Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk
Bovine mastitis is an important disease of cattle which affects economy of the country. The purpose of study was isolation and identification of the common bacterial causes of clinical bovine mastitis. To this end, 50 bovine milk samples from CMT confirmed-clinical mastitis cases were collected and...
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| Format: | Article |
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Journal of Pure and Applied Microbiology
2017-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/isolation-and-identification-of-common-mastitis-causing-pathogens-from-clinical-bovine-mastitic-milk/ |
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| author | Anjali Gangwal S.K. Kashyap Sushil Katiyar Dheerendra Meena Pramod Boyal Kapil Gupta |
| author_facet | Anjali Gangwal S.K. Kashyap Sushil Katiyar Dheerendra Meena Pramod Boyal Kapil Gupta |
| author_sort | Anjali Gangwal |
| collection | DOAJ |
| description | Bovine mastitis is an important disease of cattle which affects economy of the country. The purpose of study was isolation and identification of the common bacterial causes of clinical bovine mastitis. To this end, 50 bovine milk samples from CMT confirmed-clinical mastitis cases were collected and cultured on Nutrient agar, Blood agar and Mac-Conkey agar plates for bacterial isolation. The major pathogens isolated were Escherichia coli (28%), Staphylococcus aureus (24%), Pseudomonas aeruginosa (18%), Klebsiella pneumoniae (10%) and Streptococcus sp. (2%) as per the biochemical tests and PCR. In conclusion, high percentage of positive samples and relatively high occurrence of environmental microorganisms indicates urgent need to emphisize udder hygiene for clean milk production. |
| first_indexed | 2024-12-19T00:13:39Z |
| format | Article |
| id | doaj.art-f5bc2790f5a3480882ea1c51f3faa08f |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-19T00:13:39Z |
| publishDate | 2017-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-f5bc2790f5a3480882ea1c51f3faa08f2022-12-21T20:45:56ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-03-0111132933310.22207/JPAM.11.1.41Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic MilkAnjali Gangwal0S.K. Kashyap1Sushil Katiyar2Dheerendra Meena3Pramod Boyal4Kapil Gupta5Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner -334 001, India.Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner -334 001, India.Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner -334 001, India.Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner -334 001, India.Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner -334 001, India.Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner -334 001, India.Bovine mastitis is an important disease of cattle which affects economy of the country. The purpose of study was isolation and identification of the common bacterial causes of clinical bovine mastitis. To this end, 50 bovine milk samples from CMT confirmed-clinical mastitis cases were collected and cultured on Nutrient agar, Blood agar and Mac-Conkey agar plates for bacterial isolation. The major pathogens isolated were Escherichia coli (28%), Staphylococcus aureus (24%), Pseudomonas aeruginosa (18%), Klebsiella pneumoniae (10%) and Streptococcus sp. (2%) as per the biochemical tests and PCR. In conclusion, high percentage of positive samples and relatively high occurrence of environmental microorganisms indicates urgent need to emphisize udder hygiene for clean milk production.https://microbiologyjournal.org/isolation-and-identification-of-common-mastitis-causing-pathogens-from-clinical-bovine-mastitic-milk/bovine mastitispcrstaphylococcuse. coli |
| spellingShingle | Anjali Gangwal S.K. Kashyap Sushil Katiyar Dheerendra Meena Pramod Boyal Kapil Gupta Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk Journal of Pure and Applied Microbiology bovine mastitis pcr staphylococcus e. coli |
| title | Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk |
| title_full | Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk |
| title_fullStr | Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk |
| title_full_unstemmed | Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk |
| title_short | Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk |
| title_sort | isolation and identification of common mastitis causing pathogens from clinical bovine mastitic milk |
| topic | bovine mastitis pcr staphylococcus e. coli |
| url | https://microbiologyjournal.org/isolation-and-identification-of-common-mastitis-causing-pathogens-from-clinical-bovine-mastitic-milk/ |
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