3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment
Physical signals provided by the extracellular matrix (ECM) are key microenvironmental parameters for the fate decision of hematopoietic stem and progenitor cells (HSPC) in bone marrow. Insights into cell-ECM interactions are critical for advancing HSC-based tissue engineering. Herein, we employed c...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2022-04-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21004084 |
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| author | Pan Zhang Linmu Xu Jingsong Gao Guangkui Xu Yanping Song Guang Li Jingjing Ren Yunjie Zhang Cheng Yang Yu Zhang Ruiheng Xie Nu Zhang Hui Yang |
| author_facet | Pan Zhang Linmu Xu Jingsong Gao Guangkui Xu Yanping Song Guang Li Jingjing Ren Yunjie Zhang Cheng Yang Yu Zhang Ruiheng Xie Nu Zhang Hui Yang |
| author_sort | Pan Zhang |
| collection | DOAJ |
| description | Physical signals provided by the extracellular matrix (ECM) are key microenvironmental parameters for the fate decision of hematopoietic stem and progenitor cells (HSPC) in bone marrow. Insights into cell-ECM interactions are critical for advancing HSC-based tissue engineering. Herein, we employed collagen hydrogels and collagen-alginate hydrogels of defined stiffness to study the behaviors of hematopoietic progenitor cells (HPCs). Three-dimensional (3D) collagen hydrogels with a stiffness of 45 Pa were found to promote HPC maintenance and colony formation of monocyte/macrophage progenitors. Using single-cell RNA sequencing (scRNA-seq), we also characterized the comprehensive transcriptional profiles of cells randomly selected from two-dimensional (2D) and 3D hydrogels. A distinct maturation trajectory from HPCs into macrophages within the 3D microenvironment was revealed by these results. 3D-derived macrophages expressed high levels of various cytokines and chemokines, such as Saa3, Cxcl2, Socs3 and Tnf. Furthermore, enhanced communication between 3D-macrophages and other hematopoietic clusters based on ligand-repair interactions was demonstrated through bioinformatic analyses. Our research underlines the regulatory role of matrix-dimensionality in HPC differentiation and therefore probably be applied to the generation of specialized macrophages. |
| first_indexed | 2024-04-24T08:30:46Z |
| format | Article |
| id | doaj.art-f5c8d6db82a34a58bf2f58abfab572c3 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:30:46Z |
| publishDate | 2022-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-f5c8d6db82a34a58bf2f58abfab572c32024-04-16T20:20:36ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2022-04-01102552683D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitmentPan Zhang0Linmu Xu1Jingsong Gao2Guangkui Xu3Yanping Song4Guang Li5Jingjing Ren6Yunjie Zhang7Cheng Yang8Yu Zhang9Ruiheng Xie10Nu Zhang11Hui Yang12School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China; Research Center of Special Environmental Biomechanics & Medical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China; Research Center of Special Environmental Biomechanics & Medical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China; Research Center of Special Environmental Biomechanics & Medical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, ChinaSchool of Aerospace Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, ChinaInstitute of Hematology, Xi'an Central Hospital, Xi'an, Shaanxi, 710003, ChinaInstitute of Hematology, Xi'an Central Hospital, Xi'an, Shaanxi, 710003, ChinaInstitute of Hematology, Xi'an Central Hospital, Xi'an, Shaanxi, 710003, ChinaInstitute of Hematology, Xi'an Central Hospital, Xi'an, Shaanxi, 710003, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China; Research Center of Special Environmental Biomechanics & Medical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, ChinaSchool of Computer Science, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, ChinaDepartment of Computer and Information Science, University of Delaware, Newark, DE, USASchool of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China; Research Center of Special Environmental Biomechanics & Medical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China; Research Center of Special Environmental Biomechanics & Medical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China; Corresponding author. Northwestern Polytechnical University, #127 West Youyi Road, Xi'an, Shaanxi 710072, China.Physical signals provided by the extracellular matrix (ECM) are key microenvironmental parameters for the fate decision of hematopoietic stem and progenitor cells (HSPC) in bone marrow. Insights into cell-ECM interactions are critical for advancing HSC-based tissue engineering. Herein, we employed collagen hydrogels and collagen-alginate hydrogels of defined stiffness to study the behaviors of hematopoietic progenitor cells (HPCs). Three-dimensional (3D) collagen hydrogels with a stiffness of 45 Pa were found to promote HPC maintenance and colony formation of monocyte/macrophage progenitors. Using single-cell RNA sequencing (scRNA-seq), we also characterized the comprehensive transcriptional profiles of cells randomly selected from two-dimensional (2D) and 3D hydrogels. A distinct maturation trajectory from HPCs into macrophages within the 3D microenvironment was revealed by these results. 3D-derived macrophages expressed high levels of various cytokines and chemokines, such as Saa3, Cxcl2, Socs3 and Tnf. Furthermore, enhanced communication between 3D-macrophages and other hematopoietic clusters based on ligand-repair interactions was demonstrated through bioinformatic analyses. Our research underlines the regulatory role of matrix-dimensionality in HPC differentiation and therefore probably be applied to the generation of specialized macrophages.http://www.sciencedirect.com/science/article/pii/S2452199X21004084Hematopoietic progenitor cellsCollagenMatrix-dimensionalityMacrophages |
| spellingShingle | Pan Zhang Linmu Xu Jingsong Gao Guangkui Xu Yanping Song Guang Li Jingjing Ren Yunjie Zhang Cheng Yang Yu Zhang Ruiheng Xie Nu Zhang Hui Yang 3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment Bioactive Materials Hematopoietic progenitor cells Collagen Matrix-dimensionality Macrophages |
| title | 3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment |
| title_full | 3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment |
| title_fullStr | 3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment |
| title_full_unstemmed | 3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment |
| title_short | 3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment |
| title_sort | 3d collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment |
| topic | Hematopoietic progenitor cells Collagen Matrix-dimensionality Macrophages |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21004084 |
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