Evaluation of Antimicrobial Activity of <i>Kitaibelia vitifolia</i> Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical Origin
The goal of the present research was to screen the antimicrobial activity of an ethanolic extract of <i>Kitaibelia vitifolia</i> against 30 multidrug-resistant (MDR) bacterial strains isolated from healthcare-associated infections. Minimum inhibitory concentrations (MICs) of the samples...
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MDPI AG
2023-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/18/3236 |
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| author | Vladimir S. Kurćubić Svetlana V. Raketić Jelena M. Mašković Pavle Z. Mašković Luka V. Kurćubić Volker Heinz Igor B. Tomasevic |
| author_facet | Vladimir S. Kurćubić Svetlana V. Raketić Jelena M. Mašković Pavle Z. Mašković Luka V. Kurćubić Volker Heinz Igor B. Tomasevic |
| author_sort | Vladimir S. Kurćubić |
| collection | DOAJ |
| description | The goal of the present research was to screen the antimicrobial activity of an ethanolic extract of <i>Kitaibelia vitifolia</i> against 30 multidrug-resistant (MDR) bacterial strains isolated from healthcare-associated infections. Minimum inhibitory concentrations (MICs) of the samples against the tested bacteria were determined using the microdilution method. MDR bacterial strains were characterized using standard biochemical tests and the commercial identification systems API 20 NE and API 20 E as: <i>Klebsiella</i> spp. (18 isolates—I); methicillin-resistant <i>Staphylococcus aureus</i> (MRSA)—3; <i>Acinetobacter</i> spp.—3; <i>Pseudomonas aeruginosa</i>—5; vancomycin-resistant <i>Enterococcus</i> (VRE)—1. The sensitivity of isolated bacterial strains was determined using the disc diffusion method against 25 commonly used antibiotics. The highest level of sensitivity to <i>K. vitifolia</i> extract was confirmed in 88.89% of <i>Klebsiella</i> spp. isolates, <i>E. coli</i> ATCC 25922, two strains of MRSA (1726, 1063), <i>Acinetobacter</i> spp. strain 1578, and VRE strain 30, like <i>Enterococcus faecalis</i> ATCC 29212 (MIC =< 2.44 μg/mL). The lowest sensitivity was exhibited by 75.00% of <i>Acinetobacter</i> spp. (strains 1577 and 6401), where the highest values for MICs were noted (1250 μg/mL). The results indicate that the extract of <i>K. vitifolia</i> could be a possible source for creating new, efficient, and effective natural medicines for combat against MDR strains of bacteria. |
| first_indexed | 2024-03-10T22:13:54Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T22:13:54Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-f5cb639e8e984c7fa5f5ed27117ddec92023-11-19T12:32:00ZengMDPI AGPlants2223-77472023-09-011218323610.3390/plants12183236Evaluation of Antimicrobial Activity of <i>Kitaibelia vitifolia</i> Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical OriginVladimir S. Kurćubić0Svetlana V. Raketić1Jelena M. Mašković2Pavle Z. Mašković3Luka V. Kurćubić4Volker Heinz5Igor B. Tomasevic6Department of Food Technology, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32000 Čačak, SerbiaMicrobiology Laboratory for Food and Water, Public Health Institute Čačak, Veselina Milikića 7, 32000 Čačak, SerbiaDepartment of Chemistry and Chemical Engineering, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32000 Čačak, SerbiaDepartment of Chemistry and Chemical Engineering, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32000 Čačak, SerbiaDepartment of Medical Microbiology, University Clinical Center of Serbia, Pasterova 2, 11000 Beograd, SerbiaDIL German Institute of Food Technology, Prof.-von-Klitzing-Str. 7, D-49610 Quakenbrück, GermanyDIL German Institute of Food Technology, Prof.-von-Klitzing-Str. 7, D-49610 Quakenbrück, GermanyThe goal of the present research was to screen the antimicrobial activity of an ethanolic extract of <i>Kitaibelia vitifolia</i> against 30 multidrug-resistant (MDR) bacterial strains isolated from healthcare-associated infections. Minimum inhibitory concentrations (MICs) of the samples against the tested bacteria were determined using the microdilution method. MDR bacterial strains were characterized using standard biochemical tests and the commercial identification systems API 20 NE and API 20 E as: <i>Klebsiella</i> spp. (18 isolates—I); methicillin-resistant <i>Staphylococcus aureus</i> (MRSA)—3; <i>Acinetobacter</i> spp.—3; <i>Pseudomonas aeruginosa</i>—5; vancomycin-resistant <i>Enterococcus</i> (VRE)—1. The sensitivity of isolated bacterial strains was determined using the disc diffusion method against 25 commonly used antibiotics. The highest level of sensitivity to <i>K. vitifolia</i> extract was confirmed in 88.89% of <i>Klebsiella</i> spp. isolates, <i>E. coli</i> ATCC 25922, two strains of MRSA (1726, 1063), <i>Acinetobacter</i> spp. strain 1578, and VRE strain 30, like <i>Enterococcus faecalis</i> ATCC 29212 (MIC =< 2.44 μg/mL). The lowest sensitivity was exhibited by 75.00% of <i>Acinetobacter</i> spp. (strains 1577 and 6401), where the highest values for MICs were noted (1250 μg/mL). The results indicate that the extract of <i>K. vitifolia</i> could be a possible source for creating new, efficient, and effective natural medicines for combat against MDR strains of bacteria.https://www.mdpi.com/2223-7747/12/18/3236<i>Kitaibelia vitifolia</i>multidrug resistant bacteriaantibacterial activitydisc diffusion methodminimum inhibitory concentration |
| spellingShingle | Vladimir S. Kurćubić Svetlana V. Raketić Jelena M. Mašković Pavle Z. Mašković Luka V. Kurćubić Volker Heinz Igor B. Tomasevic Evaluation of Antimicrobial Activity of <i>Kitaibelia vitifolia</i> Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical Origin Plants <i>Kitaibelia vitifolia</i> multidrug resistant bacteria antibacterial activity disc diffusion method minimum inhibitory concentration |
| title | Evaluation of Antimicrobial Activity of <i>Kitaibelia vitifolia</i> Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical Origin |
| title_full | Evaluation of Antimicrobial Activity of <i>Kitaibelia vitifolia</i> Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical Origin |
| title_fullStr | Evaluation of Antimicrobial Activity of <i>Kitaibelia vitifolia</i> Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical Origin |
| title_full_unstemmed | Evaluation of Antimicrobial Activity of <i>Kitaibelia vitifolia</i> Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical Origin |
| title_short | Evaluation of Antimicrobial Activity of <i>Kitaibelia vitifolia</i> Extract against Proven Antibiotic-Susceptible and Multidrug-Resistant (MDR) Strains of Bacteria of Clinical Origin |
| title_sort | evaluation of antimicrobial activity of i kitaibelia vitifolia i extract against proven antibiotic susceptible and multidrug resistant mdr strains of bacteria of clinical origin |
| topic | <i>Kitaibelia vitifolia</i> multidrug resistant bacteria antibacterial activity disc diffusion method minimum inhibitory concentration |
| url | https://www.mdpi.com/2223-7747/12/18/3236 |
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