<i>Epsc</i> Involved in the Encoding of Exopolysaccharides Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Act to Boost the Drought Tolerance of <i>Arabidopsis thaliana</i>
<i>Bacillus amyloliquefaciens</i> FZB42 is a plant growth-promoting rhizobacteria that stimulates plant growth, and enhances resistance to pathogens and tolerance of salt stress. Instead, the mechanistic basis of drought tolerance in <i>Arabidopsis</i> <i>thaliana</i...
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MDPI AG
2018-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/19/12/3795 |
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| author | Xiang Lu Shao-Fang Liu Liang Yue Xia Zhao Yu-Bao Zhang Zhong-Kui Xie Ruo-Yu Wang |
| author_facet | Xiang Lu Shao-Fang Liu Liang Yue Xia Zhao Yu-Bao Zhang Zhong-Kui Xie Ruo-Yu Wang |
| author_sort | Xiang Lu |
| collection | DOAJ |
| description | <i>Bacillus amyloliquefaciens</i> FZB42 is a plant growth-promoting rhizobacteria that stimulates plant growth, and enhances resistance to pathogens and tolerance of salt stress. Instead, the mechanistic basis of drought tolerance in <i>Arabidopsis</i> <i>thaliana</i> induced by FZB42 remains unexplored. Here, we constructed an exopolysaccharide-deficient mutant <i>epsC</i> and determined the role of <i>epsC</i> in FZB42-induced drought tolerance in <i>A. thaliana</i>. Results showed that FZB42 significantly enhanced growth and drought tolerance of <i>Arabidopsis</i> by increasing the survival rate, fresh and dry shoot weights, primary root length, root dry weight, lateral root number, and total lateral root length. Coordinated changes were also observed in cellular defense responses, including elevated concentrations of proline and activities of superoxide dismutase and peroxidase, decreased concentrations of malondialdehyde, and accumulation of hydrogen peroxide in plants treated with FZB42. The relative expression levels of drought defense-related marker genes, such as <i>RD29A</i>, <i>RD17</i>, <i>ERD1</i>, and <i>LEA14</i>, were also increased in the leaves of FZB42-treated plants. In addition, FZB42 induced the drought tolerance in <i>Arabidopsis</i> by the action of both ethylene and jasmonate, but not abscisic acid. However, plants inoculated with mutant strain <i>epsC</i> were less able to resist drought stress with respect to each of these parameters, indicating that <i>epsC</i> are required for the full benefit of FZB42 inoculation to be gained. Moreover, the mutant strain was less capable of supporting the formation of a biofilm and of colonizing the <i>A. thaliana</i> root. Therefore, <i>epsC</i> is an important factor that allows FZB42 to colonize the roots and induce systemic drought tolerance in <i>Arabidopsis</i>. |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T19:44:48Z |
| publishDate | 2018-11-01 |
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| spelling | doaj.art-f5d06c66091a4406803587382633a5432022-12-22T03:19:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-11-011912379510.3390/ijms19123795ijms19123795<i>Epsc</i> Involved in the Encoding of Exopolysaccharides Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Act to Boost the Drought Tolerance of <i>Arabidopsis thaliana</i>Xiang Lu0Shao-Fang Liu1Liang Yue2Xia Zhao3Yu-Bao Zhang4Zhong-Kui Xie5Ruo-Yu Wang6Gaolan Station of Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaGaolan Station of Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaGaolan Station of Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaGaolan Station of Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaGaolan Station of Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaGaolan Station of Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaGaolan Station of Agricultural and Ecological Experiment, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China<i>Bacillus amyloliquefaciens</i> FZB42 is a plant growth-promoting rhizobacteria that stimulates plant growth, and enhances resistance to pathogens and tolerance of salt stress. Instead, the mechanistic basis of drought tolerance in <i>Arabidopsis</i> <i>thaliana</i> induced by FZB42 remains unexplored. Here, we constructed an exopolysaccharide-deficient mutant <i>epsC</i> and determined the role of <i>epsC</i> in FZB42-induced drought tolerance in <i>A. thaliana</i>. Results showed that FZB42 significantly enhanced growth and drought tolerance of <i>Arabidopsis</i> by increasing the survival rate, fresh and dry shoot weights, primary root length, root dry weight, lateral root number, and total lateral root length. Coordinated changes were also observed in cellular defense responses, including elevated concentrations of proline and activities of superoxide dismutase and peroxidase, decreased concentrations of malondialdehyde, and accumulation of hydrogen peroxide in plants treated with FZB42. The relative expression levels of drought defense-related marker genes, such as <i>RD29A</i>, <i>RD17</i>, <i>ERD1</i>, and <i>LEA14</i>, were also increased in the leaves of FZB42-treated plants. In addition, FZB42 induced the drought tolerance in <i>Arabidopsis</i> by the action of both ethylene and jasmonate, but not abscisic acid. However, plants inoculated with mutant strain <i>epsC</i> were less able to resist drought stress with respect to each of these parameters, indicating that <i>epsC</i> are required for the full benefit of FZB42 inoculation to be gained. Moreover, the mutant strain was less capable of supporting the formation of a biofilm and of colonizing the <i>A. thaliana</i> root. Therefore, <i>epsC</i> is an important factor that allows FZB42 to colonize the roots and induce systemic drought tolerance in <i>Arabidopsis</i>.https://www.mdpi.com/1422-0067/19/12/3795<i>Bacillus amyloliquefaciens</i> FZB42biofilmdrought stress<i>epsC</i>exopolysaccharidesphytohormone |
| spellingShingle | Xiang Lu Shao-Fang Liu Liang Yue Xia Zhao Yu-Bao Zhang Zhong-Kui Xie Ruo-Yu Wang <i>Epsc</i> Involved in the Encoding of Exopolysaccharides Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Act to Boost the Drought Tolerance of <i>Arabidopsis thaliana</i> International Journal of Molecular Sciences <i>Bacillus amyloliquefaciens</i> FZB42 biofilm drought stress <i>epsC</i> exopolysaccharides phytohormone |
| title | <i>Epsc</i> Involved in the Encoding of Exopolysaccharides Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Act to Boost the Drought Tolerance of <i>Arabidopsis thaliana</i> |
| title_full | <i>Epsc</i> Involved in the Encoding of Exopolysaccharides Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Act to Boost the Drought Tolerance of <i>Arabidopsis thaliana</i> |
| title_fullStr | <i>Epsc</i> Involved in the Encoding of Exopolysaccharides Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Act to Boost the Drought Tolerance of <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | <i>Epsc</i> Involved in the Encoding of Exopolysaccharides Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Act to Boost the Drought Tolerance of <i>Arabidopsis thaliana</i> |
| title_short | <i>Epsc</i> Involved in the Encoding of Exopolysaccharides Produced by <i>Bacillus amyloliquefaciens</i> FZB42 Act to Boost the Drought Tolerance of <i>Arabidopsis thaliana</i> |
| title_sort | i epsc i involved in the encoding of exopolysaccharides produced by i bacillus amyloliquefaciens i fzb42 act to boost the drought tolerance of i arabidopsis thaliana i |
| topic | <i>Bacillus amyloliquefaciens</i> FZB42 biofilm drought stress <i>epsC</i> exopolysaccharides phytohormone |
| url | https://www.mdpi.com/1422-0067/19/12/3795 |
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