Agrobacterium tumefaciens estC, Encoding an Enzyme Containing Esterase Activity, Is Regulated by EstR, a Regulator in the MarR Family.
Analysis of the A. tumefaciens genome revealed estC, which encodes an esterase located next to its transcriptional regulator estR, a regulator of esterase in the MarR family. Inactivation of estC results in a small increase in the resistance to organic hydroperoxides, whereas a high level of express...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5201249?pdf=render |
| _version_ | 1818308762765623296 |
|---|---|
| author | Surawach Rittiroongrad Nisanart Charoenlap Suparat Giengkam Paiboon Vattanaviboon Skorn Mongkolsuk |
| author_facet | Surawach Rittiroongrad Nisanart Charoenlap Suparat Giengkam Paiboon Vattanaviboon Skorn Mongkolsuk |
| author_sort | Surawach Rittiroongrad |
| collection | DOAJ |
| description | Analysis of the A. tumefaciens genome revealed estC, which encodes an esterase located next to its transcriptional regulator estR, a regulator of esterase in the MarR family. Inactivation of estC results in a small increase in the resistance to organic hydroperoxides, whereas a high level of expression of estC from an expression vector leads to a reduction in the resistance to organic hydroperoxides and menadione. The estC gene is transcribed divergently from its regulator, estR. Expression analysis showed that only high concentrations of cumene hydroperoxide (CHP, 1 mM) induced expression of both genes in an EstR-dependent manner. The EstR protein acts as a CHP sensor and a transcriptional repressor of both genes. EstR specifically binds to the operator sites OI and OII overlapping the promoter elements of estC and estR. This binding is responsible for transcription repression of both genes. Exposure to organic hydroperoxide results in oxidation of the sensing cysteine (Cys16) residue of EstR, leading to a release of the oxidized repressor from the operator sites, thereby allowing transcription and high levels of expression of both genes. The estC is the first organic hydroperoxide-inducible esterase-encoding gene in alphaproteobacteria. |
| first_indexed | 2024-12-13T07:19:26Z |
| format | Article |
| id | doaj.art-f580edbec3b640ea8967a0e051589046 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T07:19:26Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f580edbec3b640ea8967a0e0515890462022-12-21T23:55:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016879110.1371/journal.pone.0168791Agrobacterium tumefaciens estC, Encoding an Enzyme Containing Esterase Activity, Is Regulated by EstR, a Regulator in the MarR Family.Surawach RittiroongradNisanart CharoenlapSuparat GiengkamPaiboon VattanaviboonSkorn MongkolsukAnalysis of the A. tumefaciens genome revealed estC, which encodes an esterase located next to its transcriptional regulator estR, a regulator of esterase in the MarR family. Inactivation of estC results in a small increase in the resistance to organic hydroperoxides, whereas a high level of expression of estC from an expression vector leads to a reduction in the resistance to organic hydroperoxides and menadione. The estC gene is transcribed divergently from its regulator, estR. Expression analysis showed that only high concentrations of cumene hydroperoxide (CHP, 1 mM) induced expression of both genes in an EstR-dependent manner. The EstR protein acts as a CHP sensor and a transcriptional repressor of both genes. EstR specifically binds to the operator sites OI and OII overlapping the promoter elements of estC and estR. This binding is responsible for transcription repression of both genes. Exposure to organic hydroperoxide results in oxidation of the sensing cysteine (Cys16) residue of EstR, leading to a release of the oxidized repressor from the operator sites, thereby allowing transcription and high levels of expression of both genes. The estC is the first organic hydroperoxide-inducible esterase-encoding gene in alphaproteobacteria.http://europepmc.org/articles/PMC5201249?pdf=render |
| spellingShingle | Surawach Rittiroongrad Nisanart Charoenlap Suparat Giengkam Paiboon Vattanaviboon Skorn Mongkolsuk Agrobacterium tumefaciens estC, Encoding an Enzyme Containing Esterase Activity, Is Regulated by EstR, a Regulator in the MarR Family. PLoS ONE |
| title | Agrobacterium tumefaciens estC, Encoding an Enzyme Containing Esterase Activity, Is Regulated by EstR, a Regulator in the MarR Family. |
| title_full | Agrobacterium tumefaciens estC, Encoding an Enzyme Containing Esterase Activity, Is Regulated by EstR, a Regulator in the MarR Family. |
| title_fullStr | Agrobacterium tumefaciens estC, Encoding an Enzyme Containing Esterase Activity, Is Regulated by EstR, a Regulator in the MarR Family. |
| title_full_unstemmed | Agrobacterium tumefaciens estC, Encoding an Enzyme Containing Esterase Activity, Is Regulated by EstR, a Regulator in the MarR Family. |
| title_short | Agrobacterium tumefaciens estC, Encoding an Enzyme Containing Esterase Activity, Is Regulated by EstR, a Regulator in the MarR Family. |
| title_sort | agrobacterium tumefaciens estc encoding an enzyme containing esterase activity is regulated by estr a regulator in the marr family |
| url | http://europepmc.org/articles/PMC5201249?pdf=render |
| work_keys_str_mv | AT surawachrittiroongrad agrobacteriumtumefaciensestcencodinganenzymecontainingesteraseactivityisregulatedbyestraregulatorinthemarrfamily AT nisanartcharoenlap agrobacteriumtumefaciensestcencodinganenzymecontainingesteraseactivityisregulatedbyestraregulatorinthemarrfamily AT suparatgiengkam agrobacteriumtumefaciensestcencodinganenzymecontainingesteraseactivityisregulatedbyestraregulatorinthemarrfamily AT paiboonvattanaviboon agrobacteriumtumefaciensestcencodinganenzymecontainingesteraseactivityisregulatedbyestraregulatorinthemarrfamily AT skornmongkolsuk agrobacteriumtumefaciensestcencodinganenzymecontainingesteraseactivityisregulatedbyestraregulatorinthemarrfamily |