Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles
Abstract Background Silver nanoparticles (AgNPs) are used extensively in various consumer products because of their antimicrobial potential. This requires insight in their potential hazards and risks including adverse effects during pregnancy on the developing fetus. Using a combination of the BeWo...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Particle and Fibre Toxicology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12989-020-00342-6 |
| _version_ | 1818320668520873984 |
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| author | Ashraf Abdelkhaliq Meike van der Zande Ruud J. B. Peters Hans Bouwmeester |
| author_facet | Ashraf Abdelkhaliq Meike van der Zande Ruud J. B. Peters Hans Bouwmeester |
| author_sort | Ashraf Abdelkhaliq |
| collection | DOAJ |
| description | Abstract Background Silver nanoparticles (AgNPs) are used extensively in various consumer products because of their antimicrobial potential. This requires insight in their potential hazards and risks including adverse effects during pregnancy on the developing fetus. Using a combination of the BeWo b30 placental transport model and the mouse embryonic stem cell test (EST), we investigated the capability of pristine AgNPs with different surface chemistries and aged AgNPs (silver sulfide (Ag2S) NPs) to cross the placental barrier and induce developmental toxicity. The uptake/association and transport of AgNPs through the BeWo b30 was characterized using ICP-MS and single particle (sp)ICP-MS at different time points. The developmental toxicity of the AgNPs was investigated by characterizing their potential to inhibit the differentiation of mouse embryonic stem cells (mESCs) into beating cardiomyocytes. Results The AgNPs are able to cross the BeWo b30 cell layer to a level that was limited and dependent on their surface chemistry. In the EST, no in vitro developmental toxicity was observed as the effects on differentiation of the mESCs were only detected at cytotoxic concentrations. The aged AgNPs were significantly less cytotoxic, less bioavailable and did not induce developmental toxicity. Conclusions Pristine AgNPs are capable to cross the placental barrier to an extent that is influenced by their surface chemistry and that this transport is likely low but not negligible. Next to that, the tested AgNPs have low intrinsic potencies for developmental toxicity. The combination of the BeWo b30 model with the EST is of added value in developmental toxicity screening and prioritization of AgNPs. |
| first_indexed | 2024-12-13T10:28:40Z |
| format | Article |
| id | doaj.art-f5c1b8b280fd4b2291ae6bdfce55a90a |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-12-13T10:28:40Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-f5c1b8b280fd4b2291ae6bdfce55a90a2022-12-21T23:50:56ZengBMCParticle and Fibre Toxicology1743-89772020-03-0117111610.1186/s12989-020-00342-6Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticlesAshraf Abdelkhaliq0Meike van der Zande1Ruud J. B. Peters2Hans Bouwmeester3Division of Toxicology, Wageningen UniversityWageningen Food Safety Research (WFSR)Wageningen Food Safety Research (WFSR)Division of Toxicology, Wageningen UniversityAbstract Background Silver nanoparticles (AgNPs) are used extensively in various consumer products because of their antimicrobial potential. This requires insight in their potential hazards and risks including adverse effects during pregnancy on the developing fetus. Using a combination of the BeWo b30 placental transport model and the mouse embryonic stem cell test (EST), we investigated the capability of pristine AgNPs with different surface chemistries and aged AgNPs (silver sulfide (Ag2S) NPs) to cross the placental barrier and induce developmental toxicity. The uptake/association and transport of AgNPs through the BeWo b30 was characterized using ICP-MS and single particle (sp)ICP-MS at different time points. The developmental toxicity of the AgNPs was investigated by characterizing their potential to inhibit the differentiation of mouse embryonic stem cells (mESCs) into beating cardiomyocytes. Results The AgNPs are able to cross the BeWo b30 cell layer to a level that was limited and dependent on their surface chemistry. In the EST, no in vitro developmental toxicity was observed as the effects on differentiation of the mESCs were only detected at cytotoxic concentrations. The aged AgNPs were significantly less cytotoxic, less bioavailable and did not induce developmental toxicity. Conclusions Pristine AgNPs are capable to cross the placental barrier to an extent that is influenced by their surface chemistry and that this transport is likely low but not negligible. Next to that, the tested AgNPs have low intrinsic potencies for developmental toxicity. The combination of the BeWo b30 model with the EST is of added value in developmental toxicity screening and prioritization of AgNPs.http://link.springer.com/article/10.1186/s12989-020-00342-6Silver nanoparticlesSurface chemistryPlacental transportEmbryotoxicitySingle particle-ICP-MS |
| spellingShingle | Ashraf Abdelkhaliq Meike van der Zande Ruud J. B. Peters Hans Bouwmeester Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles Particle and Fibre Toxicology Silver nanoparticles Surface chemistry Placental transport Embryotoxicity Single particle-ICP-MS |
| title | Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles |
| title_full | Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles |
| title_fullStr | Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles |
| title_full_unstemmed | Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles |
| title_short | Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles |
| title_sort | combination of the bewo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles |
| topic | Silver nanoparticles Surface chemistry Placental transport Embryotoxicity Single particle-ICP-MS |
| url | http://link.springer.com/article/10.1186/s12989-020-00342-6 |
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