Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-08-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731418794007 |
| _version_ | 1828435638077095936 |
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| author | Shona J Waddell María C de Andrés Penelope M Tsimbouri Enateri V Alakpa Maggie Cusack Matthew J Dalby Richard OC Oreffo |
| author_facet | Shona J Waddell María C de Andrés Penelope M Tsimbouri Enateri V Alakpa Maggie Cusack Matthew J Dalby Richard OC Oreffo |
| author_sort | Shona J Waddell |
| collection | DOAJ |
| description | The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP ( p < 0.05) and OCN ( p < 0.01) and increased metabolite intensity ( p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity ( p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype. |
| first_indexed | 2024-12-10T19:10:47Z |
| format | Article |
| id | doaj.art-f58e2937146846a3a4c323e66e1852de |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-10T19:10:47Z |
| publishDate | 2018-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-f58e2937146846a3a4c323e66e1852de2022-12-22T01:36:45ZengSAGE PublishingJournal of Tissue Engineering2041-73142018-08-01910.1177/2041731418794007Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cellsShona J Waddell0María C de Andrés1Penelope M Tsimbouri2Enateri V Alakpa3Maggie Cusack4Matthew J Dalby5Richard OC Oreffo6Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, Faculty of Medicine, University of Southampton, Southampton, UKCentre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, Faculty of Medicine, University of Southampton, Southampton, UKCentre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, CMVLS, University of Glasgow, Glasgow, UKDepartment of Integrative Medical Biology, Umeå University, Umeå, SwedenDivision of Biological and Environmental Science, University of Stirling, Stirling, UKCentre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, CMVLS, University of Glasgow, Glasgow, UKCentre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, Faculty of Medicine, University of Southampton, Southampton, UKThe regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP ( p < 0.05) and OCN ( p < 0.01) and increased metabolite intensity ( p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity ( p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype.https://doi.org/10.1177/2041731418794007 |
| spellingShingle | Shona J Waddell María C de Andrés Penelope M Tsimbouri Enateri V Alakpa Maggie Cusack Matthew J Dalby Richard OC Oreffo Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells Journal of Tissue Engineering |
| title | Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells |
| title_full | Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells |
| title_fullStr | Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells |
| title_full_unstemmed | Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells |
| title_short | Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells |
| title_sort | biomimetic oyster shell replicated topography alters the behaviour of human skeletal stem cells |
| url | https://doi.org/10.1177/2041731418794007 |
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