| Summary: | The goal of the present research was to screen the antimicrobial activity of an ethanolic extract of <i>Kitaibelia vitifolia</i> against 30 multidrug-resistant (MDR) bacterial strains isolated from healthcare-associated infections. Minimum inhibitory concentrations (MICs) of the samples against the tested bacteria were determined using the microdilution method. MDR bacterial strains were characterized using standard biochemical tests and the commercial identification systems API 20 NE and API 20 E as: <i>Klebsiella</i> spp. (18 isolates—I); methicillin-resistant <i>Staphylococcus aureus</i> (MRSA)—3; <i>Acinetobacter</i> spp.—3; <i>Pseudomonas aeruginosa</i>—5; vancomycin-resistant <i>Enterococcus</i> (VRE)—1. The sensitivity of isolated bacterial strains was determined using the disc diffusion method against 25 commonly used antibiotics. The highest level of sensitivity to <i>K. vitifolia</i> extract was confirmed in 88.89% of <i>Klebsiella</i> spp. isolates, <i>E. coli</i> ATCC 25922, two strains of MRSA (1726, 1063), <i>Acinetobacter</i> spp. strain 1578, and VRE strain 30, like <i>Enterococcus faecalis</i> ATCC 29212 (MIC =< 2.44 μg/mL). The lowest sensitivity was exhibited by 75.00% of <i>Acinetobacter</i> spp. (strains 1577 and 6401), where the highest values for MICs were noted (1250 μg/mL). The results indicate that the extract of <i>K. vitifolia</i> could be a possible source for creating new, efficient, and effective natural medicines for combat against MDR strains of bacteria.
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