Uncleavable Nup98–Nup96 is functional in the fission yeastSchizosaccharomyces pombe
Essential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98–Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98–N...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.fob.2015.06.004 |
| _version_ | 1811206026010034176 |
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| author | Haruhiko Asakawa Chie Mori Chizuru Ohtsuki Masaaki Iwamoto Yasushi Hiraoka Tokuko Haraguchi |
| author_facet | Haruhiko Asakawa Chie Mori Chizuru Ohtsuki Masaaki Iwamoto Yasushi Hiraoka Tokuko Haraguchi |
| author_sort | Haruhiko Asakawa |
| collection | DOAJ |
| description | Essential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98–Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98–Nup96 joint molecule as a sole protein product of thenup189+ gene in the fission yeastSchizosaccharomyces pombe. Cells expressing only the joint molecule were found to be viable. This result indicates that autocleavage of Nup98–Nup96 is dispensable for cell growth, at least under normal culture conditions inS. pombe. |
| first_indexed | 2024-04-12T03:41:35Z |
| format | Article |
| id | doaj.art-f5884c3021f8485f8e1b09c0cbeb5ad8 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-12T03:41:35Z |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-f5884c3021f8485f8e1b09c0cbeb5ad82022-12-22T03:49:17ZengWileyFEBS Open Bio2211-54632015-01-015150851410.1016/j.fob.2015.06.004Uncleavable Nup98–Nup96 is functional in the fission yeastSchizosaccharomyces pombeHaruhiko Asakawa0Chie Mori1Chizuru Ohtsuki2Masaaki Iwamoto3Yasushi Hiraoka4Tokuko Haraguchi5Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita 565-0871, JapanAdvanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, JapanGraduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita 565-0871, JapanAdvanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, JapanGraduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita 565-0871, JapanGraduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita 565-0871, JapanEssential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98–Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98–Nup96 joint molecule as a sole protein product of thenup189+ gene in the fission yeastSchizosaccharomyces pombe. Cells expressing only the joint molecule were found to be viable. This result indicates that autocleavage of Nup98–Nup96 is dispensable for cell growth, at least under normal culture conditions inS. pombe.https://doi.org/10.1016/j.fob.2015.06.004NucleoporinsNuclear pore complexFG nupNup107-160 complexAlternative splicingFission yeast |
| spellingShingle | Haruhiko Asakawa Chie Mori Chizuru Ohtsuki Masaaki Iwamoto Yasushi Hiraoka Tokuko Haraguchi Uncleavable Nup98–Nup96 is functional in the fission yeastSchizosaccharomyces pombe FEBS Open Bio Nucleoporins Nuclear pore complex FG nup Nup107-160 complex Alternative splicing Fission yeast |
| title | Uncleavable Nup98–Nup96 is functional in the fission yeastSchizosaccharomyces pombe |
| title_full | Uncleavable Nup98–Nup96 is functional in the fission yeastSchizosaccharomyces pombe |
| title_fullStr | Uncleavable Nup98–Nup96 is functional in the fission yeastSchizosaccharomyces pombe |
| title_full_unstemmed | Uncleavable Nup98–Nup96 is functional in the fission yeastSchizosaccharomyces pombe |
| title_short | Uncleavable Nup98–Nup96 is functional in the fission yeastSchizosaccharomyces pombe |
| title_sort | uncleavable nup98 nup96 is functional in the fission yeastschizosaccharomyces pombe |
| topic | Nucleoporins Nuclear pore complex FG nup Nup107-160 complex Alternative splicing Fission yeast |
| url | https://doi.org/10.1016/j.fob.2015.06.004 |
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