Amplification of a transgene within a long array of replication origins favors higher gene expression in animal cells.

Amplification of a transgene within a long array of replication origins favors higher gene expression in animal cells.

Plasmids with both a mammalian replication initiation region (IR) and a matrix attachment region (MAR) are spontaneously amplified in transfected cells, and generate extrachromosomal double minute (DM) or chromosomal homogeneously staining region (HSR). We previously isolated the shortest core IR (G...

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Bibliographic Details
Main Authors: Kiwamu Ohsaki, Yusuke Ohgaki, Noriaki Shimizu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5389822?pdf=render

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