The Effect of Statins on Blood Gene Expression in COPD.
BACKGROUND:COPD is currently the fourth leading cause of death worldwide. Statins are lipid lowering agents with documented cardiovascular benefits. Observational studies have shown that statins may have a beneficial role in COPD. The impact of statins on blood gene expression from COPD patients is...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4604084?pdf=render |
| _version_ | 1819274037231091712 |
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| author | Ma'en Obeidat Nick Fishbane Yunlong Nie Virginia Chen Zsuzsanna Hollander Scott J Tebbutt Yohan Bossé Raymond T Ng Bruce E Miller Bruce McManus Stephen Rennard Peter D Paré Don D Sin |
| author_facet | Ma'en Obeidat Nick Fishbane Yunlong Nie Virginia Chen Zsuzsanna Hollander Scott J Tebbutt Yohan Bossé Raymond T Ng Bruce E Miller Bruce McManus Stephen Rennard Peter D Paré Don D Sin |
| author_sort | Ma'en Obeidat |
| collection | DOAJ |
| description | BACKGROUND:COPD is currently the fourth leading cause of death worldwide. Statins are lipid lowering agents with documented cardiovascular benefits. Observational studies have shown that statins may have a beneficial role in COPD. The impact of statins on blood gene expression from COPD patients is largely unknown. OBJECTIVE:Identify blood gene signature associated with statin use in COPD patients, and the pathways underpinning this signature that could explain any potential benefits in COPD. METHODS:Whole blood gene expression was measured on 168 statin users and 451 non-users from the ECLIPSE study using the Affymetrix Human Gene 1.1 ST microarray chips. Factor Analysis for Robust Microarray Summarization (FARMS) was used to process the expression data. Differential gene expression analysis was undertaken using the Linear Models for Microarray data (Limma) package adjusting for propensity score and surrogate variables. Similarity of the expression signal with published gene expression profiles was performed in ProfileChaser. RESULTS:25 genes were differentially expressed between statin users and non-users at an FDR of 10%, including LDLR, CXCR2, SC4MOL, FAM108A1, IFI35, FRYL, ABCG1, MYLIP, and DHCR24. The 25 genes were significantly enriched in cholesterol homeostasis and metabolism pathways. The resulting gene signature showed correlation with Huntington's disease, Parkinson's disease and acute myeloid leukemia gene signatures. CONCLUSION:The blood gene signature of statins' use in COPD patients was enriched in cholesterol homeostasis pathways. Further studies are needed to delineate the role of these pathways in lung biology. |
| first_indexed | 2024-12-23T23:02:03Z |
| format | Article |
| id | doaj.art-f5b3a715e81f49a5b3de9c708de72ad8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T23:02:03Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f5b3a715e81f49a5b3de9c708de72ad82022-12-21T17:26:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011010e014002210.1371/journal.pone.0140022The Effect of Statins on Blood Gene Expression in COPD.Ma'en ObeidatNick FishbaneYunlong NieVirginia ChenZsuzsanna HollanderScott J TebbuttYohan BosséRaymond T NgBruce E MillerBruce McManusStephen RennardPeter D ParéDon D SinBACKGROUND:COPD is currently the fourth leading cause of death worldwide. Statins are lipid lowering agents with documented cardiovascular benefits. Observational studies have shown that statins may have a beneficial role in COPD. The impact of statins on blood gene expression from COPD patients is largely unknown. OBJECTIVE:Identify blood gene signature associated with statin use in COPD patients, and the pathways underpinning this signature that could explain any potential benefits in COPD. METHODS:Whole blood gene expression was measured on 168 statin users and 451 non-users from the ECLIPSE study using the Affymetrix Human Gene 1.1 ST microarray chips. Factor Analysis for Robust Microarray Summarization (FARMS) was used to process the expression data. Differential gene expression analysis was undertaken using the Linear Models for Microarray data (Limma) package adjusting for propensity score and surrogate variables. Similarity of the expression signal with published gene expression profiles was performed in ProfileChaser. RESULTS:25 genes were differentially expressed between statin users and non-users at an FDR of 10%, including LDLR, CXCR2, SC4MOL, FAM108A1, IFI35, FRYL, ABCG1, MYLIP, and DHCR24. The 25 genes were significantly enriched in cholesterol homeostasis and metabolism pathways. The resulting gene signature showed correlation with Huntington's disease, Parkinson's disease and acute myeloid leukemia gene signatures. CONCLUSION:The blood gene signature of statins' use in COPD patients was enriched in cholesterol homeostasis pathways. Further studies are needed to delineate the role of these pathways in lung biology.http://europepmc.org/articles/PMC4604084?pdf=render |
| spellingShingle | Ma'en Obeidat Nick Fishbane Yunlong Nie Virginia Chen Zsuzsanna Hollander Scott J Tebbutt Yohan Bossé Raymond T Ng Bruce E Miller Bruce McManus Stephen Rennard Peter D Paré Don D Sin The Effect of Statins on Blood Gene Expression in COPD. PLoS ONE |
| title | The Effect of Statins on Blood Gene Expression in COPD. |
| title_full | The Effect of Statins on Blood Gene Expression in COPD. |
| title_fullStr | The Effect of Statins on Blood Gene Expression in COPD. |
| title_full_unstemmed | The Effect of Statins on Blood Gene Expression in COPD. |
| title_short | The Effect of Statins on Blood Gene Expression in COPD. |
| title_sort | effect of statins on blood gene expression in copd |
| url | http://europepmc.org/articles/PMC4604084?pdf=render |
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