A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2–10 ng/mL at initial biopsy
Abstract The aim of this study was to identify a urine extracellular vesicle circular RNA (circRNA) classifier that could detect high-grade prostate cancer (PCa) of Grade Group (GG) 2 or greater. For this purpose, we used RNA sequencing to identify candidate circRNAs from urinary extracellular vesic...
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12943-021-01388-6 |
| _version_ | 1818453812835254272 |
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| author | Ya-Di He Wen Tao Tao He Bang-Yu Wang Xiu-Mei Tang Liang-Ming Zhang Zhen-Quan Wu Wei-Ming Deng Ling-Xiao Zhang Chun-Kui Shao Jing Zhou Li-Min Rong Xin Gao Liao-Yuan Li |
| author_facet | Ya-Di He Wen Tao Tao He Bang-Yu Wang Xiu-Mei Tang Liang-Ming Zhang Zhen-Quan Wu Wei-Ming Deng Ling-Xiao Zhang Chun-Kui Shao Jing Zhou Li-Min Rong Xin Gao Liao-Yuan Li |
| author_sort | Ya-Di He |
| collection | DOAJ |
| description | Abstract The aim of this study was to identify a urine extracellular vesicle circular RNA (circRNA) classifier that could detect high-grade prostate cancer (PCa) of Grade Group (GG) 2 or greater. For this purpose, we used RNA sequencing to identify candidate circRNAs from urinary extracellular vesicles from 11 patients with high-grade PCa and 11 case-matched patients with benign prostatic hyperplasia. Using ddPCR in a training cohort (n = 263), we built a urine extracellular vesicle circRNA classifier (Ccirc, containing circPDLIM5, circSCAF8, circPLXDC2, circSCAMP1, and circCCNT2), which was evaluated in two independent cohorts (n = 497, n = 505). Ccirc showed higher accuracy than two standard of care risk calculators (RCs) (PCPT-RC 2.0 and ERSPC-RC) in both the training cohort and the validation cohorts. In all three cohorts, this novel urine extracellular vesicle circRNA classifier plus RCs was statistically more predictive than RCs alone for predicting ≥ GG2 PCa. This assay, which does not require precollection digital rectal examination nor special handling, is repeatable, noninvasive, and can be easily implemented as part of the basic clinical workflow. |
| first_indexed | 2024-12-14T21:44:56Z |
| format | Article |
| id | doaj.art-f58307fb212a4e9a89bcff11dd042b0a |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-14T21:44:56Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-f58307fb212a4e9a89bcff11dd042b0a2022-12-21T22:46:22ZengBMCMolecular Cancer1476-45982021-07-012011610.1186/s12943-021-01388-6A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2–10 ng/mL at initial biopsyYa-Di He0Wen Tao1Tao He2Bang-Yu Wang3Xiu-Mei Tang4Liang-Ming Zhang5Zhen-Quan Wu6Wei-Ming Deng7Ling-Xiao Zhang8Chun-Kui Shao9Jing Zhou10Li-Min Rong11Xin Gao12Liao-Yuan Li13Centre of Physical Examination, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Urology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Urology, The Third Affiliated Hospital, Sun Yat-Sen UniversityBreast Surgery, The Third Affiliated Hospital, Sun Yat-Sen UniversityCentre of Physical Examination, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Spine Surgery, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Urology, Foshan First Municipal People’s Hospital, Sun Yat-Sen UniversityDepartment of Urology, The First Affiliated Hospital, University of South ChinaDepartment of Urology, The First Affiliated Hospital, Hainan Medical CollegeDepartment of Pathology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Pathology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Spine Surgery, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Urology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Urology, The Third Affiliated Hospital, Sun Yat-Sen UniversityAbstract The aim of this study was to identify a urine extracellular vesicle circular RNA (circRNA) classifier that could detect high-grade prostate cancer (PCa) of Grade Group (GG) 2 or greater. For this purpose, we used RNA sequencing to identify candidate circRNAs from urinary extracellular vesicles from 11 patients with high-grade PCa and 11 case-matched patients with benign prostatic hyperplasia. Using ddPCR in a training cohort (n = 263), we built a urine extracellular vesicle circRNA classifier (Ccirc, containing circPDLIM5, circSCAF8, circPLXDC2, circSCAMP1, and circCCNT2), which was evaluated in two independent cohorts (n = 497, n = 505). Ccirc showed higher accuracy than two standard of care risk calculators (RCs) (PCPT-RC 2.0 and ERSPC-RC) in both the training cohort and the validation cohorts. In all three cohorts, this novel urine extracellular vesicle circRNA classifier plus RCs was statistically more predictive than RCs alone for predicting ≥ GG2 PCa. This assay, which does not require precollection digital rectal examination nor special handling, is repeatable, noninvasive, and can be easily implemented as part of the basic clinical workflow.https://doi.org/10.1186/s12943-021-01388-6Prostate cancerCircular RNA (circRNA)DiagnosisUrineExtracellular vesicle |
| spellingShingle | Ya-Di He Wen Tao Tao He Bang-Yu Wang Xiu-Mei Tang Liang-Ming Zhang Zhen-Quan Wu Wei-Ming Deng Ling-Xiao Zhang Chun-Kui Shao Jing Zhou Li-Min Rong Xin Gao Liao-Yuan Li A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2–10 ng/mL at initial biopsy Molecular Cancer Prostate cancer Circular RNA (circRNA) Diagnosis Urine Extracellular vesicle |
| title | A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2–10 ng/mL at initial biopsy |
| title_full | A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2–10 ng/mL at initial biopsy |
| title_fullStr | A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2–10 ng/mL at initial biopsy |
| title_full_unstemmed | A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2–10 ng/mL at initial biopsy |
| title_short | A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2–10 ng/mL at initial biopsy |
| title_sort | urine extracellular vesicle circrna classifier for detection of high grade prostate cancer in patients with prostate specific antigen 2 10 ng ml at initial biopsy |
| topic | Prostate cancer Circular RNA (circRNA) Diagnosis Urine Extracellular vesicle |
| url | https://doi.org/10.1186/s12943-021-01388-6 |
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