Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plant
Background: Litchi (Litchi chinensis) is an important sub-tropical fruit in the horticulture market in China. Breeding for improved fruit characteristics is needed for satisfying consumer demands. Budding is a sustainable method for its propagation. During our ongoing breeding program, we observed a...
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1360138/full |
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| author | Ning Xu Xian-quan Qin Dong-bo Li Yan-jie Hou Chen Fang Shu-wei Zhang Jing-yi You Hong-Li Li Hong-ye Qiu |
| author_facet | Ning Xu Xian-quan Qin Dong-bo Li Yan-jie Hou Chen Fang Shu-wei Zhang Jing-yi You Hong-Li Li Hong-ye Qiu |
| author_sort | Ning Xu |
| collection | DOAJ |
| description | Background: Litchi (Litchi chinensis) is an important sub-tropical fruit in the horticulture market in China. Breeding for improved fruit characteristics is needed for satisfying consumer demands. Budding is a sustainable method for its propagation. During our ongoing breeding program, we observed a litchi mutant with flat leaves and sharp fruit peel cracking in comparison to the curled leaves and blunt fruit peel cracking fruits of the mother plant.Methods: To understand the possible molecular pathways involved, we performed a combined metabolome and transcriptome analysis.Results: We identified 1,060 metabolites in litchi leaves and fruits, of which 106 and 101 were differentially accumulated between the leaves and fruits, respectively. The mutant leaves were richer in carbohydrates, nucleotides, and phenolic acids, while the mother plant was rich in most of the amino acids and derivatives, flavonoids, lipids and organic acids and derivatives, and vitamins. Contrastingly, mutant fruits had higher levels of amino acids and derivatives, carbohydrates and derivatives, and organic acids and derivatives. However, the mother plant’s fruits contained higher levels of flavonoids, scopoletin, amines, some amino acids and derivatives, benzamidine, carbohydrates and derivatives, and some organic acids and derivatives. The number of differentially expressed genes was consistent with the metabolome profiles. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway-enriched gene expressions showed consistent profiles as of metabolome analysis.Conclusion: These results provide the groundwork for breeding litchi for fruit and leaf traits that are useful for its taste and yield. |
| first_indexed | 2024-03-07T22:56:47Z |
| format | Article |
| id | doaj.art-f5d5d7724cc0472eb589e07eeef873ab |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-07T22:56:47Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-f5d5d7724cc0472eb589e07eeef873ab2024-02-23T04:22:04ZengFrontiers Media S.A.Frontiers in Genetics1664-80212024-02-011510.3389/fgene.2024.13601381360138Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plantNing XuXian-quan QinDong-bo LiYan-jie HouChen FangShu-wei ZhangJing-yi YouHong-Li LiHong-ye QiuBackground: Litchi (Litchi chinensis) is an important sub-tropical fruit in the horticulture market in China. Breeding for improved fruit characteristics is needed for satisfying consumer demands. Budding is a sustainable method for its propagation. During our ongoing breeding program, we observed a litchi mutant with flat leaves and sharp fruit peel cracking in comparison to the curled leaves and blunt fruit peel cracking fruits of the mother plant.Methods: To understand the possible molecular pathways involved, we performed a combined metabolome and transcriptome analysis.Results: We identified 1,060 metabolites in litchi leaves and fruits, of which 106 and 101 were differentially accumulated between the leaves and fruits, respectively. The mutant leaves were richer in carbohydrates, nucleotides, and phenolic acids, while the mother plant was rich in most of the amino acids and derivatives, flavonoids, lipids and organic acids and derivatives, and vitamins. Contrastingly, mutant fruits had higher levels of amino acids and derivatives, carbohydrates and derivatives, and organic acids and derivatives. However, the mother plant’s fruits contained higher levels of flavonoids, scopoletin, amines, some amino acids and derivatives, benzamidine, carbohydrates and derivatives, and some organic acids and derivatives. The number of differentially expressed genes was consistent with the metabolome profiles. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway-enriched gene expressions showed consistent profiles as of metabolome analysis.Conclusion: These results provide the groundwork for breeding litchi for fruit and leaf traits that are useful for its taste and yield.https://www.frontiersin.org/articles/10.3389/fgene.2024.1360138/fullamino acids and derivativescarbohydrates and derivativesflavonoid metabolome in fruitsleaf foldinglitchi fruit |
| spellingShingle | Ning Xu Xian-quan Qin Dong-bo Li Yan-jie Hou Chen Fang Shu-wei Zhang Jing-yi You Hong-Li Li Hong-ye Qiu Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plant Frontiers in Genetics amino acids and derivatives carbohydrates and derivatives flavonoid metabolome in fruits leaf folding litchi fruit |
| title | Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plant |
| title_full | Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plant |
| title_fullStr | Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plant |
| title_full_unstemmed | Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plant |
| title_short | Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plant |
| title_sort | comparative transcriptome and metabolome profiles of the leaf and fruits of a xianjinfeng litchi budding mutant and its mother plant |
| topic | amino acids and derivatives carbohydrates and derivatives flavonoid metabolome in fruits leaf folding litchi fruit |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1360138/full |
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