Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray<sup>®</sup> Multiplex-PCR Technology
The objectives of this study were (a) to detect gastrointestinal pathogens in faecal samples of sheep and goats using the FilmArray<sup>®</sup> GI Panel and (b) to evaluate factors that were associated with their presence. Faecal samples from ewes or does in 70 sheep flocks and 24 goat h...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/714 |
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| author | Katerina Tsilipounidaki Zoe Florou Daphne T. Lianou Charalambia K. Michael Eleni I. Katsarou Anargyros Skoulakis George C. Fthenakis Efthymia Petinaki |
| author_facet | Katerina Tsilipounidaki Zoe Florou Daphne T. Lianou Charalambia K. Michael Eleni I. Katsarou Anargyros Skoulakis George C. Fthenakis Efthymia Petinaki |
| author_sort | Katerina Tsilipounidaki |
| collection | DOAJ |
| description | The objectives of this study were (a) to detect gastrointestinal pathogens in faecal samples of sheep and goats using the FilmArray<sup>®</sup> GI Panel and (b) to evaluate factors that were associated with their presence. Faecal samples from ewes or does in 70 sheep flocks and 24 goat herds in Greece were tested for the presence of 22 gastrointestinal pathogens by means of the BioFire<sup>®</sup> FilmArray<sup>®</sup> Gastrointestinal (GI) Panel. The most frequently detected pathogens were Shiga-like toxin-producing <i>Escherichia coli</i> stx1/stx2 (94.7% of farms), <i>Giardia lamblia</i> (59.6%), and <i>Campylobacter</i> spp. (50.0% of farms). Other pathogens detected were <i>Cryptosporidium</i> spp., <i>Salmonella</i> spp., enterotoxigenic <i>E. coli</i> lt/st, <i>Yersinia enterocolitica</i>, <i>E. coli</i> O157, Rotavirus A, <i>Shigella</i>/enteroinvasive <i>E. coli,</i> and <i>Plesiomonas</i><i>shigelloides</i>. There was a difference in the prevalence of detection of pathogens between sheep and goat farms only for <i>Salmonella</i> spp.: 18.3% versus 0.0%, respectively. Mixed infections were detected in 76 farms (80.9% of farms), specifically 57 sheep flocks and 19 goat herds, with on average, 2.5 ± 0.1 pathogens detected per farm. The body condition score of ewes in farms, in which only one pathogen was detected in faecal samples, was significantly higher than that of ewes in farms, in which at least two pathogens were detected: 2.55 ± 0.11 versus 2.31 ± 0.04. In sheep flocks, the number of pathogens in faecal samples was significantly higher in farms with semi-extensive management. In goat herds, the number of pathogens in faecal samples was positively correlated with average precipitation and inversely correlated with temperature range in the respective locations. |
| first_indexed | 2024-03-09T04:23:27Z |
| format | Article |
| id | doaj.art-f5b16ed4d74d4d26b04844caadd57899 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T04:23:27Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f5b16ed4d74d4d26b04844caadd578992023-12-03T13:44:48ZengMDPI AGMicroorganisms2076-26072022-03-0110471410.3390/microorganisms10040714Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray<sup>®</sup> Multiplex-PCR TechnologyKaterina Tsilipounidaki0Zoe Florou1Daphne T. Lianou2Charalambia K. Michael3Eleni I. Katsarou4Anargyros Skoulakis5George C. Fthenakis6Efthymia Petinaki7University Hospital of Larissa, 41110 Larissa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceThe objectives of this study were (a) to detect gastrointestinal pathogens in faecal samples of sheep and goats using the FilmArray<sup>®</sup> GI Panel and (b) to evaluate factors that were associated with their presence. Faecal samples from ewes or does in 70 sheep flocks and 24 goat herds in Greece were tested for the presence of 22 gastrointestinal pathogens by means of the BioFire<sup>®</sup> FilmArray<sup>®</sup> Gastrointestinal (GI) Panel. The most frequently detected pathogens were Shiga-like toxin-producing <i>Escherichia coli</i> stx1/stx2 (94.7% of farms), <i>Giardia lamblia</i> (59.6%), and <i>Campylobacter</i> spp. (50.0% of farms). Other pathogens detected were <i>Cryptosporidium</i> spp., <i>Salmonella</i> spp., enterotoxigenic <i>E. coli</i> lt/st, <i>Yersinia enterocolitica</i>, <i>E. coli</i> O157, Rotavirus A, <i>Shigella</i>/enteroinvasive <i>E. coli,</i> and <i>Plesiomonas</i><i>shigelloides</i>. There was a difference in the prevalence of detection of pathogens between sheep and goat farms only for <i>Salmonella</i> spp.: 18.3% versus 0.0%, respectively. Mixed infections were detected in 76 farms (80.9% of farms), specifically 57 sheep flocks and 19 goat herds, with on average, 2.5 ± 0.1 pathogens detected per farm. The body condition score of ewes in farms, in which only one pathogen was detected in faecal samples, was significantly higher than that of ewes in farms, in which at least two pathogens were detected: 2.55 ± 0.11 versus 2.31 ± 0.04. In sheep flocks, the number of pathogens in faecal samples was significantly higher in farms with semi-extensive management. In goat herds, the number of pathogens in faecal samples was positively correlated with average precipitation and inversely correlated with temperature range in the respective locations.https://www.mdpi.com/2076-2607/10/4/714<i>Campylobacter</i><i>Cryptosporidium</i>diarrhoea<i>Escherichia coli</i>FilmArray<i>Giardia</i> |
| spellingShingle | Katerina Tsilipounidaki Zoe Florou Daphne T. Lianou Charalambia K. Michael Eleni I. Katsarou Anargyros Skoulakis George C. Fthenakis Efthymia Petinaki Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray<sup>®</sup> Multiplex-PCR Technology Microorganisms <i>Campylobacter</i> <i>Cryptosporidium</i> diarrhoea <i>Escherichia coli</i> FilmArray <i>Giardia</i> |
| title | Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray<sup>®</sup> Multiplex-PCR Technology |
| title_full | Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray<sup>®</sup> Multiplex-PCR Technology |
| title_fullStr | Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray<sup>®</sup> Multiplex-PCR Technology |
| title_full_unstemmed | Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray<sup>®</sup> Multiplex-PCR Technology |
| title_short | Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray<sup>®</sup> Multiplex-PCR Technology |
| title_sort | detection of zoonotic gastrointestinal pathogens in dairy sheep and goats by using filmarray sup r sup multiplex pcr technology |
| topic | <i>Campylobacter</i> <i>Cryptosporidium</i> diarrhoea <i>Escherichia coli</i> FilmArray <i>Giardia</i> |
| url | https://www.mdpi.com/2076-2607/10/4/714 |
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