Mechanical effects on KATP channel gating in rat ventricular myocytes.
Cardiac KATP channels link metabolism with electrical activity. They are implicated in arrhythmias, secretion of atrial natriuretic peptide and protection of the heart from hypertrophy and failure. These processes may involve mechanosensitivity. KATP channels can be activated by mechanical stimulati...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3653899?pdf=render |
| _version_ | 1818470730827825152 |
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| author | Haixia Huang Lifang Liang Ping Liu Hua Wei Frederick Sachs Weizhen Niu Wei Wang |
| author_facet | Haixia Huang Lifang Liang Ping Liu Hua Wei Frederick Sachs Weizhen Niu Wei Wang |
| author_sort | Haixia Huang |
| collection | DOAJ |
| description | Cardiac KATP channels link metabolism with electrical activity. They are implicated in arrhythmias, secretion of atrial natriuretic peptide and protection of the heart from hypertrophy and failure. These processes may involve mechanosensitivity. KATP channels can be activated by mechanical stimulation and disrupting the cortical actin increases the activity. We propose that KATP channels are modulated by local bilayer tension and this tension is affected by cortical F-actin. Here we measured KATP background activity and stretch sensitivity with inside-out patches of rat ventricular myocytes before and after disrupting F-actin. Disrupting F-actin potentiated background activity but did not influence the slope sensitivity in the semilog relationship of NPo vs. suction that is a measure of the change in dimensions between closed and open states. Thus actin alters prestress on the channel probably by parallel elastic sharing of mean cortical tension with the bilayer. |
| first_indexed | 2024-04-13T21:42:09Z |
| format | Article |
| id | doaj.art-f5833cedfd874289bfae299d345dc695 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T21:42:09Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f5833cedfd874289bfae299d345dc6952022-12-22T02:28:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6333710.1371/journal.pone.0063337Mechanical effects on KATP channel gating in rat ventricular myocytes.Haixia HuangLifang LiangPing LiuHua WeiFrederick SachsWeizhen NiuWei WangCardiac KATP channels link metabolism with electrical activity. They are implicated in arrhythmias, secretion of atrial natriuretic peptide and protection of the heart from hypertrophy and failure. These processes may involve mechanosensitivity. KATP channels can be activated by mechanical stimulation and disrupting the cortical actin increases the activity. We propose that KATP channels are modulated by local bilayer tension and this tension is affected by cortical F-actin. Here we measured KATP background activity and stretch sensitivity with inside-out patches of rat ventricular myocytes before and after disrupting F-actin. Disrupting F-actin potentiated background activity but did not influence the slope sensitivity in the semilog relationship of NPo vs. suction that is a measure of the change in dimensions between closed and open states. Thus actin alters prestress on the channel probably by parallel elastic sharing of mean cortical tension with the bilayer.http://europepmc.org/articles/PMC3653899?pdf=render |
| spellingShingle | Haixia Huang Lifang Liang Ping Liu Hua Wei Frederick Sachs Weizhen Niu Wei Wang Mechanical effects on KATP channel gating in rat ventricular myocytes. PLoS ONE |
| title | Mechanical effects on KATP channel gating in rat ventricular myocytes. |
| title_full | Mechanical effects on KATP channel gating in rat ventricular myocytes. |
| title_fullStr | Mechanical effects on KATP channel gating in rat ventricular myocytes. |
| title_full_unstemmed | Mechanical effects on KATP channel gating in rat ventricular myocytes. |
| title_short | Mechanical effects on KATP channel gating in rat ventricular myocytes. |
| title_sort | mechanical effects on katp channel gating in rat ventricular myocytes |
| url | http://europepmc.org/articles/PMC3653899?pdf=render |
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