Diferenciação de micobactérias por PCR multiplex Differentiation of micobacteria by multiplex PCR

Diferenciação de micobactérias por PCR multiplex Differentiation of micobacteria by multiplex PCR

O trabalho visou à otimização de um método baseado na reação em cadeia da polimerase multiplex - para diferenciação de micobactérias de interesse para a saúde pública. A PCR Multiplex baseou-se na amplificação simultânea do genehsp65, presente em todo gênero Mycobacterium, do gene dnaJ, presente ape...

Full description

Bibliographic Details
Main Authors:	Diogo da Rocha Poroca, Andrea Santos Lima, Juliana Falcão de Araújo Lima, Heidi Lacerda Alves da Cruz, Rosana de Albuquerque Montenegro, Fábio Lopes de Melo, Haiana Charifker Schindler, Lílian Maria Lapa Montenegro
Format:	Article
Language:	English
Published:	Sociedade Brasileira de Medicina Tropical (SBMT) 2009-12-01
Series:	Revista da Sociedade Brasileira de Medicina Tropical
Subjects:	Micobactérias PCR multiplex IS6110 dnaJ hsp65 Mycobacteria Multiplex PCR
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822009000600020

Similar Items

Global Screening and Functional Identification of Major HSPs Involved in PVY Infection in Potato
by: Kun Li, et al.
Published: (2022-03-01)

Establishing and validating the molecular identification of mycobacterial infection on direct patient samples
by: K. M. A. Karunathilaka, et al.
Published: (2021-12-01)

The J‐ and G/F‐domains of the major Synechocystis DnaJ protein Sll0897 are sufficient for cell viability but not for heat resistance
by: Eva Düppre, et al.
Published: (2020-11-01)

Ab initio protein structure prediction: the necessary presence of external force field as it is delivered by Hsp40 chaperone
by: Irena Roterman, et al.
Published: (2023-11-01)

Identificación de micobacterias atípicas aisladas de muestras clínicas en el Laboratorio Central de Salud Pública, Paraguay (2010-2013)
by: Rosmary Franco, et al.
Published: (2023-05-01)

DnaK and DnaJ proteins from Hsp70/40 family are involved in Rubisco biosynthesis in Synechocystis sp. PCC6803 and sustain the enzyme assembly in a heterologous system
by: Małgorzata Rydzy, et al.
Published: (2023-02-01)

Editorial: Pathologic Conditions of the Human Nervous and Muscular Systems Associated with Mutant Chaperones: Molecular and Mechanistic Aspects
by: Alberto J. L. Macario, et al.
Published: (2018-02-01)

DnaJ Proteins Regulate <i>WUS</i> Expression in Shoot Apical Meristem of Arabidopsis
by: Tianqi Jia, et al.
Published: (2021-01-01)

Evaluating the Sensitivity of Different Molecular Techniques for Detecting Mycobacterium tuberculosis Complex in Patients with Pulmonary Infection
by: Hemeg Hassan A., et al.
Published: (2023-12-01)

COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO
by: Fathiah Zakham, et al.
Published: (2012-01-01)

HSP70/DNAJ Family of Genes in the Brown Planthopper, <i>Nilaparvata lugens</i>: Diversity and Function
by: Xuan Chen, et al.
Published: (2021-03-01)

A METHOD FOR DETECTING AND TYPING OF SALMONELLA BY MULTIPLEX PCR
by: Borys Stegniy, et al.
Published: (2015-01-01)

A single, multiplex PCR for differentiating all species of Trichinella
by: Zarlenga D.S., et al.
Published: (2001-06-01)

Serum-isolated exosomes from Piscirickettsia salmonis-infected Salmo salar specimens enclose bacterial DnaK, DnaJ and GrpE chaperones
by: Cristián Muñoz, et al.
Published: (2022-09-01)

Bacterial Hsp90 Facilitates the Degradation of Aggregation-Prone Hsp70–Hsp40 Substrates
by: Bruno Fauvet, et al.
Published: (2021-04-01)

Global analysis of expression profile of members of DnaJ gene families involved in capsaicinoids synthesis in pepper (Capsicum annuum L)
by: Fang Fei Fan, et al.
Published: (2020-07-01)

DnaJ homolog subfamily A member1 (DnaJ1) is a newly discovered anti-apoptotic protein regulated by azadirachtin in Sf9 cells
by: Benshui Shu, et al.
Published: (2018-05-01)

Rapid detection of Mycobacterium tuberculosis complex: PCR method using insertion sequence 6110
by: Rohani M, et al.
Published: (2009-06-01)

