A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to <i>Rosellinia necatrix</i> Exudate
Avocado embryogenic cultures were selected for resistance to the culture filtrate (CF) of <i>Rosellinia necatrix</i>, the causal agent of White Root Rot disease. A resistant callus line was obtained through recurrent selections in progressively increasing concentrations of fungal CF (fro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/5/1354 |
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| author | Ana Moreno-Pérez Adela Zumaquero Elsa Martínez-Ferri Carlos López-Herrera Fernando Pliego-Alfaro Elena Palomo-Ríos Clara Pliego |
| author_facet | Ana Moreno-Pérez Adela Zumaquero Elsa Martínez-Ferri Carlos López-Herrera Fernando Pliego-Alfaro Elena Palomo-Ríos Clara Pliego |
| author_sort | Ana Moreno-Pérez |
| collection | DOAJ |
| description | Avocado embryogenic cultures were selected for resistance to the culture filtrate (CF) of <i>Rosellinia necatrix</i>, the causal agent of White Root Rot disease. A resistant callus line was obtained through recurrent selections in progressively increasing concentrations of fungal CF (from 60% to 80%). RNA sequencing (RNA-Seq) technology was used to compare the transcriptomic profiles of the avocado embryogenic-callus-resistant line L3 (capable to survive in the presence of 80% CF) and control line AN-9 (not exposed to CF), after 24 h of growth in a medium containing 40% CF. A total of 25,211 transcripts were obtained, of which 4,918 and 5,716 were differentially expressed in the resistant and control line, respectively. Interestingly, exposure of embryogenic callus lines to 40% of <i>R. necatrix</i> exudates induced genes previously reported to be related to avocado defense against fungal diseases (lignin biosynthesis, Pathogenesis Related (PR) proteins, WRKY (WRKYGQK) Transcription Factor (TF), NAC (NAM, ATAF1/2, and CUC2) TF, proteinase inhibitors and Ethylene Response Transcription Factor (ERF), among others), which were accumulated in greater amounts in the resistant line in comparison to the susceptible one. This research will contribute to the understanding of avocado defense against this pathogen, thereby aiding in the selection of resistant avocado rootstocks. |
| first_indexed | 2024-03-11T04:02:11Z |
| format | Article |
| id | doaj.art-f589f3cfc159438ebf4ab54789f15e5f |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T04:02:11Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-f589f3cfc159438ebf4ab54789f15e5f2023-11-18T00:07:00ZengMDPI AGAgronomy2073-43952023-05-01135135410.3390/agronomy13051354A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to <i>Rosellinia necatrix</i> ExudateAna Moreno-Pérez0Adela Zumaquero1Elsa Martínez-Ferri2Carlos López-Herrera3Fernando Pliego-Alfaro4Elena Palomo-Ríos5Clara Pliego6Department of Genomics and Biotechnology (IFAPA), Fruticultura Subtropical y Mediterránea Unidad Asociada de I + D + i al CSIC, 29140 Málaga, SpainDepartment of Genomics and Biotechnology (IFAPA), Fruticultura Subtropical y Mediterránea Unidad Asociada de I + D + i al CSIC, 29140 Málaga, SpainDepartment of Crop Ecophysiology (IFAPA), Fruticultura Subtropical y Mediterránea Unidad Asociada de I + D + i al CSIC, 29140 Málaga, SpainInstitute for Sustainable Agriculture, Spanish Research Council, CSIC, 14004 Córdoba, SpainDepartment of Botany and Plant Physiology, IHSM-UMA-CSIC La Mayora, 29010 Málaga, SpainDepartment of Botany and Plant Physiology, IHSM-UMA-CSIC La Mayora, 29010 Málaga, SpainDepartment of Genomics and Biotechnology (IFAPA), Fruticultura Subtropical y Mediterránea Unidad Asociada de I + D + i al CSIC, 29140 Málaga, SpainAvocado embryogenic cultures were selected for resistance to the culture filtrate (CF) of <i>Rosellinia necatrix</i>, the causal agent of White Root Rot disease. A resistant callus line was obtained through recurrent selections in progressively increasing concentrations of fungal CF (from 60% to 80%). RNA sequencing (RNA-Seq) technology was used to compare the transcriptomic profiles of the avocado embryogenic-callus-resistant line L3 (capable to survive in the presence of 80% CF) and control line AN-9 (not exposed to CF), after 24 h of growth in a medium containing 40% CF. A total of 25,211 transcripts were obtained, of which 4,918 and 5,716 were differentially expressed in the resistant and control line, respectively. Interestingly, exposure of embryogenic callus lines to 40% of <i>R. necatrix</i> exudates induced genes previously reported to be related to avocado defense against fungal diseases (lignin biosynthesis, Pathogenesis Related (PR) proteins, WRKY (WRKYGQK) Transcription Factor (TF), NAC (NAM, ATAF1/2, and CUC2) TF, proteinase inhibitors and Ethylene Response Transcription Factor (ERF), among others), which were accumulated in greater amounts in the resistant line in comparison to the susceptible one. This research will contribute to the understanding of avocado defense against this pathogen, thereby aiding in the selection of resistant avocado rootstocks.https://www.mdpi.com/2073-4395/13/5/1354avocadoembryogenic culture<i>Rosellinia necatrix</i>resistant genotypestranscriptomic analysis |
| spellingShingle | Ana Moreno-Pérez Adela Zumaquero Elsa Martínez-Ferri Carlos López-Herrera Fernando Pliego-Alfaro Elena Palomo-Ríos Clara Pliego A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to <i>Rosellinia necatrix</i> Exudate Agronomy avocado embryogenic culture <i>Rosellinia necatrix</i> resistant genotypes transcriptomic analysis |
| title | A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to <i>Rosellinia necatrix</i> Exudate |
| title_full | A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to <i>Rosellinia necatrix</i> Exudate |
| title_fullStr | A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to <i>Rosellinia necatrix</i> Exudate |
| title_full_unstemmed | A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to <i>Rosellinia necatrix</i> Exudate |
| title_short | A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to <i>Rosellinia necatrix</i> Exudate |
| title_sort | comparative transcriptome analysis of avocado embryogenic lines susceptible or resistant to i rosellinia necatrix i exudate |
| topic | avocado embryogenic culture <i>Rosellinia necatrix</i> resistant genotypes transcriptomic analysis |
| url | https://www.mdpi.com/2073-4395/13/5/1354 |
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