Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in Mice
Lack of sleep time is a menace to modern people, and it leads to chronic diseases and mental illnesses. Circadian processes control sleep, but little is known about how sleep affects the circadian system. Therefore, we performed a 28-day sleep restriction (SR) treatment in mice. Sleep restriction di...
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MDPI AG
2022-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/2/42 |
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| author | Weitian Li Zixu Wang Jing Cao Yulan Dong Yaoxing Chen |
| author_facet | Weitian Li Zixu Wang Jing Cao Yulan Dong Yaoxing Chen |
| author_sort | Weitian Li |
| collection | DOAJ |
| description | Lack of sleep time is a menace to modern people, and it leads to chronic diseases and mental illnesses. Circadian processes control sleep, but little is known about how sleep affects the circadian system. Therefore, we performed a 28-day sleep restriction (SR) treatment in mice. Sleep restriction disrupted the clock genes’ circadian rhythm. The circadian rhythms of the <i>Cry1</i> and <i>Per1/2/3</i> genes disappeared. The acrophase of the clock genes (<i>Bmal1, Clock, Rev-erbα</i>, and <i>Rorβ</i>) that still had a circadian rhythm was advanced, while the acrophase of negative clock gene <i>Cry2</i> was delayed. Clock genes’ upstream signals ERK and EIFs also had circadian rhythm disorders. Accompanied by changes in the central oscillator, the plasma output signal (melatonin, corticosterone, IL-6, and TNF-α) had an advanced acrophase. While the melatonin mesor was decreased, the corticosterone, IL-6, and TNF-α mesor was increased. Our results indicated that chronic sleep loss could disrupt the circadian rhythm of the central clock through ERK and EIFs and affect the output signal downstream of the core biological clock. |
| first_indexed | 2024-03-09T22:17:47Z |
| format | Article |
| id | doaj.art-f5cb79c463cd42a98a79c3d3edbe2f5c |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T22:17:47Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-f5cb79c463cd42a98a79c3d3edbe2f5c2023-11-23T19:20:56ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-01-0144260962510.3390/cimb44020042Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in MiceWeitian Li0Zixu Wang1Jing Cao2Yulan Dong3Yaoxing Chen4Neurobiology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaNeurobiology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaNeurobiology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaNeurobiology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaNeurobiology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaLack of sleep time is a menace to modern people, and it leads to chronic diseases and mental illnesses. Circadian processes control sleep, but little is known about how sleep affects the circadian system. Therefore, we performed a 28-day sleep restriction (SR) treatment in mice. Sleep restriction disrupted the clock genes’ circadian rhythm. The circadian rhythms of the <i>Cry1</i> and <i>Per1/2/3</i> genes disappeared. The acrophase of the clock genes (<i>Bmal1, Clock, Rev-erbα</i>, and <i>Rorβ</i>) that still had a circadian rhythm was advanced, while the acrophase of negative clock gene <i>Cry2</i> was delayed. Clock genes’ upstream signals ERK and EIFs also had circadian rhythm disorders. Accompanied by changes in the central oscillator, the plasma output signal (melatonin, corticosterone, IL-6, and TNF-α) had an advanced acrophase. While the melatonin mesor was decreased, the corticosterone, IL-6, and TNF-α mesor was increased. Our results indicated that chronic sleep loss could disrupt the circadian rhythm of the central clock through ERK and EIFs and affect the output signal downstream of the core biological clock.https://www.mdpi.com/1467-3045/44/2/42circadian rhythmclock genesleep restrictionhypothalamic |
| spellingShingle | Weitian Li Zixu Wang Jing Cao Yulan Dong Yaoxing Chen Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in Mice Current Issues in Molecular Biology circadian rhythm clock gene sleep restriction hypothalamic |
| title | Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in Mice |
| title_full | Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in Mice |
| title_fullStr | Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in Mice |
| title_full_unstemmed | Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in Mice |
| title_short | Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in Mice |
| title_sort | role of sleep restriction in daily rhythms of expression of hypothalamic core clock genes in mice |
| topic | circadian rhythm clock gene sleep restriction hypothalamic |
| url | https://www.mdpi.com/1467-3045/44/2/42 |
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