Specific Urinary Metabolites in Malignant Melanoma
<i>Background and objectives:</i> Melanin, which has a confirmed role in melanoma cell behaviour, is formed in the process of melanogenesis and is synthesized from tryptophan, L-tyrosine and their metabolites. All these metabolites are easily detectable by chromatography in urine. <i&...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-05-01
|
| Series: | Medicina |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1010-660X/55/5/145 |
| _version_ | 1797727600917872640 |
|---|---|
| author | Marcela Valko-Rokytovská Beáta Hubková Anna Birková Jana Mašlanková Marek Stupák Marianna Zábavníková Beáta Čižmárová Mária Mareková |
| author_facet | Marcela Valko-Rokytovská Beáta Hubková Anna Birková Jana Mašlanková Marek Stupák Marianna Zábavníková Beáta Čižmárová Mária Mareková |
| author_sort | Marcela Valko-Rokytovská |
| collection | DOAJ |
| description | <i>Background and objectives:</i> Melanin, which has a confirmed role in melanoma cell behaviour, is formed in the process of melanogenesis and is synthesized from tryptophan, L-tyrosine and their metabolites. All these metabolites are easily detectable by chromatography in urine. <i>Materials and Methods:</i> Urine samples of 133 individuals (82 malignant melanoma patients and 51 healthy controls) were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC). The diagnosis of malignant melanoma was confirmed histologically. <i>Results:</i> Chromatograms of melanoma patients showed increased levels of 5,6-dihydroxyindole-2-carboxylic acid, vanilmandelic acid, homovanilic acid, tryptophan, 5-hydroxyindole-3-acetic acid, and indoxyl sulphate compared to healthy controls. Concentration of indoxyl sulphate, homovanilic acid and tryptophan were significantly increased even in the low clinical stage 0 of the disease (indoxyl sulphate, homovanilic acid and tryptophan in patients with clinical stage 0 vs. controls expressed as medium/ interquartile range in µmol/mmol creatinine: 28.37/15.30 vs. 5.00/6.91; 47.97/33.08 vs. 7.33/21.25; and 16.38/15.98 vs. 3.46/6.22, respectively). <i>Conclusions:</i> HPLC detection of metabolites of L-tyrosine and tryptophan in the urine of melanoma patients may play a significant role in diagnostics as well as a therapeutic strategy of melanoma cancer. |
| first_indexed | 2024-03-12T11:02:01Z |
| format | Article |
| id | doaj.art-f597d6f7bc514deb846556bd61180821 |
| institution | Directory Open Access Journal |
| issn | 1010-660X |
| language | English |
| last_indexed | 2024-03-12T11:02:01Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-f597d6f7bc514deb846556bd611808212023-09-02T05:30:55ZengMDPI AGMedicina1010-660X2019-05-0155514510.3390/medicina55050145medicina55050145Specific Urinary Metabolites in Malignant MelanomaMarcela Valko-Rokytovská0Beáta Hubková1Anna Birková2Jana Mašlanková3Marek Stupák4Marianna Zábavníková5Beáta Čižmárová6Mária Mareková7Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Tr. SNP 1, 040 11 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Tr. SNP 1, 040 11 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Tr. SNP 1, 040 11 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Tr. SNP 1, 040 11 Košice, SlovakiaKOREKTCHIR s.r.o., Poliklinika Terasa, Toryská 1, 041 90 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Tr. SNP 1, 040 11 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Tr. SNP 1, 040 11 Košice, Slovakia<i>Background and objectives:</i> Melanin, which has a confirmed role in melanoma cell behaviour, is formed in the process of melanogenesis and is synthesized from tryptophan, L-tyrosine and their metabolites. All these metabolites are easily detectable by chromatography in urine. <i>Materials and Methods:</i> Urine samples of 133 individuals (82 malignant melanoma patients and 51 healthy controls) were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC). The diagnosis of malignant melanoma was confirmed histologically. <i>Results:</i> Chromatograms of melanoma patients showed increased levels of 5,6-dihydroxyindole-2-carboxylic acid, vanilmandelic acid, homovanilic acid, tryptophan, 5-hydroxyindole-3-acetic acid, and indoxyl sulphate compared to healthy controls. Concentration of indoxyl sulphate, homovanilic acid and tryptophan were significantly increased even in the low clinical stage 0 of the disease (indoxyl sulphate, homovanilic acid and tryptophan in patients with clinical stage 0 vs. controls expressed as medium/ interquartile range in µmol/mmol creatinine: 28.37/15.30 vs. 5.00/6.91; 47.97/33.08 vs. 7.33/21.25; and 16.38/15.98 vs. 3.46/6.22, respectively). <i>Conclusions:</i> HPLC detection of metabolites of L-tyrosine and tryptophan in the urine of melanoma patients may play a significant role in diagnostics as well as a therapeutic strategy of melanoma cancer.https://www.mdpi.com/1010-660X/55/5/145HPLCurinemalignant melanomametabolite5,6-dihydroxyindole-2-carboxylic acidvanilmandelic acidhomovanilic acidtryptophan5-hydroxyindole-3-acetic acidindoxyl sulphate |
| spellingShingle | Marcela Valko-Rokytovská Beáta Hubková Anna Birková Jana Mašlanková Marek Stupák Marianna Zábavníková Beáta Čižmárová Mária Mareková Specific Urinary Metabolites in Malignant Melanoma Medicina HPLC urine malignant melanoma metabolite 5,6-dihydroxyindole-2-carboxylic acid vanilmandelic acid homovanilic acid tryptophan 5-hydroxyindole-3-acetic acid indoxyl sulphate |
| title | Specific Urinary Metabolites in Malignant Melanoma |
| title_full | Specific Urinary Metabolites in Malignant Melanoma |
| title_fullStr | Specific Urinary Metabolites in Malignant Melanoma |
| title_full_unstemmed | Specific Urinary Metabolites in Malignant Melanoma |
| title_short | Specific Urinary Metabolites in Malignant Melanoma |
| title_sort | specific urinary metabolites in malignant melanoma |
| topic | HPLC urine malignant melanoma metabolite 5,6-dihydroxyindole-2-carboxylic acid vanilmandelic acid homovanilic acid tryptophan 5-hydroxyindole-3-acetic acid indoxyl sulphate |
| url | https://www.mdpi.com/1010-660X/55/5/145 |
| work_keys_str_mv | AT marcelavalkorokytovska specificurinarymetabolitesinmalignantmelanoma AT beatahubkova specificurinarymetabolitesinmalignantmelanoma AT annabirkova specificurinarymetabolitesinmalignantmelanoma AT janamaslankova specificurinarymetabolitesinmalignantmelanoma AT marekstupak specificurinarymetabolitesinmalignantmelanoma AT mariannazabavnikova specificurinarymetabolitesinmalignantmelanoma AT beatacizmarova specificurinarymetabolitesinmalignantmelanoma AT mariamarekova specificurinarymetabolitesinmalignantmelanoma |