Can Salivary Acetylcholinesterase be a Diagnostic Biomarker for Alzheimer?
Introduction: The loss of brain cholinergic activity is a key phenomenon in the biochemistry of Alzheimer's Disease (AD). Due to the specific biosynthesis of Acetylcholinesterase (AChE) of cholinergic neurons, the enzyme has been proposed as a potential biochemical marker of cholinergic act...
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JCDR Research and Publications Private Limited
2017-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/9192/21715_F(DK)_PF1(DK_NE)_PFA(DK)_PF2(NE_DK).pdf |
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| author | Sedigheh Bakhtiari Nahid Beladi Moghadam Marjan Ehsani Hamed Mortazavi Siamak Sabour Mahin Bakhshi |
| author_facet | Sedigheh Bakhtiari Nahid Beladi Moghadam Marjan Ehsani Hamed Mortazavi Siamak Sabour Mahin Bakhshi |
| author_sort | Sedigheh Bakhtiari |
| collection | DOAJ |
| description | Introduction: The loss of brain cholinergic activity is a key
phenomenon in the biochemistry of Alzheimer's Disease (AD).
Due to the specific biosynthesis of Acetylcholinesterase (AChE) of
cholinergic neurons, the enzyme has been proposed as a potential
biochemical marker of cholinergic activity. AChE is expressed not
only in the Central Nervous System (CNS), Peripheral Nervous
System (PNS) and muscles, but also on the surface of blood cells
and saliva.
Aim: This study aimed to measure salivary AChE activity in AD
and to determine the feasibility of creating a simple laboratory
test for diagnosing such patients.
Materials and Methods: In this cross-sectional study, the
recorded data were obtained from 15 Alzheimer’s patients on
memantine therapy and 15 healthy subjects. Unstimulated
whole saliva samples were collected from the participants and
salivary levels of AChE activity were determined by using the
Ellman colorimetric method. The Mann Whitney U test was used
to compare the average (median) of AChE activity between AD
and controls. In order to adjust for possible confounding factors,
partial correlation coefficient and multivariate linear regressions
were used.
Results: Although the average of AChE activity in the saliva
of people with AD was lower compared to the control group,
we found no statistically significant differences using Mann
Whitney U test (138 in control group vs. 175 in Alzheimer's
patients, p value=0.25). Additionally, no significant differences
were observed in the activity of this enzyme in both sexes or
with increased age or duration of the disease. After adjusting
for age and gender, there was no association between AChE
activity and AD (regression coefficient β=0.08; p value= 0.67).
Conclusion: Saliva AChE activity was not significantly associated with AD. This study might help in introduce a new diagnostic aid for AD or monitor patients with AD. |
| first_indexed | 2024-12-21T18:42:35Z |
| format | Article |
| id | doaj.art-f5a7c3262e7848dc83d1f0bb92f83e8d |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-21T18:42:35Z |
| publishDate | 2017-01-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-f5a7c3262e7848dc83d1f0bb92f83e8d2022-12-21T18:53:58ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-01-01111ZC58ZC6010.7860/JCDR/2017/21715.9192Can Salivary Acetylcholinesterase be a Diagnostic Biomarker for Alzheimer?Sedigheh Bakhtiari0Nahid Beladi Moghadam1Marjan Ehsani2Hamed Mortazavi3Siamak Sabour4Mahin Bakhshi5Associate Professor, Department of Oral Medicine, Dental Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Assistant Professor, Department of Neurology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.General Practioner, Dental Faculty, Shahid Beheshti University of Medical Science, Tehran, Iran.Associate Professor, Department of Oral Medicine, Dental Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Associate Professor, Department of Clinical Epidemiology, School of Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Associate Professor, Department of Oral Medicine, Dental Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Introduction: The loss of brain cholinergic activity is a key phenomenon in the biochemistry of Alzheimer's Disease (AD). Due to the specific biosynthesis of Acetylcholinesterase (AChE) of cholinergic neurons, the enzyme has been proposed as a potential biochemical marker of cholinergic activity. AChE is expressed not only in the Central Nervous System (CNS), Peripheral Nervous System (PNS) and muscles, but also on the surface of blood cells and saliva. Aim: This study aimed to measure salivary AChE activity in AD and to determine the feasibility of creating a simple laboratory test for diagnosing such patients. Materials and Methods: In this cross-sectional study, the recorded data were obtained from 15 Alzheimer’s patients on memantine therapy and 15 healthy subjects. Unstimulated whole saliva samples were collected from the participants and salivary levels of AChE activity were determined by using the Ellman colorimetric method. The Mann Whitney U test was used to compare the average (median) of AChE activity between AD and controls. In order to adjust for possible confounding factors, partial correlation coefficient and multivariate linear regressions were used. Results: Although the average of AChE activity in the saliva of people with AD was lower compared to the control group, we found no statistically significant differences using Mann Whitney U test (138 in control group vs. 175 in Alzheimer's patients, p value=0.25). Additionally, no significant differences were observed in the activity of this enzyme in both sexes or with increased age or duration of the disease. After adjusting for age and gender, there was no association between AChE activity and AD (regression coefficient β=0.08; p value= 0.67). Conclusion: Saliva AChE activity was not significantly associated with AD. This study might help in introduce a new diagnostic aid for AD or monitor patients with AD.https://jcdr.net/articles/PDF/9192/21715_F(DK)_PF1(DK_NE)_PFA(DK)_PF2(NE_DK).pdfcholinesterasedementiaexploringfluid biomarker |
| spellingShingle | Sedigheh Bakhtiari Nahid Beladi Moghadam Marjan Ehsani Hamed Mortazavi Siamak Sabour Mahin Bakhshi Can Salivary Acetylcholinesterase be a Diagnostic Biomarker for Alzheimer? Journal of Clinical and Diagnostic Research cholinesterase dementia exploring fluid biomarker |
| title | Can Salivary Acetylcholinesterase be a Diagnostic Biomarker for Alzheimer? |
| title_full | Can Salivary Acetylcholinesterase be a Diagnostic Biomarker for Alzheimer? |
| title_fullStr | Can Salivary Acetylcholinesterase be a Diagnostic Biomarker for Alzheimer? |
| title_full_unstemmed | Can Salivary Acetylcholinesterase be a Diagnostic Biomarker for Alzheimer? |
| title_short | Can Salivary Acetylcholinesterase be a Diagnostic Biomarker for Alzheimer? |
| title_sort | can salivary acetylcholinesterase be a diagnostic biomarker for alzheimer |
| topic | cholinesterase dementia exploring fluid biomarker |
| url | https://jcdr.net/articles/PDF/9192/21715_F(DK)_PF1(DK_NE)_PFA(DK)_PF2(NE_DK).pdf |
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