Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
Phenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain re...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-09-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867011702318 |
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| author | Carlos Henrique Camargo Ariane Bruder-Nascimento, MSc; PhD Alessandro Lia Mondelli, PhD Augusto Cezar Montelli Terue Sadatsune, PhD |
| author_facet | Carlos Henrique Camargo Ariane Bruder-Nascimento, MSc; PhD Alessandro Lia Mondelli, PhD Augusto Cezar Montelli Terue Sadatsune, PhD |
| author_sort | Carlos Henrique Camargo |
| collection | DOAJ |
| description | Phenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either blaSPM or blaIMP genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of blaSPM and blaIMP genes among Brazilian clinical isolates. Keywords: Pseudomonas aeruginosa, drug resistance, bacterial, carbapenems, metalloproteins, polymerase chain reaction |
| first_indexed | 2024-12-11T16:17:44Z |
| format | Article |
| id | doaj.art-f5c67aec83a34da198e82cff1d361c07 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-11T16:17:44Z |
| publishDate | 2011-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-f5c67aec83a34da198e82cff1d361c072022-12-22T00:58:55ZengElsevierBrazilian Journal of Infectious Diseases1413-86702011-09-01155478481Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospitalCarlos Henrique Camargo0Ariane Bruder-Nascimento, MSc; PhD1Alessandro Lia Mondelli, PhD2Augusto Cezar Montelli3Terue Sadatsune, PhD4Biologist; MSc Student, Universidade Estatual Paulista (UNESP), Botucatu, SP, Brazil; Correspondence to: Universidade Estadual Paulista Instituto de Biociências de Botucatu Departamento de Microbiologia e Imunologia Distrito de Rubião Jr., s/n, Rubião Jr., 18618-000, Botucatu, SP, Brazil.Student, UNESP, Botucatu, SP, BrazilProfessors, UNESP, Botucatu, SP, BrazilPost-doc; Professor, UNESP, Botucatu, SP, BrazilProfessors, UNESP, Botucatu, SP, BrazilPhenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either blaSPM or blaIMP genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of blaSPM and blaIMP genes among Brazilian clinical isolates. Keywords: Pseudomonas aeruginosa, drug resistance, bacterial, carbapenems, metalloproteins, polymerase chain reactionhttp://www.sciencedirect.com/science/article/pii/S1413867011702318 |
| spellingShingle | Carlos Henrique Camargo Ariane Bruder-Nascimento, MSc; PhD Alessandro Lia Mondelli, PhD Augusto Cezar Montelli Terue Sadatsune, PhD Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital Brazilian Journal of Infectious Diseases |
| title | Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital |
| title_full | Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital |
| title_fullStr | Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital |
| title_full_unstemmed | Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital |
| title_short | Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital |
| title_sort | detection of spm and imp metallo β lactamases in clinical specimens of pseudomonas aeruginosa from a brazilian public tertiary hospital |
| url | http://www.sciencedirect.com/science/article/pii/S1413867011702318 |
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