subsets in the dermis. Through genetic fate mapping analysis and single-cell RNA-sequencing, we have identified a novel separate population of LC-independent CD207+CD326+ LClike cells in the dermis that homed at a slow rate to the lymph nodes (LNs). These LClike cells are long-lived and radio-resistant but, unlike LCs, they are gradually replenished by bone marrow-derived precursors under steady state. LClike cells together with cDC1s are the main migratory CD207+CD326+ cell fractions present in the LN and not, as currently assumed, LCs, which are barely detectable, if at all. Cutaneous tolerance to haptens depends on LClike cells, whereas LCs suppress effector CD8+ T-cell functions and inflammation locally in the skin during contact hypersensitivity. These findings bring new insights into the dynamism of cutaneous dendritic cells and their function opening novel avenues in the development of treatments to cure inflammatory skin disorders. |
| first_indexed | 2024-12-10T03:50:49Z |
| format | Article |
| id | doaj.art-f57834a1b95f46e2ae2a0aa348b1b2db |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T03:50:49Z |
| publishDate | 2021-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-f57834a1b95f46e2ae2a0aa348b1b2db2022-12-22T02:03:17ZengeLife Sciences Publications LtdeLife2050-084X2021-03-011010.7554/eLife.65412Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell populationJianpeng Sheng0Qi Chen1https://orcid.org/0000-0002-0658-7629Xiaoting Wu2https://orcid.org/0000-0002-0281-8717Yu Wen Dong3Johannes Mayer4https://orcid.org/0000-0001-6225-7803Junlei Zhang5Lin Wang6Xueli Bai7Tingbo Liang8Yang Ho Sung9Wilson Wen Bin Goh10Franca Ronchese11Christiane Ruedl12https://orcid.org/0000-0002-5599-6541Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China; Nanyang Technological University, School of Biological Sciences, Singapore, SingaporeNanyang Technological University, School of Biological Sciences, Singapore, SingaporeNanyang Technological University, School of Biological Sciences, Singapore, SingaporeNanyang Technological University, School of Biological Sciences, Singapore, SingaporeMalaghan Institute of Medical Research, Wellington, New ZealandZhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaNanyang Technological University, School of Biological Sciences, Singapore, SingaporeNanyang Technological University, School of Biological Sciences, Singapore, SingaporeMalaghan Institute of Medical Research, Wellington, New ZealandNanyang Technological University, School of Biological Sciences, Singapore, SingaporeDendritic cells residing in the skin represent a large family of antigen-presenting cells, ranging from long-lived Langerhans cells (LC) in the epidermis to various distinct classical dendritic cell subsets in the dermis. Through genetic fate mapping analysis and single-cell RNA-sequencing, we have identified a novel separate population of LC-independent CD207+CD326+ LClike cells in the dermis that homed at a slow rate to the lymph nodes (LNs). These LClike cells are long-lived and radio-resistant but, unlike LCs, they are gradually replenished by bone marrow-derived precursors under steady state. LClike cells together with cDC1s are the main migratory CD207+CD326+ cell fractions present in the LN and not, as currently assumed, LCs, which are barely detectable, if at all. Cutaneous tolerance to haptens depends on LClike cells, whereas LCs suppress effector CD8+ T-cell functions and inflammation locally in the skin during contact hypersensitivity. These findings bring new insights into the dynamism of cutaneous dendritic cells and their function opening novel avenues in the development of treatments to cure inflammatory skin disorders.https://elifesciences.org/articles/65412skincell migrationdermislymph nodedendritic cellLangerhans cell |
| spellingShingle | Jianpeng Sheng Qi Chen Xiaoting Wu Yu Wen Dong Johannes Mayer Junlei Zhang Lin Wang Xueli Bai Tingbo Liang Yang Ho Sung Wilson Wen Bin Goh Franca Ronchese Christiane Ruedl Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population eLife skin cell migration dermis lymph node dendritic cell Langerhans cell |
| title | Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population |
| title_full | Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population |
| title_fullStr | Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population |
| title_full_unstemmed | Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population |
| title_short | Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population |
| title_sort | fate mapping analysis reveals a novel murine dermal migratory langerhans like cell population |
| topic | skin cell migration dermis lymph node dendritic cell Langerhans cell |
| url | https://elifesciences.org/articles/65412 |
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