Analysis of Genetic Determination of <I>Vibrio cholerae</I> Tweenase Activity
Studied are Vibrio cholerae of different serogroups on the presence of cef (CHO cell elongating factor) gene and activity against tweens and tributyrin using HDS-agar, prepared on the basis of bakery yeast pancreatic digest. Determined is the fact that all cef-positive strains hydrolyze tweens 20, 4...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2012-08-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/870 |
| _version_ | 1797220978401476608 |
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| author | A. B. Mazrukho E. V. Monakhova O. V. Duvanova B. N. Mishan’Kin V. D. Kruglikov |
| author_facet | A. B. Mazrukho E. V. Monakhova O. V. Duvanova B. N. Mishan’Kin V. D. Kruglikov |
| author_sort | A. B. Mazrukho |
| collection | DOAJ |
| description | Studied are Vibrio cholerae of different serogroups on the presence of cef (CHO cell elongating factor) gene and activity against tweens and tributyrin using HDS-agar, prepared on the basis of bakery yeast pancreatic digest. Determined is the fact that all cef-positive strains hydrolyze tweens 20, 40, 60, 80, 85 and all but toxigenic V.cholerae O139, hydrolyze tributyrin. In contrast, non-toxigenic cef-negative strains of O139 serogroup, are active only against the latter. Apparently, the tweenase activity of Vibrio cholerae is provided, partly, by Cef, and the ability to hydrolyze tributyrin is the result of combined activity of Cef and other ferments. Shown is the efficiency of HDS-agar application to determine these characteristics. |
| first_indexed | 2024-03-08T13:43:59Z |
| format | Article |
| id | doaj.art-f5b1d843da264c7da20565fa35220352 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:58:07Z |
| publishDate | 2012-08-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-f5b1d843da264c7da20565fa352203522024-04-05T16:47:08ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2012-08-0104(114)444610.21055/0370-1069-2012-4-44-46854Analysis of Genetic Determination of <I>Vibrio cholerae</I> Tweenase ActivityA. B. Mazrukho0E. V. Monakhova1O. V. Duvanova2B. N. Mishan’Kin3V. D. Kruglikov4Rostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteStudied are Vibrio cholerae of different serogroups on the presence of cef (CHO cell elongating factor) gene and activity against tweens and tributyrin using HDS-agar, prepared on the basis of bakery yeast pancreatic digest. Determined is the fact that all cef-positive strains hydrolyze tweens 20, 40, 60, 80, 85 and all but toxigenic V.cholerae O139, hydrolyze tributyrin. In contrast, non-toxigenic cef-negative strains of O139 serogroup, are active only against the latter. Apparently, the tweenase activity of Vibrio cholerae is provided, partly, by Cef, and the ability to hydrolyze tributyrin is the result of combined activity of Cef and other ferments. Shown is the efficiency of HDS-agar application to determine these characteristics.https://journal.microbe.ru/jour/article/view/870холерный вибрионтвиназаcho cell elongating factorvibrio choleraetweenase |
| spellingShingle | A. B. Mazrukho E. V. Monakhova O. V. Duvanova B. N. Mishan’Kin V. D. Kruglikov Analysis of Genetic Determination of <I>Vibrio cholerae</I> Tweenase Activity Проблемы особо опасных инфекций холерный вибрион твиназа cho cell elongating factor vibrio cholerae tweenase |
| title | Analysis of Genetic Determination of <I>Vibrio cholerae</I> Tweenase Activity |
| title_full | Analysis of Genetic Determination of <I>Vibrio cholerae</I> Tweenase Activity |
| title_fullStr | Analysis of Genetic Determination of <I>Vibrio cholerae</I> Tweenase Activity |
| title_full_unstemmed | Analysis of Genetic Determination of <I>Vibrio cholerae</I> Tweenase Activity |
| title_short | Analysis of Genetic Determination of <I>Vibrio cholerae</I> Tweenase Activity |
| title_sort | analysis of genetic determination of i vibrio cholerae i tweenase activity |
| topic | холерный вибрион твиназа cho cell elongating factor vibrio cholerae tweenase |
| url | https://journal.microbe.ru/jour/article/view/870 |
| work_keys_str_mv | AT abmazrukho analysisofgeneticdeterminationofivibriocholeraeitweenaseactivity AT evmonakhova analysisofgeneticdeterminationofivibriocholeraeitweenaseactivity AT ovduvanova analysisofgeneticdeterminationofivibriocholeraeitweenaseactivity AT bnmishankin analysisofgeneticdeterminationofivibriocholeraeitweenaseactivity AT vdkruglikov analysisofgeneticdeterminationofivibriocholeraeitweenaseactivity |