Effect of <i>Nardostachys jatamansi</i> DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats
The study aimed to investigate the protective action of jatamansi (<i>Nardostachys jatamansi</i> DC.) against doxorubicin cardiotoxicity. Methanolic extract of jatamansi (MEJ) was prepared and standardized using HPTLC fingerprinting, GC-MS chemoprofiling, total phenolic content, and anti...
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MDPI AG
2020-11-01
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| author | Mhaveer Singh Mohammad Ahmed Khan Kamal Y. T. Javed Ahmad Usama A. Fahmy Sabna Kotta Nabil A. Alhakamy Sayeed Ahmad |
| author_facet | Mhaveer Singh Mohammad Ahmed Khan Kamal Y. T. Javed Ahmad Usama A. Fahmy Sabna Kotta Nabil A. Alhakamy Sayeed Ahmad |
| author_sort | Mhaveer Singh |
| collection | DOAJ |
| description | The study aimed to investigate the protective action of jatamansi (<i>Nardostachys jatamansi</i> DC.) against doxorubicin cardiotoxicity. Methanolic extract of jatamansi (MEJ) was prepared and standardized using HPTLC fingerprinting, GC-MS chemoprofiling, total phenolic content, and antioxidant activity in vitro. Further in vivo activity was evaluated using rodent model. Animals were divided into five groups (<i>n</i> = 6) namely control (CNT) (Normal saline), toxicant (TOX, without any treatment), MEJ at low dose (JAT1), MEJ at high dose (JAT2), and standard desferrioxamine (STD). All groups except control received doxorubicin 2.5 mg per Kg intra-peritoneally for 3 weeks in twice a week regimen. After 3 weeks, the blood samples and cardiac tissues were collected from all groups for biochemical and histopathological evaluation. Treatment with MEJ at both dose levels exhibited significant reduction (<i>p</i> < 0.001 vs. toxicant) of serum CK-MB (heart creatine kinase), LDH (Lactate dehydrogenase) & HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) levels, and tissue MDA (melondialdehyde) level; insignificant difference was observed (<i>p</i> > 0.05) in TNF-alpha (tumour necrosis factor), IL-6 (interleukine-6) levels and caspase activity as compared to TOX. Histopathological evaluation of cardiac tissues of different treatment groups further reinforced the findings of biochemical estimation. This study concludes that jatamansi can protect cardiac tissues from oxidative stress-induced cell injury and lipid peroxidation as well as against inflammatory and apoptotic effects on cardiac tissues. |
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| spelling | doaj.art-f5a2e95513524d03aaa9fdcb9904ef552023-11-20T21:03:22ZengMDPI AGPlants2223-77472020-11-01911157910.3390/plants9111579Effect of <i>Nardostachys jatamansi</i> DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar RatsMhaveer Singh0Mohammad Ahmed Khan1Kamal Y. T.2Javed Ahmad3Usama A. Fahmy4Sabna Kotta5Nabil A. Alhakamy6Sayeed Ahmad7School of Medical and Allied Sciences, G D Goenka University, Haryana 122103, IndiaDepartment of Pharmacology, School of Pharmaceutical Education & Research, Hamdard University, Hamdard Nagar, New Delhi 110062, IndiaDepartment of Pharmacognosy, College of Pharmacy, King Khalid University, Abha 611441, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Najran University, Najran 11001, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaBioactive Natural Product Laboratory, School of Pharmaceutical Education & Research, Hamdard University, Hamdard Nagar, New Delhi 110062, IndiaThe study aimed to investigate the protective action of jatamansi (<i>Nardostachys jatamansi</i> DC.) against doxorubicin cardiotoxicity. Methanolic extract of jatamansi (MEJ) was prepared and standardized using HPTLC fingerprinting, GC-MS chemoprofiling, total phenolic content, and antioxidant activity in vitro. Further in vivo activity was evaluated using rodent model. Animals were divided into five groups (<i>n</i> = 6) namely control (CNT) (Normal saline), toxicant (TOX, without any treatment), MEJ at low dose (JAT1), MEJ at high dose (JAT2), and standard desferrioxamine (STD). All groups except control received doxorubicin 2.5 mg per Kg intra-peritoneally for 3 weeks in twice a week regimen. After 3 weeks, the blood samples and cardiac tissues were collected from all groups for biochemical and histopathological evaluation. Treatment with MEJ at both dose levels exhibited significant reduction (<i>p</i> < 0.001 vs. toxicant) of serum CK-MB (heart creatine kinase), LDH (Lactate dehydrogenase) & HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) levels, and tissue MDA (melondialdehyde) level; insignificant difference was observed (<i>p</i> > 0.05) in TNF-alpha (tumour necrosis factor), IL-6 (interleukine-6) levels and caspase activity as compared to TOX. Histopathological evaluation of cardiac tissues of different treatment groups further reinforced the findings of biochemical estimation. This study concludes that jatamansi can protect cardiac tissues from oxidative stress-induced cell injury and lipid peroxidation as well as against inflammatory and apoptotic effects on cardiac tissues.https://www.mdpi.com/2223-7747/9/11/1579biochemical estimationcardioprotectioncardiotoxicitydoxorubicinlipid peroxidation<i>Nardostachys jatamansi</i> DC. |
| spellingShingle | Mhaveer Singh Mohammad Ahmed Khan Kamal Y. T. Javed Ahmad Usama A. Fahmy Sabna Kotta Nabil A. Alhakamy Sayeed Ahmad Effect of <i>Nardostachys jatamansi</i> DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats Plants biochemical estimation cardioprotection cardiotoxicity doxorubicin lipid peroxidation <i>Nardostachys jatamansi</i> DC. |
| title | Effect of <i>Nardostachys jatamansi</i> DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats |
| title_full | Effect of <i>Nardostachys jatamansi</i> DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats |
| title_fullStr | Effect of <i>Nardostachys jatamansi</i> DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats |
| title_full_unstemmed | Effect of <i>Nardostachys jatamansi</i> DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats |
| title_short | Effect of <i>Nardostachys jatamansi</i> DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats |
| title_sort | effect of i nardostachys jatamansi i dc on apoptosis inflammation and oxidative stress induced by doxorubicin in wistar rats |
| topic | biochemical estimation cardioprotection cardiotoxicity doxorubicin lipid peroxidation <i>Nardostachys jatamansi</i> DC. |
| url | https://www.mdpi.com/2223-7747/9/11/1579 |
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