Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (<it>Oryza sativa </it>L.)
<p>Abstract</p> <p>Background</p> <p>Many plant β-galactosidases (Bgals) have been well characterized and their deduced biological functions mainly involve degradation of structural pectins, xyloglucans or arabinogalactoproteins in plant cell walls. However, gene multip...
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| Format: | Article |
| Language: | English |
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BMC
2008-07-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/8/84 |
| _version_ | 1811281567818973184 |
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| author | Maneesan Janjira Chantarangsee Mallika Tanthanuch Waraporn Ketudat-Cairns James |
| author_facet | Maneesan Janjira Chantarangsee Mallika Tanthanuch Waraporn Ketudat-Cairns James |
| author_sort | Maneesan Janjira |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Many plant β-galactosidases (Bgals) have been well characterized and their deduced biological functions mainly involve degradation of structural pectins, xyloglucans or arabinogalactoproteins in plant cell walls. However, gene multiplicity in glycosyl hydrolase family 35 (GH35), to which these proteins belong, implies diverse functions. In this study, the gene multiplicity, apparent evolutionary relationships and transcript expression of rice Bgal genes were examined, in order to predict their biological functions.</p> <p>Results</p> <p>Fifteen rice Bgal genes were identified in the plant genome, one of which encodes a protein similar to animal Bgals (<it>OsBgal9</it>), and the remaining 14 fall in a nearly plant-specific subfamily of Bgals. The presence of both classes of Bgals in bryophytes, as well as vascular plants, suggests both gene lineages were present early in plant evolution. All 15 proteins were predicted to contain secretory signal sequences, suggesting they have secretory pathway or external roles. RT-PCR and database analysis found two distinct lineages to be expressed nearly exclusively in reproductive tissues and to be closely related to <it>Arabidopsis </it>Bgals expressed most highly in flower and pollen. On the other hand, <it>OsBgal6 </it>is expressed primarily in young vegetative tissues, and alternative splicing in panicle prevents its production of full-length protein in this reproductive tissue. <it>OsBgal11 </it>also showed alternative splicing to produce different length proteins. OsBgal13 produced by recombinant expression in <it>Escherichia coli </it>hydrolyzed α-L-arabinoside in addition to β-D-galactoside and β-(1→3)-, β-(1→4)- and β-(1→6)- linked galacto-oligosaccharides.</p> <p>Conclusion</p> <p>Rice <it>GH35 </it>contains fifteen genes with a diversity of protein sequences, predicted locations and expression and splicing patterns that suggest that OsBgals enzymes may play a variety of roles in metabolism of cell wall polysaccharides, glycoproteins and glycolipids.</p> |
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| format | Article |
| id | doaj.art-f5af4f29b4c740ecb93c7d7a2fbc7561 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T01:35:57Z |
| publishDate | 2008-07-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-f5af4f29b4c740ecb93c7d7a2fbc75612022-12-22T03:08:24ZengBMCBMC Plant Biology1471-22292008-07-01818410.1186/1471-2229-8-84Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (<it>Oryza sativa </it>L.)Maneesan JanjiraChantarangsee MallikaTanthanuch WarapornKetudat-Cairns James<p>Abstract</p> <p>Background</p> <p>Many plant β-galactosidases (Bgals) have been well characterized and their deduced biological functions mainly involve degradation of structural pectins, xyloglucans or arabinogalactoproteins in plant cell walls. However, gene multiplicity in glycosyl hydrolase family 35 (GH35), to which these proteins belong, implies diverse functions. In this study, the gene multiplicity, apparent evolutionary relationships and transcript expression of rice Bgal genes were examined, in order to predict their biological functions.</p> <p>Results</p> <p>Fifteen rice Bgal genes were identified in the plant genome, one of which encodes a protein similar to animal Bgals (<it>OsBgal9</it>), and the remaining 14 fall in a nearly plant-specific subfamily of Bgals. The presence of both classes of Bgals in bryophytes, as well as vascular plants, suggests both gene lineages were present early in plant evolution. All 15 proteins were predicted to contain secretory signal sequences, suggesting they have secretory pathway or external roles. RT-PCR and database analysis found two distinct lineages to be expressed nearly exclusively in reproductive tissues and to be closely related to <it>Arabidopsis </it>Bgals expressed most highly in flower and pollen. On the other hand, <it>OsBgal6 </it>is expressed primarily in young vegetative tissues, and alternative splicing in panicle prevents its production of full-length protein in this reproductive tissue. <it>OsBgal11 </it>also showed alternative splicing to produce different length proteins. OsBgal13 produced by recombinant expression in <it>Escherichia coli </it>hydrolyzed α-L-arabinoside in addition to β-D-galactoside and β-(1→3)-, β-(1→4)- and β-(1→6)- linked galacto-oligosaccharides.</p> <p>Conclusion</p> <p>Rice <it>GH35 </it>contains fifteen genes with a diversity of protein sequences, predicted locations and expression and splicing patterns that suggest that OsBgals enzymes may play a variety of roles in metabolism of cell wall polysaccharides, glycoproteins and glycolipids.</p>http://www.biomedcentral.com/1471-2229/8/84 |
| spellingShingle | Maneesan Janjira Chantarangsee Mallika Tanthanuch Waraporn Ketudat-Cairns James Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (<it>Oryza sativa </it>L.) BMC Plant Biology |
| title | Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (<it>Oryza sativa </it>L.) |
| title_full | Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (<it>Oryza sativa </it>L.) |
| title_fullStr | Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (<it>Oryza sativa </it>L.) |
| title_full_unstemmed | Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (<it>Oryza sativa </it>L.) |
| title_short | Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (<it>Oryza sativa </it>L.) |
| title_sort | genomic and expression analysis of glycosyl hydrolase family 35 genes from rice it oryza sativa it l |
| url | http://www.biomedcentral.com/1471-2229/8/84 |
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