Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant
Strategic implementation of vermicompost as safe biofertilizer besides defensing saline soils offer dual function solving problems in developing countries. The current study aims to utilize vermicompost (VC) for amelioration of 200mM NaCl in <i>Vicia faba</i> Aspani cultivar and investig...
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MDPI AG
2021-11-01
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| Series: | Plants |
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| author | Rehab El-Dakak Weam El-Aggan Ghadah Badr Amira Helaly Amel Tammam |
| author_facet | Rehab El-Dakak Weam El-Aggan Ghadah Badr Amira Helaly Amel Tammam |
| author_sort | Rehab El-Dakak |
| collection | DOAJ |
| description | Strategic implementation of vermicompost as safe biofertilizer besides defensing saline soils offer dual function solving problems in developing countries. The current study aims to utilize vermicompost (VC) for amelioration of 200mM NaCl in <i>Vicia faba</i> Aspani cultivar and investigate the molecular role of salt overly sensitive pathway (SOS1). The experiment was conducted following a completely randomized design with three replicates. Treatments include 0; 2.5; 5; 10; 15% dried VC intermingled with soil mixture (clay: sand; 1:2) and/or 200 mM NaCl. The results show that salinity stress decreased broad bean fresh and dry weight; and K<sup>+</sup>/Na<sup>+</sup>. However, malonedialdehyde and H<sub>2</sub>O<sub>2</sub> contents; increased. Application of 10% VC and salinity stress increases Ca<sup>2+</sup> (41% and 50%), K<sup>+</sup>/Na<sup>+</sup> (125% and 89%), Mg<sup>2+</sup> (25% and 36%), N (8% and 11%), indole acetic acid (70% and 152%) and proteins (9% and 13%) for root and shoot, respectively, in comparison to salt treated pots. Moreover, all examined enzymatic antioxidants and their substrates increased, except glutathione reductase. A parallel decrease in abscisic acid (75% and 29%) and proline (59% and 58%) was also recorded for roots and leaves, respectively. Interestingly, the highly significant increase in gene expression of SOS1 (45-fold) could drive defense machinery of broad bean to counteract 200 mM NaCl. |
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| language | English |
| last_indexed | 2024-03-10T05:08:04Z |
| publishDate | 2021-11-01 |
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| series | Plants |
| spelling | doaj.art-f5aedb5051574562bcf23a457847af1b2023-11-23T01:06:24ZengMDPI AGPlants2223-77472021-11-011011247710.3390/plants10112477Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> PlantRehab El-Dakak0Weam El-Aggan1Ghadah Badr2Amira Helaly3Amel Tammam4Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria 21511, EgyptDepartment of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria 21511, EgyptDepartment of Biological Science, Faculty of Science, Elmergib University, Al Khums P.O. Box 40414, LibyaDepartment of Vegetable Crops, Faculty of Agriculture, Alexandria University, Alexandria 21545, EgyptDepartment of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria 21511, EgyptStrategic implementation of vermicompost as safe biofertilizer besides defensing saline soils offer dual function solving problems in developing countries. The current study aims to utilize vermicompost (VC) for amelioration of 200mM NaCl in <i>Vicia faba</i> Aspani cultivar and investigate the molecular role of salt overly sensitive pathway (SOS1). The experiment was conducted following a completely randomized design with three replicates. Treatments include 0; 2.5; 5; 10; 15% dried VC intermingled with soil mixture (clay: sand; 1:2) and/or 200 mM NaCl. The results show that salinity stress decreased broad bean fresh and dry weight; and K<sup>+</sup>/Na<sup>+</sup>. However, malonedialdehyde and H<sub>2</sub>O<sub>2</sub> contents; increased. Application of 10% VC and salinity stress increases Ca<sup>2+</sup> (41% and 50%), K<sup>+</sup>/Na<sup>+</sup> (125% and 89%), Mg<sup>2+</sup> (25% and 36%), N (8% and 11%), indole acetic acid (70% and 152%) and proteins (9% and 13%) for root and shoot, respectively, in comparison to salt treated pots. Moreover, all examined enzymatic antioxidants and their substrates increased, except glutathione reductase. A parallel decrease in abscisic acid (75% and 29%) and proline (59% and 58%) was also recorded for roots and leaves, respectively. Interestingly, the highly significant increase in gene expression of SOS1 (45-fold) could drive defense machinery of broad bean to counteract 200 mM NaCl.https://www.mdpi.com/2223-7747/10/11/2477<i>Vicia</i> <i>faba</i>broad beansalinitySOS1stressvermicompost |
| spellingShingle | Rehab El-Dakak Weam El-Aggan Ghadah Badr Amira Helaly Amel Tammam Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant Plants <i>Vicia</i> <i>faba</i> broad bean salinity SOS1 stress vermicompost |
| title | Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant |
| title_full | Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant |
| title_fullStr | Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant |
| title_full_unstemmed | Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant |
| title_short | Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant |
| title_sort | positive salt tolerance modulation via vermicompost regulation of sos1 gene expression and antioxidant homeostasis in i vicia i i faba i plant |
| topic | <i>Vicia</i> <i>faba</i> broad bean salinity SOS1 stress vermicompost |
| url | https://www.mdpi.com/2223-7747/10/11/2477 |
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