Taxonomic and Functional Differences between Microbial Communities in Qinghai Lake and Its Input Streams
Understanding microbial communities in terms of taxon and function is essential to decipher the biogeochemical cycling in aquatic ecosystems. Lakes and their input streams are highly linked. However, the differences between microbial assemblages in streams and lakes are still unclear. In this study,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02319/full |
| _version_ | 1818672559231598592 |
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| author | Ze Ren Fang Wang Fang Wang Xiaodong Qu Xiaodong Qu James J. Elser Yang Liu Yang Liu Limin Chu Limin Chu |
| author_facet | Ze Ren Fang Wang Fang Wang Xiaodong Qu Xiaodong Qu James J. Elser Yang Liu Yang Liu Limin Chu Limin Chu |
| author_sort | Ze Ren |
| collection | DOAJ |
| description | Understanding microbial communities in terms of taxon and function is essential to decipher the biogeochemical cycling in aquatic ecosystems. Lakes and their input streams are highly linked. However, the differences between microbial assemblages in streams and lakes are still unclear. In this study, we conducted an intensive field sampling of microbial communities from lake water and stream biofilms in the Qinghai Lake watershed, the largest lake in China. We determined bacterial communities using high-throughput 16S rRNA gene sequencing and predicted functional profiles using PICRUSt to determine the taxonomic and functional differences between microbial communities in stream biofilms and lake water. The results showed that stream biofilms and lake water harbored distinct microbial communities. The microbial communities were different taxonomically and functionally between stream and lake. Moreover, streams biofilms had a microbial network with higher connectivity and modularity than lake water. Functional beta diversity was strongly correlated with taxonomic beta diversity in both the stream and lake microbial communities. Lake microbial assemblages displayed greater predicted metabolic potentials of many metabolism pathways while the microbial assemblages in stream biofilms were more abundant in xenobiotic biodegradation and metabolism and lipid metabolism. Furthermore, lake microbial assemblages had stronger predicted metabolic potentials in amino acid metabolism, carbon fixation, and photosynthesis while stream microbial assemblages were higher in carbohydrate metabolism, oxidative phosphorylation, and nitrogen metabolism. This study adds to our knowledge of stream-lake linkages from the functional and taxonomic composition of microbial assemblages. |
| first_indexed | 2024-12-17T07:41:49Z |
| format | Article |
| id | doaj.art-f5b2e2f2fe5e415cac72ba52db69ce80 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T07:41:49Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f5b2e2f2fe5e415cac72ba52db69ce802022-12-21T21:58:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-11-01810.3389/fmicb.2017.02319303150Taxonomic and Functional Differences between Microbial Communities in Qinghai Lake and Its Input StreamsZe Ren0Fang Wang1Fang Wang2Xiaodong Qu3Xiaodong Qu4James J. Elser5Yang Liu6Yang Liu7Limin Chu8Limin Chu9Flathead Lake Biological Station, University of Montana, Polson, MT, United StatesState Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing, ChinaDepartment of Water Resources, China Institute of Water Resources and Hydropower Research, Beijing, ChinaState Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing, ChinaDepartment of Water Environment, China Institute of Water Resources and Hydropower Research, Beijing, ChinaFlathead Lake Biological Station, University of Montana, Polson, MT, United StatesState Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing, ChinaDepartment of Water Resources, China Institute of Water Resources and Hydropower Research, Beijing, ChinaState Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing, ChinaDepartment of Water Resources, China Institute of Water Resources and Hydropower Research, Beijing, ChinaUnderstanding microbial communities in terms of taxon and function is essential to decipher the biogeochemical cycling in aquatic ecosystems. Lakes and their input streams are highly linked. However, the differences between microbial assemblages in streams and lakes are still unclear. In this study, we conducted an intensive field sampling of microbial communities from lake water and stream biofilms in the Qinghai Lake watershed, the largest lake in China. We determined bacterial communities using high-throughput 16S rRNA gene sequencing and predicted functional profiles using PICRUSt to determine the taxonomic and functional differences between microbial communities in stream biofilms and lake water. The results showed that stream biofilms and lake water harbored distinct microbial communities. The microbial communities were different taxonomically and functionally between stream and lake. Moreover, streams biofilms had a microbial network with higher connectivity and modularity than lake water. Functional beta diversity was strongly correlated with taxonomic beta diversity in both the stream and lake microbial communities. Lake microbial assemblages displayed greater predicted metabolic potentials of many metabolism pathways while the microbial assemblages in stream biofilms were more abundant in xenobiotic biodegradation and metabolism and lipid metabolism. Furthermore, lake microbial assemblages had stronger predicted metabolic potentials in amino acid metabolism, carbon fixation, and photosynthesis while stream microbial assemblages were higher in carbohydrate metabolism, oxidative phosphorylation, and nitrogen metabolism. This study adds to our knowledge of stream-lake linkages from the functional and taxonomic composition of microbial assemblages.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02319/fullco-occurrencefunctionalmicrobial communitystream-lake linkagetaxonomic |
| spellingShingle | Ze Ren Fang Wang Fang Wang Xiaodong Qu Xiaodong Qu James J. Elser Yang Liu Yang Liu Limin Chu Limin Chu Taxonomic and Functional Differences between Microbial Communities in Qinghai Lake and Its Input Streams Frontiers in Microbiology co-occurrence functional microbial community stream-lake linkage taxonomic |
| title | Taxonomic and Functional Differences between Microbial Communities in Qinghai Lake and Its Input Streams |
| title_full | Taxonomic and Functional Differences between Microbial Communities in Qinghai Lake and Its Input Streams |
| title_fullStr | Taxonomic and Functional Differences between Microbial Communities in Qinghai Lake and Its Input Streams |
| title_full_unstemmed | Taxonomic and Functional Differences between Microbial Communities in Qinghai Lake and Its Input Streams |
| title_short | Taxonomic and Functional Differences between Microbial Communities in Qinghai Lake and Its Input Streams |
| title_sort | taxonomic and functional differences between microbial communities in qinghai lake and its input streams |
| topic | co-occurrence functional microbial community stream-lake linkage taxonomic |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02319/full |
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