Identification of Peanut <i>Aux/IAA</i> Genes and Functional Prediction during Seed Development and Maturation
Auxin-responsive genes <i>AUX/IAA</i> are important during plant growth and development, but there are few relevant reports in peanut. In this study, 44 <i>AhIAA</i> genes were identified from cultivated peanut, of which 31 genes were expressed in seed at varying degrees. <...
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/4/472 |
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| author | Xiurong Zhang Kun Zhang Lu Luo Yuying Lv Yuying Li Suqing Zhu Bing Luo Yongshan Wan Xiansheng Zhang Fengzhen Liu |
| author_facet | Xiurong Zhang Kun Zhang Lu Luo Yuying Lv Yuying Li Suqing Zhu Bing Luo Yongshan Wan Xiansheng Zhang Fengzhen Liu |
| author_sort | Xiurong Zhang |
| collection | DOAJ |
| description | Auxin-responsive genes <i>AUX/IAA</i> are important during plant growth and development, but there are few relevant reports in peanut. In this study, 44 <i>AhIAA</i> genes were identified from cultivated peanut, of which 31 genes were expressed in seed at varying degrees. <i>AhIAA-3A</i>, <i>AhIAA-16A</i> and <i>AhIAA-15B</i> were up-regulated, while <i>AhIAA-11A</i>, <i>AhIAA-5B</i> and <i>AhIAA-14B</i> were down-regulated with seed development and maturation. The expression patterns of seven genes, <i>AhIAA-1A</i>, <i>AhIAA-4A</i>, <i>AhIAA-10A</i>, <i>AhIAA-20A</i>, <i>AhIAA-1B</i>, <i>AhIAA-4B</i> and <i>AhIAA-19B</i>, were consistent with the change trend of auxin, and expression in late-maturing variety LM was significantly higher than that in early-maturing EM. Furthermore, allelic polymorphism analysis of <i>AhIAA-1A</i> and <i>AhIAA-1B</i>, which were specifically expressed in seeds, showed that three SNP loci in 3′UTR of <i>AhIAA-1A</i> could effectively distinguish the EM- and LM- type germplasm, providing a basis for breeding markers development. Our results offered a comprehensive understanding of <i>Aux/IAA</i> genes in peanut and provided valuable clues for further investigation of the auxin signal transduction pathway and auxin regulation mechanism in peanut. |
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| language | English |
| last_indexed | 2024-03-09T21:13:33Z |
| publishDate | 2022-02-01 |
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| series | Plants |
| spelling | doaj.art-f5d2604d2f584d7f9de4aa64cbaed94b2023-11-23T21:41:56ZengMDPI AGPlants2223-77472022-02-0111447210.3390/plants11040472Identification of Peanut <i>Aux/IAA</i> Genes and Functional Prediction during Seed Development and MaturationXiurong Zhang0Kun Zhang1Lu Luo2Yuying Lv3Yuying Li4Suqing Zhu5Bing Luo6Yongshan Wan7Xiansheng Zhang8Fengzhen Liu9State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaAuxin-responsive genes <i>AUX/IAA</i> are important during plant growth and development, but there are few relevant reports in peanut. In this study, 44 <i>AhIAA</i> genes were identified from cultivated peanut, of which 31 genes were expressed in seed at varying degrees. <i>AhIAA-3A</i>, <i>AhIAA-16A</i> and <i>AhIAA-15B</i> were up-regulated, while <i>AhIAA-11A</i>, <i>AhIAA-5B</i> and <i>AhIAA-14B</i> were down-regulated with seed development and maturation. The expression patterns of seven genes, <i>AhIAA-1A</i>, <i>AhIAA-4A</i>, <i>AhIAA-10A</i>, <i>AhIAA-20A</i>, <i>AhIAA-1B</i>, <i>AhIAA-4B</i> and <i>AhIAA-19B</i>, were consistent with the change trend of auxin, and expression in late-maturing variety LM was significantly higher than that in early-maturing EM. Furthermore, allelic polymorphism analysis of <i>AhIAA-1A</i> and <i>AhIAA-1B</i>, which were specifically expressed in seeds, showed that three SNP loci in 3′UTR of <i>AhIAA-1A</i> could effectively distinguish the EM- and LM- type germplasm, providing a basis for breeding markers development. Our results offered a comprehensive understanding of <i>Aux/IAA</i> genes in peanut and provided valuable clues for further investigation of the auxin signal transduction pathway and auxin regulation mechanism in peanut.https://www.mdpi.com/2223-7747/11/4/472auxin<i>Aux/IAA</i>peanutseed developmentgrowth period |
| spellingShingle | Xiurong Zhang Kun Zhang Lu Luo Yuying Lv Yuying Li Suqing Zhu Bing Luo Yongshan Wan Xiansheng Zhang Fengzhen Liu Identification of Peanut <i>Aux/IAA</i> Genes and Functional Prediction during Seed Development and Maturation Plants auxin <i>Aux/IAA</i> peanut seed development growth period |
| title | Identification of Peanut <i>Aux/IAA</i> Genes and Functional Prediction during Seed Development and Maturation |
| title_full | Identification of Peanut <i>Aux/IAA</i> Genes and Functional Prediction during Seed Development and Maturation |
| title_fullStr | Identification of Peanut <i>Aux/IAA</i> Genes and Functional Prediction during Seed Development and Maturation |
| title_full_unstemmed | Identification of Peanut <i>Aux/IAA</i> Genes and Functional Prediction during Seed Development and Maturation |
| title_short | Identification of Peanut <i>Aux/IAA</i> Genes and Functional Prediction during Seed Development and Maturation |
| title_sort | identification of peanut i aux iaa i genes and functional prediction during seed development and maturation |
| topic | auxin <i>Aux/IAA</i> peanut seed development growth period |
| url | https://www.mdpi.com/2223-7747/11/4/472 |
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