Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of Probiotics
Wild-type strains hardly meet the current industrial demands due to low stability. The performance of microbial strains (high-yielding, high-quality strains) can be improved by using physical mutagenesis techniques. At the same time，high throughput screening methods and techniques can be used to qui...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2023-07-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090175 |
| _version_ | 1797737884594208768 |
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| author | Lina PAN Rongxue TANG Wenli KANG Yisi LI Pengyu HU Jiaqi WANG Hongbo ZHOU |
| author_facet | Lina PAN Rongxue TANG Wenli KANG Yisi LI Pengyu HU Jiaqi WANG Hongbo ZHOU |
| author_sort | Lina PAN |
| collection | DOAJ |
| description | Wild-type strains hardly meet the current industrial demands due to low stability. The performance of microbial strains (high-yielding, high-quality strains) can be improved by using physical mutagenesis techniques. At the same time，high throughput screening methods and techniques can be used to quickly obtain ideal strains from the mutation library. However, traditional manual screening and shaker culture are high-cost, time-consuming, and laborious, and high-throughput screening technology can solve this problem. In this review, the applications of traditional and novel mutagenesis used for the improvement of probiotics are summarized, the principles of traditional and novel physical mutagenesis techniques are discussed, the differences between the two kinds of mutagenesis techniques are compared and their applications in the improvement of probiotics are described. At the same time, the characteristics and relevant applications of various high-throughput screening technologies (microtitration plate screening, fluorescence activated cell sorting, biosensors screening, droplet microfluidic platform screening and model animal platform screening) are summarized. This review provides important reference for reducing screening cost, improving screening efficiency and obtaining high yield ideal target strains. |
| first_indexed | 2024-03-12T13:34:55Z |
| format | Article |
| id | doaj.art-f58d363d1df645c6b4e096bf82b2d1d4 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:55Z |
| publishDate | 2023-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-f58d363d1df645c6b4e096bf82b2d1d42023-08-24T06:00:44ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-07-01441345846510.13386/j.issn1002-0306.20220901752022090175-13Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of ProbioticsLina PAN0Rongxue TANG1Wenli KANG2Yisi LI3Pengyu HU4Jiaqi WANG5Hongbo ZHOU6School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, ChinaAusnutria Dairy (China) Co., Ltd., Changsha 410127, ChinaAusnutria Dairy (China) Co., Ltd., Changsha 410127, ChinaAusnutria Dairy (China) Co., Ltd., Changsha 410127, ChinaAusnutria Dairy (China) Co., Ltd., Changsha 410127, ChinaAusnutria Dairy (China) Co., Ltd., Changsha 410127, ChinaSchool of Minerals Processing and Bioengineering, Central South University, Changsha 410083, ChinaWild-type strains hardly meet the current industrial demands due to low stability. The performance of microbial strains (high-yielding, high-quality strains) can be improved by using physical mutagenesis techniques. At the same time，high throughput screening methods and techniques can be used to quickly obtain ideal strains from the mutation library. However, traditional manual screening and shaker culture are high-cost, time-consuming, and laborious, and high-throughput screening technology can solve this problem. In this review, the applications of traditional and novel mutagenesis used for the improvement of probiotics are summarized, the principles of traditional and novel physical mutagenesis techniques are discussed, the differences between the two kinds of mutagenesis techniques are compared and their applications in the improvement of probiotics are described. At the same time, the characteristics and relevant applications of various high-throughput screening technologies (microtitration plate screening, fluorescence activated cell sorting, biosensors screening, droplet microfluidic platform screening and model animal platform screening) are summarized. This review provides important reference for reducing screening cost, improving screening efficiency and obtaining high yield ideal target strains.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090175physical mutagenesishigh-throughput screeningprobioticsapplication |
| spellingShingle | Lina PAN Rongxue TANG Wenli KANG Yisi LI Pengyu HU Jiaqi WANG Hongbo ZHOU Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of Probiotics Shipin gongye ke-ji physical mutagenesis high-throughput screening probiotics application |
| title | Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of Probiotics |
| title_full | Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of Probiotics |
| title_fullStr | Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of Probiotics |
| title_full_unstemmed | Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of Probiotics |
| title_short | Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of Probiotics |
| title_sort | application of physical mutagenesis and high throughput screening technology in the selection of probiotics |
| topic | physical mutagenesis high-throughput screening probiotics application |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090175 |
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