Multifunctional biocatalysis: An unusual imine reductase
A multifunctional biocatalyst EneIRED capable of catalyzing amine-activated conjugate alkene reduction and subsequent reductive amination was discovered. The enzyme realized the coupling of α, β-unsaturated carbonyls with amines to efficiently synthesize a broad set of chiral amine diastereomers bas...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Engineering Microbiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667370322000145 |
| _version_ | 1797788428342919168 |
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| author | Feifei Chen Jianhe Xu Gaowei Zheng |
| author_facet | Feifei Chen Jianhe Xu Gaowei Zheng |
| author_sort | Feifei Chen |
| collection | DOAJ |
| description | A multifunctional biocatalyst EneIRED capable of catalyzing amine-activated conjugate alkene reduction and subsequent reductive amination was discovered. The enzyme realized the coupling of α, β-unsaturated carbonyls with amines to efficiently synthesize a broad set of chiral amine diastereomers based on its unusual active site structure and catalytic mechanism. |
| first_indexed | 2024-03-13T01:36:25Z |
| format | Article |
| id | doaj.art-f5bfb20c9d874489b96e86508c910f88 |
| institution | Directory Open Access Journal |
| issn | 2667-3703 |
| language | English |
| last_indexed | 2024-03-13T01:36:25Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Engineering Microbiology |
| spelling | doaj.art-f5bfb20c9d874489b96e86508c910f882023-07-04T05:11:23ZengElsevierEngineering Microbiology2667-37032022-06-0122100023Multifunctional biocatalysis: An unusual imine reductaseFeifei Chen0Jianhe Xu1Gaowei Zheng2State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology,Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology,Shanghai 200237, ChinaCorresponding author.; State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology,Shanghai 200237, ChinaA multifunctional biocatalyst EneIRED capable of catalyzing amine-activated conjugate alkene reduction and subsequent reductive amination was discovered. The enzyme realized the coupling of α, β-unsaturated carbonyls with amines to efficiently synthesize a broad set of chiral amine diastereomers based on its unusual active site structure and catalytic mechanism.http://www.sciencedirect.com/science/article/pii/S2667370322000145Imine reductaseBiocatalysisChiral amineConjugate reductionReductive amination |
| spellingShingle | Feifei Chen Jianhe Xu Gaowei Zheng Multifunctional biocatalysis: An unusual imine reductase Engineering Microbiology Imine reductase Biocatalysis Chiral amine Conjugate reduction Reductive amination |
| title | Multifunctional biocatalysis: An unusual imine reductase |
| title_full | Multifunctional biocatalysis: An unusual imine reductase |
| title_fullStr | Multifunctional biocatalysis: An unusual imine reductase |
| title_full_unstemmed | Multifunctional biocatalysis: An unusual imine reductase |
| title_short | Multifunctional biocatalysis: An unusual imine reductase |
| title_sort | multifunctional biocatalysis an unusual imine reductase |
| topic | Imine reductase Biocatalysis Chiral amine Conjugate reduction Reductive amination |
| url | http://www.sciencedirect.com/science/article/pii/S2667370322000145 |
| work_keys_str_mv | AT feifeichen multifunctionalbiocatalysisanunusualiminereductase AT jianhexu multifunctionalbiocatalysisanunusualiminereductase AT gaoweizheng multifunctionalbiocatalysisanunusualiminereductase |