Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum
The study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular analyses of ITS and TEF genes and host-specificity test on cucurbits including me...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2014-12-01
|
| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/journal/view.html?uid=2&sort=book_Seq&scale=&key=year&keyword=&s_v=20&s_n=4&pn=vol&year=2014&vmd=Full |
| _version_ | 1819242442299277312 |
|---|---|
| author | Ji Hyun Lee Yong Ho Choi Kyoung Soo Jang Jin-Cheol Kim Gyung Ja Choi |
| author_facet | Ji Hyun Lee Yong Ho Choi Kyoung Soo Jang Jin-Cheol Kim Gyung Ja Choi |
| author_sort | Ji Hyun Lee |
| collection | DOAJ |
| description | The study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum
f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular
analyses of ITS and TEF genes and host-specificity test on cucurbits including melon, oriental melon,
cucumber, and watermelon. Then four cucumber and two rootstock cultivars showing different resistance
degrees to the Fusarium wilt pathogen KR5 were selected. And development of Fusarium wilt of the six cultivars
according to several conditions, including incubation temperature after inoculation, inoculum concentration,
root wounding, and growth stages of seedlings, was investigated. Disease severity of Fusarium wilt
on the resistant cultivars was changed with incubation temperatures after inoculation. The resistant cultivars
showed the higher resistance when inoculated plants were kept at 25 or 30oC than at 20oC. Among four different
growth stages of the seedlings, seven-day-old seedling represented the most difference of resistance and
susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant
cucumber to F. oxysporum f. sp. cucumerinum is to dip the non-cut roots of seven-day-old seedlings in spore
suspension of 1.0 × 106-1.0 × 107 conidia/ml and to transplant the seedling into a non-infected soil, and then
to incubate the inoculated plants in a growth room at 25oC for 3 weeks to develop Fusarium wilt. |
| first_indexed | 2024-12-23T14:39:52Z |
| format | Article |
| id | doaj.art-f5c62ebeff2e4118b826c71c3af9b64b |
| institution | Directory Open Access Journal |
| issn | 1598-2262 2233-9191 |
| language | English |
| last_indexed | 2024-12-23T14:39:52Z |
| publishDate | 2014-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-f5c62ebeff2e4118b826c71c3af9b64b2022-12-21T17:43:14ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912014-12-01204245252http://dx.doi.org/10.5423/RPD.2014.20.4.245Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinumJi Hyun Lee0Yong Ho Choi1Kyoung Soo Jang2Jin-Cheol Kim3Gyung Ja Choi4Korea Research Institute of Chemical Technology, koreaKorea Research Institute of Chemical Technology, koreaKorea Research Institute of Chemical Technology, koreaChonnam National University, Gwangju 500-757, KoreaKorea Research Institute of Chemical Technology, koreaThe study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular analyses of ITS and TEF genes and host-specificity test on cucurbits including melon, oriental melon, cucumber, and watermelon. Then four cucumber and two rootstock cultivars showing different resistance degrees to the Fusarium wilt pathogen KR5 were selected. And development of Fusarium wilt of the six cultivars according to several conditions, including incubation temperature after inoculation, inoculum concentration, root wounding, and growth stages of seedlings, was investigated. Disease severity of Fusarium wilt on the resistant cultivars was changed with incubation temperatures after inoculation. The resistant cultivars showed the higher resistance when inoculated plants were kept at 25 or 30oC than at 20oC. Among four different growth stages of the seedlings, seven-day-old seedling represented the most difference of resistance and susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant cucumber to F. oxysporum f. sp. cucumerinum is to dip the non-cut roots of seven-day-old seedlings in spore suspension of 1.0 × 106-1.0 × 107 conidia/ml and to transplant the seedling into a non-infected soil, and then to incubate the inoculated plants in a growth room at 25oC for 3 weeks to develop Fusarium wilt.http://www.online-rpd.org/journal/view.html?uid=2&sort=book_Seq&scale=&key=year&keyword=&s_v=20&s_n=4&pn=vol&year=2014&vmd=FullBreedingDisease resistanceFusarium wilt |
| spellingShingle | Ji Hyun Lee Yong Ho Choi Kyoung Soo Jang Jin-Cheol Kim Gyung Ja Choi Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum Research in Plant Disease Breeding Disease resistance Fusarium wilt |
| title | Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum |
| title_full | Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum |
| title_fullStr | Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum |
| title_full_unstemmed | Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum |
| title_short | Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum |
| title_sort | efficient screening method for resistance of cucumber cultivars to fusarium oxysporum f sp cucumerinum |
| topic | Breeding Disease resistance Fusarium wilt |
| url | http://www.online-rpd.org/journal/view.html?uid=2&sort=book_Seq&scale=&key=year&keyword=&s_v=20&s_n=4&pn=vol&year=2014&vmd=Full |
| work_keys_str_mv | AT jihyunlee efficientscreeningmethodforresistanceofcucumbercultivarstofusariumoxysporumfspcucumerinum AT yonghochoi efficientscreeningmethodforresistanceofcucumbercultivarstofusariumoxysporumfspcucumerinum AT kyoungsoojang efficientscreeningmethodforresistanceofcucumbercultivarstofusariumoxysporumfspcucumerinum AT jincheolkim efficientscreeningmethodforresistanceofcucumbercultivarstofusariumoxysporumfspcucumerinum AT gyungjachoi efficientscreeningmethodforresistanceofcucumbercultivarstofusariumoxysporumfspcucumerinum |