CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation
ABSTRACT: Objectives: Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cell possessing self-renewal and multilineage differentiation capabilities. The dysfunction of DPSCs is related to the pathologic process of pulpitis. The participation of circular RNAs (circRNAs) in DPSC differenti...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | International Dental Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0020653922001939 |
| _version_ | 1797825636491853824 |
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| author | Cuiwei Liang Wenmiao Li Qian Huang Qitao Wen |
| author_facet | Cuiwei Liang Wenmiao Li Qian Huang Qitao Wen |
| author_sort | Cuiwei Liang |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cell possessing self-renewal and multilineage differentiation capabilities. The dysfunction of DPSCs is related to the pathologic process of pulpitis. The participation of circular RNAs (circRNAs) in DPSC differentiation has been identified. This work focussed on exploring the functions and mechanism of circFKBP5 in DPSC dysfunction evoked by lipopolysaccharide (LPS). Materials and methods: The viability and apoptosis of human DPSCs (hDPSCs) were determined using Cell Counting Kit-8 assay and flow cytometry. Inflammation was analysed by measuring the release of inflammatory cytokines. The osteogenic differentiation of hDPSCs was investigated by performing alkaline phosphatase (ALP) staining and alizarin red S staining and detecting the changes of ALP and runt-related transcription factor 2 (RUNX2) proteins. The dual-luciferase reporter, RNA immunoprecipitation (RIP), and pull-down assays were used to confirm the binding between miR-708-5p and circFKBP5 or G-protein-coupled receptor (GPCR)–kinase interacting protein 2 (GIT2). Results: CircFKBP5 expression was decreased in hDPSCs and, functionally, reexpression of circFKBP5 attenuated LPS-induced apoptosis, inflammation, and inhibition of proliferation ability and osteogenic differentiation in hDPSCs. Mechanistically, circFKBP5 acted as a sponge for miR-708-5p, which was verified to target GIT2. LPS induced miR-708-5p expression in hDPSCs, and knockdown of miR-708-5p protected against LPS-evoked hDPSC dysfunction. Besides, GIT2 expression was decreased in hDPSCs after LPS treatment. Rescue experiments showed that GIT2 could mediate the protective functions of circFKBP5 on hDPSCs under LPS treatment. Conclusions: CircFKBP5 could protect against LPS-induced apoptosis, inflammation, and osteogenic differentiation inhibition in hDPSCs via the miR-708-5p/GIT2 axis. |
| first_indexed | 2024-03-13T10:56:52Z |
| format | Article |
| id | doaj.art-f591712d833c45adae771583aee85437 |
| institution | Directory Open Access Journal |
| issn | 0020-6539 |
| language | English |
| last_indexed | 2024-03-13T10:56:52Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Dental Journal |
| spelling | doaj.art-f591712d833c45adae771583aee854372023-05-17T04:22:20ZengElsevierInternational Dental Journal0020-65392023-06-01733377386CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic DifferentiationCuiwei Liang0Wenmiao Li1Qian Huang2Qitao Wen3Department of Prosthodontics, Hainan Stomatological Hospital, Haikou, Hainan, ChinaDental Department, Central South University Xiang Ya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan, ChinaDental Department, Central South University Xiang Ya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan, ChinaDental Department, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, Hainan, China; Corresponding author: Dental Department, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), N0. 19 Xiuhua Road, Xiuying District, Haikou, Hainan, 570100, China.ABSTRACT: Objectives: Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cell possessing self-renewal and multilineage differentiation capabilities. The dysfunction of DPSCs is related to the pathologic process of pulpitis. The participation of circular RNAs (circRNAs) in DPSC differentiation has been identified. This work focussed on exploring the functions and mechanism of circFKBP5 in DPSC dysfunction evoked by lipopolysaccharide (LPS). Materials and methods: The viability and apoptosis of human DPSCs (hDPSCs) were determined using Cell Counting Kit-8 assay and flow cytometry. Inflammation was analysed by measuring the release of inflammatory cytokines. The osteogenic differentiation of hDPSCs was investigated by performing alkaline phosphatase (ALP) staining and alizarin red S staining and detecting the changes of ALP and runt-related transcription factor 2 (RUNX2) proteins. The dual-luciferase reporter, RNA immunoprecipitation (RIP), and pull-down assays were used to confirm the binding between miR-708-5p and circFKBP5 or G-protein-coupled receptor (GPCR)–kinase interacting protein 2 (GIT2). Results: CircFKBP5 expression was decreased in hDPSCs and, functionally, reexpression of circFKBP5 attenuated LPS-induced apoptosis, inflammation, and inhibition of proliferation ability and osteogenic differentiation in hDPSCs. Mechanistically, circFKBP5 acted as a sponge for miR-708-5p, which was verified to target GIT2. LPS induced miR-708-5p expression in hDPSCs, and knockdown of miR-708-5p protected against LPS-evoked hDPSC dysfunction. Besides, GIT2 expression was decreased in hDPSCs after LPS treatment. Rescue experiments showed that GIT2 could mediate the protective functions of circFKBP5 on hDPSCs under LPS treatment. Conclusions: CircFKBP5 could protect against LPS-induced apoptosis, inflammation, and osteogenic differentiation inhibition in hDPSCs via the miR-708-5p/GIT2 axis.http://www.sciencedirect.com/science/article/pii/S0020653922001939CircFKBP5InflammationhDPSCsOsteogenic differentiationPulpitis |
| spellingShingle | Cuiwei Liang Wenmiao Li Qian Huang Qitao Wen CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation International Dental Journal CircFKBP5 Inflammation hDPSCs Osteogenic differentiation Pulpitis |
| title | CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation |
| title_full | CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation |
| title_fullStr | CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation |
| title_full_unstemmed | CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation |
| title_short | CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation |
| title_sort | circfkbp5 suppresses apoptosis and inflammation and promotes osteogenic differentiation |
| topic | CircFKBP5 Inflammation hDPSCs Osteogenic differentiation Pulpitis |
| url | http://www.sciencedirect.com/science/article/pii/S0020653922001939 |
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