Effect of palmitoleic acid on the differentiation of bovine skeletal muscle satellite cells
We hypothesized that the unsaturated fatty acid palmitoleic acid (POA) could promote the expression of adipogenic/lipogenic genes in bovine skeletal muscle satellite cells (BSCs). The BSCs were cultured in a growth medium containing 10% fet...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2021-07-01
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| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?pid=jast-63-4-919 |
| _version_ | 1819139539293175808 |
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| author | Junfang Zhang Qiang Li Kim Margarette Corpuz Nogoy Jianfu Sun Bin Sun Ying Wang Lin Tang Jia Yu Xin Jin Xiangzi Li Seong-Ho Choi |
| author_facet | Junfang Zhang Qiang Li Kim Margarette Corpuz Nogoy Jianfu Sun Bin Sun Ying Wang Lin Tang Jia Yu Xin Jin Xiangzi Li Seong-Ho Choi |
| author_sort | Junfang Zhang |
| collection | DOAJ |
| description | We hypothesized that the unsaturated fatty acid palmitoleic acid (POA) could
promote the expression of adipogenic/lipogenic genes in bovine skeletal muscle
satellite cells (BSCs). The BSCs were cultured in a growth medium containing 10%
fetal bovine serum. When the cells reached 80%–90% confluence, we used
the differentiation medium with 5% horse serum for differentiation for 96 h. The
differentiation medium contained 50 µM, 100 µM and 200 µM
POA. Control BSC were cultured only in differentiation media. Compared with the
control BSC, the POA BSC significantly up-regulated the expression of paired box
3 (Pax3) and paired box 7 (Pax7) and
down-regulated myogenin gene expression (p < 0.01),
which indicates a depression in muscle fiber development. However, all POA
treatments up-regulated the expression of the adipocyte transcription factors
peroxisome proliferator-activated receptor γ
(PPARγ), CCAAT/enhancer-binding protein alpha and
beta (C/EBP α and C/EBP β), and
other genes (p < 0.01) and increased the expression of
PAT-family proteins and the concentration of adiponectin in the media. These
results indicate that POA can convert part of BSCs into adipocytes. |
| first_indexed | 2024-12-22T11:24:16Z |
| format | Article |
| id | doaj.art-f57daa29ae924c15a30a600eb1d58dae |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-12-22T11:24:16Z |
| publishDate | 2021-07-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-f57daa29ae924c15a30a600eb1d58dae2022-12-21T18:27:47ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912021-07-0163491993310.5187/jast.2021.e78jast-63-4-919Effect of palmitoleic acid on the differentiation of bovine skeletal muscle satellite cellsJunfang Zhang0Qiang Li1Kim Margarette Corpuz Nogoy2Jianfu Sun3Bin Sun4Ying Wang5Lin Tang6Jia Yu7Xin Jin8Xiangzi Li9Seong-Ho Choi10Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Department of Animal Science, Chungbuk National University, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Department of Animal Science, Chungbuk National University, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Department of Animal Science, Chungbuk National University, We hypothesized that the unsaturated fatty acid palmitoleic acid (POA) could promote the expression of adipogenic/lipogenic genes in bovine skeletal muscle satellite cells (BSCs). The BSCs were cultured in a growth medium containing 10% fetal bovine serum. When the cells reached 80%–90% confluence, we used the differentiation medium with 5% horse serum for differentiation for 96 h. The differentiation medium contained 50 µM, 100 µM and 200 µM POA. Control BSC were cultured only in differentiation media. Compared with the control BSC, the POA BSC significantly up-regulated the expression of paired box 3 (Pax3) and paired box 7 (Pax7) and down-regulated myogenin gene expression (p < 0.01), which indicates a depression in muscle fiber development. However, all POA treatments up-regulated the expression of the adipocyte transcription factors peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein alpha and beta (C/EBP α and C/EBP β), and other genes (p < 0.01) and increased the expression of PAT-family proteins and the concentration of adiponectin in the media. These results indicate that POA can convert part of BSCs into adipocytes.http://www.ejast.org/archive/view_article?pid=jast-63-4-919adipocytespalmitoleic acidsatellite cellsbovinemusclemarbling |
| spellingShingle | Junfang Zhang Qiang Li Kim Margarette Corpuz Nogoy Jianfu Sun Bin Sun Ying Wang Lin Tang Jia Yu Xin Jin Xiangzi Li Seong-Ho Choi Effect of palmitoleic acid on the differentiation of bovine skeletal muscle satellite cells Journal of Animal Science and Technology adipocytes palmitoleic acid satellite cells bovine muscle marbling |
| title | Effect of palmitoleic acid on the differentiation of bovine skeletal
muscle satellite cells |
| title_full | Effect of palmitoleic acid on the differentiation of bovine skeletal
muscle satellite cells |
| title_fullStr | Effect of palmitoleic acid on the differentiation of bovine skeletal
muscle satellite cells |
| title_full_unstemmed | Effect of palmitoleic acid on the differentiation of bovine skeletal
muscle satellite cells |
| title_short | Effect of palmitoleic acid on the differentiation of bovine skeletal
muscle satellite cells |
| title_sort | effect of palmitoleic acid on the differentiation of bovine skeletal muscle satellite cells |
| topic | adipocytes palmitoleic acid satellite cells bovine muscle marbling |
| url | http://www.ejast.org/archive/view_article?pid=jast-63-4-919 |
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