Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso
The estimates of enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> burden in developing countries are limited by the lack of rapid, accessible, and sensitive diagnostics and surveillance tools. We used a “Rapid LAMP based Diagnostic Test (RLDT)” to detect ETE...
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| Formato: | Artigo |
| Idioma: | English |
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MDPI AG
2023-11-01
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| Colecção: | Microorganisms |
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| Acesso em linha: | https://www.mdpi.com/2076-2607/11/11/2809 |
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| author | Alimatou Héma Samuel S. Sermé Jean Sawadogo Amidou Diarra Aissata Barry Amidou Z. Ouédraogo Issa Nébié Alfred B. Tiono Sophie Houard Subhra Chakraborty Alphonse Ouédraogo Sodiomon B. Sirima |
| author_facet | Alimatou Héma Samuel S. Sermé Jean Sawadogo Amidou Diarra Aissata Barry Amidou Z. Ouédraogo Issa Nébié Alfred B. Tiono Sophie Houard Subhra Chakraborty Alphonse Ouédraogo Sodiomon B. Sirima |
| author_sort | Alimatou Héma |
| collection | DOAJ |
| description | The estimates of enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> burden in developing countries are limited by the lack of rapid, accessible, and sensitive diagnostics and surveillance tools. We used a “Rapid LAMP based Diagnostic Test (RLDT)” to detect ETEC and <i>Shigella</i> in diarrheal and non-diarrheal stool samples from a 12-month longitudinal cohort of children under five years of age in a peri-urban area of Ouagadougou in Burkina Faso (West Africa). To allow comparison with the RLDT-<i>Shigella</i> results, conventional culture methods were used to identify <i>Shigella</i> strains in the stool samples. As conventional culture alone cannot detect ETEC cases, a subset of <i>E. coli</i>-like colonies was tested using conventional PCR to detect ETEC toxins genes. Of the 165 stool samples analyzed for ETEC, 24.9% were positive when using RLDT against 4.2% when using culture followed by PCR. ETEC toxin distribution when using RLDT was STp 17.6% (29/165), LT 11.5% (19/165), and STh 8.5% (14/165). Of the 263 specimens tested for <i>Shigella</i>, 44.8% were positive when using RLDT against 23.2% when using culture. The sensitivity and specificity of the RLDT compared to culture (followed by PCR for ETEC) were 93.44% and 69.8% for <i>Shigella</i> and 83.7% and 77.9% for ETEC, respectively. This study indicates that both <i>Shigella</i> and ETEC are substantially underdiagnosed when using conventional culture and highlights the potential contribution of the new RLDT method to improve enteric disease burden estimation and to guide future efforts to prevent and control bacterial enteric infection and disease. |
| first_indexed | 2024-03-09T16:35:48Z |
| format | Article |
| id | doaj.art-f5cec5cc839149bf8851eea3f489b7e1 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:35:48Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f5cec5cc839149bf8851eea3f489b7e12023-11-24T14:57:20ZengMDPI AGMicroorganisms2076-26072023-11-011111280910.3390/microorganisms11112809Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina FasoAlimatou Héma0Samuel S. Sermé1Jean Sawadogo2Amidou Diarra3Aissata Barry4Amidou Z. Ouédraogo5Issa Nébié6Alfred B. Tiono7Sophie Houard8Subhra Chakraborty9Alphonse Ouédraogo10Sodiomon B. Sirima11Groupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoEuropean Vaccine Initiative (EVI), Universitäts Klinikum Heidelberg Vossstrasse 2, Geb. 4040, 69115 Heidelberg, GermanyDepartment of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USAGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoGroupe de Recherche Action en Santé (GRAS), Ouagadougou 06 BP 10248, Burkina FasoThe estimates of enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> burden in developing countries are limited by the lack of rapid, accessible, and sensitive diagnostics and surveillance tools. We used a “Rapid LAMP based Diagnostic Test (RLDT)” to detect ETEC and <i>Shigella</i> in diarrheal and non-diarrheal stool samples from a 12-month longitudinal cohort of children under five years of age in a peri-urban area of Ouagadougou in Burkina Faso (West Africa). To allow comparison with the RLDT-<i>Shigella</i> results, conventional culture methods were used to identify <i>Shigella</i> strains in the stool samples. As conventional culture alone cannot detect ETEC cases, a subset of <i>E. coli</i>-like colonies was tested using conventional PCR to detect ETEC toxins genes. Of the 165 stool samples analyzed for ETEC, 24.9% were positive when using RLDT against 4.2% when using culture followed by PCR. ETEC toxin distribution when using RLDT was STp 17.6% (29/165), LT 11.5% (19/165), and STh 8.5% (14/165). Of the 263 specimens tested for <i>Shigella</i>, 44.8% were positive when using RLDT against 23.2% when using culture. The sensitivity and specificity of the RLDT compared to culture (followed by PCR for ETEC) were 93.44% and 69.8% for <i>Shigella</i> and 83.7% and 77.9% for ETEC, respectively. This study indicates that both <i>Shigella</i> and ETEC are substantially underdiagnosed when using conventional culture and highlights the potential contribution of the new RLDT method to improve enteric disease burden estimation and to guide future efforts to prevent and control bacterial enteric infection and disease.https://www.mdpi.com/2076-2607/11/11/2809enterotoxigenic <i>Escherichia coli</i><i>Shigella</i>children under fiveRLDTBurkina Faso |
| spellingShingle | Alimatou Héma Samuel S. Sermé Jean Sawadogo Amidou Diarra Aissata Barry Amidou Z. Ouédraogo Issa Nébié Alfred B. Tiono Sophie Houard Subhra Chakraborty Alphonse Ouédraogo Sodiomon B. Sirima Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso Microorganisms enterotoxigenic <i>Escherichia coli</i> <i>Shigella</i> children under five RLDT Burkina Faso |
| title | Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso |
| title_full | Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso |
| title_fullStr | Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso |
| title_full_unstemmed | Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso |
| title_short | Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic <i>Escherichia coli</i> (ETEC) and <i>Shigella</i> in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso |
| title_sort | contribution of the rapid lamp based diagnostic test rldt to the evaluation of enterotoxigenic i escherichia coli i etec and i shigella i in childhood diarrhea in the peri urban area of ouagadougou burkina faso |
| topic | enterotoxigenic <i>Escherichia coli</i> <i>Shigella</i> children under five RLDT Burkina Faso |
| url | https://www.mdpi.com/2076-2607/11/11/2809 |
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