Metabolic engineering of Pseudomonas putida for production of the natural sweetener 5‐ketofructose from fructose or sucrose by periplasmic oxidation with a heterologous fructose dehydrogenase

Metabolic engineering of Pseudomonas putida for production of the natural sweetener 5‐ketofructose from fructose or sucrose by periplasmic oxidation with a heterologous fructose dehydrogenase

Summary 5‐Ketofructose (5‐KF) is a promising low‐calorie natural sweetener with the potential to reduce health problems caused by excessive sugar consumption. It is formed by periplasmic oxidation of fructose by fructose dehydrogenase (Fdh) of Gluconobacter japonicus, a membrane‐bound three‐subunit...

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Bibliographic Details
Main Authors:	Karen Wohlers, Astrid Wirtz, Alexander Reiter, Marco Oldiges, Meike Baumgart, Michael Bott
Format:	Article
Language:	English
Published:	Wiley 2021-11-01
Series:	Microbial Biotechnology
Online Access:	https://doi.org/10.1111/1751-7915.13913

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