Epigallocatechin gallate inhibits hepatitis B virus infection in human liver chimeric mice
Abstract Background Persistent hepatitis B virus (HBV) infection causes liver cirrhosis and hepatocellular carcinoma and constitutes a major worldwide health problem. Currently, anti-HBV drugs are limited to peginterferon and nucleos(t)ide analogs, which are costly and have considerable side effects...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2316-4 |
| _version_ | 1811222090600153088 |
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| author | Yu-Heng Lai Cheng-Pu Sun Hsiu-Chen Huang Jui-Chieh Chen Hui-Kang Liu Cheng Huang |
| author_facet | Yu-Heng Lai Cheng-Pu Sun Hsiu-Chen Huang Jui-Chieh Chen Hui-Kang Liu Cheng Huang |
| author_sort | Yu-Heng Lai |
| collection | DOAJ |
| description | Abstract Background Persistent hepatitis B virus (HBV) infection causes liver cirrhosis and hepatocellular carcinoma and constitutes a major worldwide health problem. Currently, anti-HBV drugs are limited to peginterferon and nucleos(t)ide analogs, which are costly and have considerable side effects; the development of novel, effective anti-HBV agents is crucial. Methods Catechins are a major group of compounds found in green tea extract and epigallocatechin gallate (EGCG) has been shown to have antiviral properties, including inhibition of cellular entry by HBV. FRG (Fah−/−/ Rag2−/−/ IL-2Rγ/−) mice were used in this study to generate chimeras carrying human primary hepatocytes, to facilitate investigation of the inhibitory effect of EGCG on HBV infection. Results Here, we show the inhibitory effect of EGCG on HBV infection and replication in HuS-E/2 cells. The inhibitory effect of EGCG on HBV infection in vivo was confirmed by monitoring HBV DNA and HBsAg in serum and immunostaining the liver tissues of the human liver chimeric mice. Conclusions The effects of EGCG suggest a robust strategy for the treatment of HBV infection and EGCG may have therapeutic potential for the treatment of HBV-associated liver diseases. |
| first_indexed | 2024-04-12T08:10:42Z |
| format | Article |
| id | doaj.art-f5c5985b85be4820aa3441930d118d41 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-04-12T08:10:42Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-f5c5985b85be4820aa3441930d118d412022-12-22T03:40:59ZengBMCBMC Complementary and Alternative Medicine1472-68822018-09-011811710.1186/s12906-018-2316-4Epigallocatechin gallate inhibits hepatitis B virus infection in human liver chimeric miceYu-Heng Lai0Cheng-Pu Sun1Hsiu-Chen Huang2Jui-Chieh Chen3Hui-Kang Liu4Cheng Huang5Department of Chemistry, Chinese Culture UniversityInstitute of Biomedical Sciences, Academia SinicaDepartment of Applied Science, National Tsing Hua University South CampusDepartment of Biochemical Science and Technology, National Chiayi UniversityNational Research Institute of Chinese Medicine, Ministry of Health and WelfareDepartment of Biotechnology and Laboratory Science in Medicine, National Yang-Ming UniversityAbstract Background Persistent hepatitis B virus (HBV) infection causes liver cirrhosis and hepatocellular carcinoma and constitutes a major worldwide health problem. Currently, anti-HBV drugs are limited to peginterferon and nucleos(t)ide analogs, which are costly and have considerable side effects; the development of novel, effective anti-HBV agents is crucial. Methods Catechins are a major group of compounds found in green tea extract and epigallocatechin gallate (EGCG) has been shown to have antiviral properties, including inhibition of cellular entry by HBV. FRG (Fah−/−/ Rag2−/−/ IL-2Rγ/−) mice were used in this study to generate chimeras carrying human primary hepatocytes, to facilitate investigation of the inhibitory effect of EGCG on HBV infection. Results Here, we show the inhibitory effect of EGCG on HBV infection and replication in HuS-E/2 cells. The inhibitory effect of EGCG on HBV infection in vivo was confirmed by monitoring HBV DNA and HBsAg in serum and immunostaining the liver tissues of the human liver chimeric mice. Conclusions The effects of EGCG suggest a robust strategy for the treatment of HBV infection and EGCG may have therapeutic potential for the treatment of HBV-associated liver diseases.http://link.springer.com/article/10.1186/s12906-018-2316-4Hepatitis B virusEGCGHBsAgHuman liver chimeric mice |
| spellingShingle | Yu-Heng Lai Cheng-Pu Sun Hsiu-Chen Huang Jui-Chieh Chen Hui-Kang Liu Cheng Huang Epigallocatechin gallate inhibits hepatitis B virus infection in human liver chimeric mice BMC Complementary and Alternative Medicine Hepatitis B virus EGCG HBsAg Human liver chimeric mice |
| title | Epigallocatechin gallate inhibits hepatitis B virus infection in human liver chimeric mice |
| title_full | Epigallocatechin gallate inhibits hepatitis B virus infection in human liver chimeric mice |
| title_fullStr | Epigallocatechin gallate inhibits hepatitis B virus infection in human liver chimeric mice |
| title_full_unstemmed | Epigallocatechin gallate inhibits hepatitis B virus infection in human liver chimeric mice |
| title_short | Epigallocatechin gallate inhibits hepatitis B virus infection in human liver chimeric mice |
| title_sort | epigallocatechin gallate inhibits hepatitis b virus infection in human liver chimeric mice |
| topic | Hepatitis B virus EGCG HBsAg Human liver chimeric mice |
| url | http://link.springer.com/article/10.1186/s12906-018-2316-4 |
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