Towards Biological Control of <i>Aspergillus carbonarius</i> and <i>Botrytis cinerea</i> in Grapevine Berries and Transcriptomic Changes of Genes Encoding Pathogenesis-Related (PR) Proteins
Grapevine bunch rot, caused by <i>Botrytis cinerea</i> and <i>Aspergillus carbonarius</i>, causes important economic losses every year in grape production. In the present study, we examined the plant protective activity of the biological control agents, <i>Paenibacillus...
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MDPI AG
2021-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/5/970 |
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| author | Danai Gkizi Eirini G. Poulaki Sotirios E. Tjamos |
| author_facet | Danai Gkizi Eirini G. Poulaki Sotirios E. Tjamos |
| author_sort | Danai Gkizi |
| collection | DOAJ |
| description | Grapevine bunch rot, caused by <i>Botrytis cinerea</i> and <i>Aspergillus carbonarius</i>, causes important economic losses every year in grape production. In the present study, we examined the plant protective activity of the biological control agents, <i>Paenibacillus alvei</i> K165, <i>Blastobotrys</i> sp. FP12 and <i>Arthrobacter</i> sp. FP15 against <i>B. cinerea</i> and <i>A. carbonarius</i> on grapes. The in vitro experiments showed that strain K165 significantly reduced the growth of both fungi, while FP15 restricted the growth of <i>A. carbonarius</i> and FP12 was ineffective. Following the in vitro experiments, we conducted in planta experiments on grape berries. It was shown that K165, FP12 and FP15 reduced <i>A. carbonarius</i> rot severity by 81%, 57% and 37%, respectively, compared to the control, whereas, in the case of <i>B. cinerea</i>, the only protective treatment was that with K165, which reduced rot by 75%. The transcriptomic analysis of the genes encoding the pathogenesis-related proteins PR2, PR3, PR4 and PR5 indicates the activation of multiple defense responses involved in the biocontrol activity of the examined biocontrol agents. |
| first_indexed | 2024-03-10T11:28:18Z |
| format | Article |
| id | doaj.art-f59ca83cd37240e18c65348e26ccb849 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T11:28:18Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-f59ca83cd37240e18c65348e26ccb8492023-11-21T19:31:27ZengMDPI AGPlants2223-77472021-05-0110597010.3390/plants10050970Towards Biological Control of <i>Aspergillus carbonarius</i> and <i>Botrytis cinerea</i> in Grapevine Berries and Transcriptomic Changes of Genes Encoding Pathogenesis-Related (PR) ProteinsDanai Gkizi0Eirini G. Poulaki1Sotirios E. Tjamos2Laboratory of Plant Pathology, Agricultural University of Athens, 75 Iera Odos Str., 11855 Athens, GreeceLaboratory of Plant Pathology, Agricultural University of Athens, 75 Iera Odos Str., 11855 Athens, GreeceLaboratory of Plant Pathology, Agricultural University of Athens, 75 Iera Odos Str., 11855 Athens, GreeceGrapevine bunch rot, caused by <i>Botrytis cinerea</i> and <i>Aspergillus carbonarius</i>, causes important economic losses every year in grape production. In the present study, we examined the plant protective activity of the biological control agents, <i>Paenibacillus alvei</i> K165, <i>Blastobotrys</i> sp. FP12 and <i>Arthrobacter</i> sp. FP15 against <i>B. cinerea</i> and <i>A. carbonarius</i> on grapes. The in vitro experiments showed that strain K165 significantly reduced the growth of both fungi, while FP15 restricted the growth of <i>A. carbonarius</i> and FP12 was ineffective. Following the in vitro experiments, we conducted in planta experiments on grape berries. It was shown that K165, FP12 and FP15 reduced <i>A. carbonarius</i> rot severity by 81%, 57% and 37%, respectively, compared to the control, whereas, in the case of <i>B. cinerea</i>, the only protective treatment was that with K165, which reduced rot by 75%. The transcriptomic analysis of the genes encoding the pathogenesis-related proteins PR2, PR3, PR4 and PR5 indicates the activation of multiple defense responses involved in the biocontrol activity of the examined biocontrol agents.https://www.mdpi.com/2223-7747/10/5/970<i>Arthrobacter</i> sp.<i>Blastobotrys</i> sp.grapevine bunch rotinduced systemic resistance<i>Paenibacillus alvei</i> |
| spellingShingle | Danai Gkizi Eirini G. Poulaki Sotirios E. Tjamos Towards Biological Control of <i>Aspergillus carbonarius</i> and <i>Botrytis cinerea</i> in Grapevine Berries and Transcriptomic Changes of Genes Encoding Pathogenesis-Related (PR) Proteins Plants <i>Arthrobacter</i> sp. <i>Blastobotrys</i> sp. grapevine bunch rot induced systemic resistance <i>Paenibacillus alvei</i> |
| title | Towards Biological Control of <i>Aspergillus carbonarius</i> and <i>Botrytis cinerea</i> in Grapevine Berries and Transcriptomic Changes of Genes Encoding Pathogenesis-Related (PR) Proteins |
| title_full | Towards Biological Control of <i>Aspergillus carbonarius</i> and <i>Botrytis cinerea</i> in Grapevine Berries and Transcriptomic Changes of Genes Encoding Pathogenesis-Related (PR) Proteins |
| title_fullStr | Towards Biological Control of <i>Aspergillus carbonarius</i> and <i>Botrytis cinerea</i> in Grapevine Berries and Transcriptomic Changes of Genes Encoding Pathogenesis-Related (PR) Proteins |
| title_full_unstemmed | Towards Biological Control of <i>Aspergillus carbonarius</i> and <i>Botrytis cinerea</i> in Grapevine Berries and Transcriptomic Changes of Genes Encoding Pathogenesis-Related (PR) Proteins |
| title_short | Towards Biological Control of <i>Aspergillus carbonarius</i> and <i>Botrytis cinerea</i> in Grapevine Berries and Transcriptomic Changes of Genes Encoding Pathogenesis-Related (PR) Proteins |
| title_sort | towards biological control of i aspergillus carbonarius i and i botrytis cinerea i in grapevine berries and transcriptomic changes of genes encoding pathogenesis related pr proteins |
| topic | <i>Arthrobacter</i> sp. <i>Blastobotrys</i> sp. grapevine bunch rot induced systemic resistance <i>Paenibacillus alvei</i> |
| url | https://www.mdpi.com/2223-7747/10/5/970 |
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