In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells
Trichinella spiralis (T. spiralis) adult-specific deoxyribonuclease II-7 (TsDNase II-7), a member of the DNase II-like nuclease family with no DNase II activity, was identified in the excretory–secretory (ES) products of adult worms (AWs). However, its biological functions are still unclear. Our pre...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-06-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10249883/?tool=EBI |
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| author | Jing Wang Xuemin Jin Chengyao Li Xinhui Chen Yanfeng Li Mingyuan Liu Xiaolei Liu Jing Ding |
| author_facet | Jing Wang Xuemin Jin Chengyao Li Xinhui Chen Yanfeng Li Mingyuan Liu Xiaolei Liu Jing Ding |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Trichinella spiralis (T. spiralis) adult-specific deoxyribonuclease II-7 (TsDNase II-7), a member of the DNase II-like nuclease family with no DNase II activity, was identified in the excretory–secretory (ES) products of adult worms (AWs). However, its biological functions are still unclear. Our previous study revealed that TsDNase II-7 is located around the infection site in the intestinal tissue, speculating that it was involved in the T. spiralis invasion of host intestinal epithelial cells (IECs). This study aimed to use RNA interference to verify our speculation that TsDNase II-7 in 3-day old adult T. spiralis (Ad3) plays a role in intestinal invasion. TsDNase II-7-specific small interfering RNAs (siRNAs) were delivered into muscle larvae (MLs) to knockdown TsDNase II-7 expression by electroporation. Twenty-four hours later, the MLs transfected with 2 μM siRNA-841 exhibited decreased in TsDNase II-7 transcription and expression as compared to the control MLs. The knockdown of TsDNase II-7 expression did not affect ML viability, and the low expression of TsDNase II-7 still maintained in Ad3 recovered from TsDNase II-7-RNAi-ML infected mice, resulting in a weakened ability of Ad3 to invade intestinal epithelial cells (IECs). These results indicated that knockdown of TsDNase II-7 gene expression via RNA interference (RNAi) suppressed adult worm invasion and confirmed that TsDNase II-7 plays a crucial role during the intestinal phase of T. spiralis infections, which provided new candidate for vaccine development of T. spiralis. Author summary T. spiralis adult-specific DNase II-7, a member of the DNase II-like nuclease family with no DNase II activity, whose biological functions are still unknown. We previously found that TsDNase II-7 is located around the infection site in the intestinal tissue, speculating that it was involved in the T. spiralis invasion of host intestinal epithelial cells. In this study, the results showed that TsDNase II-7 is highly transcribed and expressed in the adult stage of T. spiralis, and promoted T. spiralis penetration into intestinal epithelial cells, while knockdown of TsDNase II-7 gene expression by siRNA-TsDNase II-7 impaired worm invasive ability. Our study demonstrated the essential role of TsDNase II-7 in invasion and development, highlighting its potential as a molecular target for vaccine development against T. spiralis infection. |
| first_indexed | 2024-03-13T06:16:08Z |
| format | Article |
| id | doaj.art-f57c8b283e2c416ea7850445e4294c1f |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-03-13T06:16:08Z |
| publishDate | 2023-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-f57c8b283e2c416ea7850445e4294c1f2023-06-11T05:31:42ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352023-06-01176In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cellsJing WangXuemin JinChengyao LiXinhui ChenYanfeng LiMingyuan LiuXiaolei LiuJing DingTrichinella spiralis (T. spiralis) adult-specific deoxyribonuclease II-7 (TsDNase II-7), a member of the DNase II-like nuclease family with no DNase II activity, was identified in the excretory–secretory (ES) products of adult worms (AWs). However, its biological functions are still unclear. Our previous study revealed that TsDNase II-7 is located around the infection site in the intestinal tissue, speculating that it was involved in the T. spiralis invasion of host intestinal epithelial cells (IECs). This study aimed to use RNA interference to verify our speculation that TsDNase II-7 in 3-day old adult T. spiralis (Ad3) plays a role in intestinal invasion. TsDNase II-7-specific small interfering RNAs (siRNAs) were delivered into muscle larvae (MLs) to knockdown TsDNase II-7 expression by electroporation. Twenty-four hours later, the MLs transfected with 2 μM siRNA-841 exhibited decreased in TsDNase II-7 transcription and expression as compared to the control MLs. The knockdown of TsDNase II-7 expression did not affect ML viability, and the low expression of TsDNase II-7 still maintained in Ad3 recovered from TsDNase II-7-RNAi-ML infected mice, resulting in a weakened ability of Ad3 to invade intestinal epithelial cells (IECs). These results indicated that knockdown of TsDNase II-7 gene expression via RNA interference (RNAi) suppressed adult worm invasion and confirmed that TsDNase II-7 plays a crucial role during the intestinal phase of T. spiralis infections, which provided new candidate for vaccine development of T. spiralis. Author summary T. spiralis adult-specific DNase II-7, a member of the DNase II-like nuclease family with no DNase II activity, whose biological functions are still unknown. We previously found that TsDNase II-7 is located around the infection site in the intestinal tissue, speculating that it was involved in the T. spiralis invasion of host intestinal epithelial cells. In this study, the results showed that TsDNase II-7 is highly transcribed and expressed in the adult stage of T. spiralis, and promoted T. spiralis penetration into intestinal epithelial cells, while knockdown of TsDNase II-7 gene expression by siRNA-TsDNase II-7 impaired worm invasive ability. Our study demonstrated the essential role of TsDNase II-7 in invasion and development, highlighting its potential as a molecular target for vaccine development against T. spiralis infection.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10249883/?tool=EBI |
| spellingShingle | Jing Wang Xuemin Jin Chengyao Li Xinhui Chen Yanfeng Li Mingyuan Liu Xiaolei Liu Jing Ding In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells PLoS Neglected Tropical Diseases |
| title | In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells |
| title_full | In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells |
| title_fullStr | In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells |
| title_full_unstemmed | In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells |
| title_short | In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells |
| title_sort | in vitro knockdown of tsdnase ii 7 suppresses trichinella spiralis invasion into the host s intestinal epithelial cells |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10249883/?tool=EBI |
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