Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against <i>Aedes albopictus</i>
<i>Aedes albopictus</i> is the sole vector for various mosquito-borne viruses, including dengue, chikungunya, and Zika. Ecofriendly biological agents are required to reduce the spread of these mosquito-borne infections. Mosquito densoviruses (MDVs) are entomopathogenic mosquito-specific...
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MDPI AG
2022-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/3/475 |
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| author | Khadija Batool Jie Xiao Ye Xu Ting Yang Peiwen Tao Siyu Zhao Jiao Chen Intikhab Alam Yugu Xie Jinbao Gu Xiaoguang Chen |
| author_facet | Khadija Batool Jie Xiao Ye Xu Ting Yang Peiwen Tao Siyu Zhao Jiao Chen Intikhab Alam Yugu Xie Jinbao Gu Xiaoguang Chen |
| author_sort | Khadija Batool |
| collection | DOAJ |
| description | <i>Aedes albopictus</i> is the sole vector for various mosquito-borne viruses, including dengue, chikungunya, and Zika. Ecofriendly biological agents are required to reduce the spread of these mosquito-borne infections. Mosquito densoviruses (MDVs) are entomopathogenic mosquito-specific viruses, which can reduce the capacity of isolated vectors and decrease mosquito-borne viral disease transmission. However, their variable pathogenicity restricts their commercial use. In the present study, we developed a series of novel larvicide oil suspensions (denoted <i>Bacillus thuringiensis</i> (<i>Bti</i>) oil, <i>Ae. albopictus</i> densovirus (AalDV-5) oil, and a mixture of AalDV-5+<i>Bti</i> oil), which were tested against <i>Ae. albopictus</i> larvae under experimental semi-field and open-field conditions. The effect of AalDV-5 on non-target species was also evaluated. The combined effect of AalDV-5+<i>Bti</i> was greater than that of individual toxins and was longer lasting and more persistent compared with the laboratory AalDV-5 virus strain. The virus was quantified on a weekly basis by quantitative polymerase chain reaction (qPCR) and was persistently detected in rearing water as well as in dead larvae. Wildtype densovirus is not pathogenic to non-target organisms. The present findings confirm the improved effect of a mixed microbial suspension (AalDV-5+<i>Bti</i> oil) larvicide against <i>Ae. albopictus</i>. The development and testing of these products will enable better control of the vector mosquitoes. |
| first_indexed | 2024-03-09T12:17:21Z |
| format | Article |
| id | doaj.art-f5b8b5a3239a4a769c4cd982d8b9a213 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:17:21Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f5b8b5a3239a4a769c4cd982d8b9a2132023-11-30T22:45:07ZengMDPI AGViruses1999-49152022-02-0114347510.3390/v14030475Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against <i>Aedes albopictus</i>Khadija Batool0Jie Xiao1Ye Xu2Ting Yang3Peiwen Tao4Siyu Zhao5Jiao Chen6Intikhab Alam7Yugu Xie8Jinbao Gu9Xiaoguang Chen10Department of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaWuhan Baile Health Technology Co., Ltd., Wuhan 430100, ChinaWuhan Baile Health Technology Co., Ltd., Wuhan 430100, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaWuhan Baile Health Technology Co., Ltd., Wuhan 430100, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China<i>Aedes albopictus</i> is the sole vector for various mosquito-borne viruses, including dengue, chikungunya, and Zika. Ecofriendly biological agents are required to reduce the spread of these mosquito-borne infections. Mosquito densoviruses (MDVs) are entomopathogenic mosquito-specific viruses, which can reduce the capacity of isolated vectors and decrease mosquito-borne viral disease transmission. However, their variable pathogenicity restricts their commercial use. In the present study, we developed a series of novel larvicide oil suspensions (denoted <i>Bacillus thuringiensis</i> (<i>Bti</i>) oil, <i>Ae. albopictus</i> densovirus (AalDV-5) oil, and a mixture of AalDV-5+<i>Bti</i> oil), which were tested against <i>Ae. albopictus</i> larvae under experimental semi-field and open-field conditions. The effect of AalDV-5 on non-target species was also evaluated. The combined effect of AalDV-5+<i>Bti</i> was greater than that of individual toxins and was longer lasting and more persistent compared with the laboratory AalDV-5 virus strain. The virus was quantified on a weekly basis by quantitative polymerase chain reaction (qPCR) and was persistently detected in rearing water as well as in dead larvae. Wildtype densovirus is not pathogenic to non-target organisms. The present findings confirm the improved effect of a mixed microbial suspension (AalDV-5+<i>Bti</i> oil) larvicide against <i>Ae. albopictus</i>. The development and testing of these products will enable better control of the vector mosquitoes.https://www.mdpi.com/1999-4915/14/3/475<i>Aedes albopictus</i>densovirus<i>Bacillus thuringiensis</i>mosquito vectorlarvicidebiological control |
| spellingShingle | Khadija Batool Jie Xiao Ye Xu Ting Yang Peiwen Tao Siyu Zhao Jiao Chen Intikhab Alam Yugu Xie Jinbao Gu Xiaoguang Chen Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against <i>Aedes albopictus</i> Viruses <i>Aedes albopictus</i> densovirus <i>Bacillus thuringiensis</i> mosquito vector larvicide biological control |
| title | Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against <i>Aedes albopictus</i> |
| title_full | Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against <i>Aedes albopictus</i> |
| title_fullStr | Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against <i>Aedes albopictus</i> |
| title_full_unstemmed | Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against <i>Aedes albopictus</i> |
| title_short | Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against <i>Aedes albopictus</i> |
| title_sort | densovirus oil suspension significantly improves the efficacy and duration of larvicidal activity against i aedes albopictus i |
| topic | <i>Aedes albopictus</i> densovirus <i>Bacillus thuringiensis</i> mosquito vector larvicide biological control |
| url | https://www.mdpi.com/1999-4915/14/3/475 |
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