Constructing a DNA ladder Range for Lambda Phage by multiplex PCR
by: R Gopalakrishnan, et al.
Published: (2010-12-01)

In-house PCR with DNA extracted directly from positive slides to confirm or exclude the diagnosis of tuberculosis: focus on biosafety
by: Isabela Neves de Almeida, et al.
Published: (2015-03-01)

Molecular Characterization of Environmental Non-Tuberculous Mycobacteria Using PCR- RFLP Analysis of 441 Bp Heat Shock Protein 65 Fragments
by: H Rezaei-Yazdi, et al.
Published: (2012-04-01)

Molecular Characterization of Environmental Non-Tuberculous Mycobacteria Using PCR- RFLP Analysis of 441 Bp Heat Shock Protein 65 Fragments
by: B Nasr-Esfahani, et al.
Published: (2012-04-01)

MsDjB4, a HSP40 Chaperone in Alfalfa (<i>Medicago sativa</i> L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress
by: Siyan Liu, et al.
Published: (2023-07-01)

Genome-Wide Identification and Characterization of <i>DnaJ</i> Gene Family in Grape (<i>Vitis vinifera</i> L.)
by: Tianchi Chen, et al.
Published: (2021-12-01)

Genome-wide analysis of soybean DnaJA-family genes and functional characterization of GmDnaJA6 responses to saline and alkaline stress
by: Binshuo Zhang, et al.
Published: (2023-08-01)

Genome-wide identification of DnaJ gene family in Catalpa bungei and functional analysis of CbuDnaJ49 in leaf color formation
by: Yingying Yang, et al.
Published: (2023-02-01)

A novel gene therapy for methamphetamine- induced cognitive disorder with a hyper-acidified fusion variant of DnaJB1
by: Mengru Zhang, et al.
Published: (2023-03-01)

Genes for Yield Stability in Tomatoes
by: Josef Fisher, et al.
Published: (2021-12-01)

Avaliação do protocolo PCR4 de Marchetti em tecidos parafinizados para o diagnóstico da tuberculose cutânea e ganglionar Evaluation of Marchetti PCR4 amplification assay to the diagnosis of cutaneous and lymph node tuberculosis from formalin-fixed paraffin-embedded tissue
by: Luciana Botinelly Mendonça Fujimoto, et al.
Published: (2007-06-01)

Comparative diagnostic value of GeneXpert MTB/RIF, PCR targeting IS6110 and culture for the diagnosis of spinal tuberculosis
by: Yoyos Ismiarto Ismiarto, et al.
Published: (2021-12-01)

ZnJ2 Is a Member of a Large Chaperone Family in the Chloroplast of Photosynthetic Organisms that Features a DnaJ-Like Zn-Finger Domain
by: Lior Doron, et al.
Published: (2018-02-01)

Diagnóstico molecular da tuberculose pulmonar Molecular diagnosis of pulmonary tuberculosis
by: Nelma Cristina Sousa de Assis, et al.
Published: (2007-02-01)

A multiplex PCR assay for the simultaneous detection of Taenia hydatigena, T. multiceps, T. pisiformis, and Dipylidium caninum infections
by: Guo-Qiang Zhu, et al.
Published: (2019-10-01)

The Nuclear Localization of the DnaJ-Like Zinc Finger Domain-Containing Protein EDA3 Affects Seed Development in <i>Arabidopsis thaliana</i>
by: Meng-Juan Kong, et al.
Published: (2020-10-01)

Ectopic expression of DnaJ type-I protein homolog of Vigna aconitifolia (VaDJI) confers ABA insensitivity and multiple stress tolerance in transgenic tobacco plants
by: Ranjana Gautam, et al.
Published: (2023-04-01)

Molecular detection of Mycobacterium tuberculosis complex in bovine blood samples
by: N. Sarika, et al.
Published: (2022-12-01)

Role of multiplex polymerase chain reaction using IS6110 and Protein b for the diagnosis of extra-pulmonary tuberculosis: North India
by: Kusum Sharma, et al.
Published: (2015-01-01)

Comparison of LCD array and IS6110-PCR with conventional techniques for detection of Mycobacterium bovis isolated from Egyptian cattle and Buffaloes
by: Rasha Nabil Zahran, et al.
Published: (2014-01-01)

Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
by: Mehrdad Ravanshad, et al.
Published: (2009-12-01)

Development of a multiplex pcr method for direct detection of common mastitis pathogens in bovine milk samples
by: Recep KALIN, et al.
Published: (2017-07-01)