RETRACTED: Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumors
BackgroundIn children, Wilms’ tumors are the most common urological cancer with unsatisfactory prognosis, but few molecular prognostic markers have been discovered for it. With the rapid development of high-throughput quantitative proteomic and transcriptomic approaches, the molecular mechanisms of...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.982110/full |
| _version_ | 1797257224620343296 |
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| author | Lei Lou Lei Lou Luping Chen Luping Chen Yaohao Wu Gang Zhang Ronglin Qiu Jianhang Su Zhuangjie Zhao Zijie Lu Minyi Liao Xiaogeng Deng |
| author_facet | Lei Lou Lei Lou Luping Chen Luping Chen Yaohao Wu Gang Zhang Ronglin Qiu Jianhang Su Zhuangjie Zhao Zijie Lu Minyi Liao Xiaogeng Deng |
| author_sort | Lei Lou |
| collection | DOAJ |
| description | BackgroundIn children, Wilms’ tumors are the most common urological cancer with unsatisfactory prognosis, but few molecular prognostic markers have been discovered for it. With the rapid development of high-throughput quantitative proteomic and transcriptomic approaches, the molecular mechanisms of various cancers have been comprehensively explored. This study aimed to uncover the molecular mechanisms underlying Wilms tumor and build predictive models by use of microarray and RNA-seq data.MethodsGene expression datasets were downloaded from Therapeutically Applicable Research to Generate Effective Treatments (TARGET) and Gene Expression Omnibus (GEO) databases. Bioinformatics methods wereutilized to identified hub genes, and these hub genes were validated by experiment. Nomogram predicting OS was developed using genetic risk score model and clinicopathological variables.ResultsCDC20, BUB1 and CCNB2 were highly expressed in tumor tissues and able to affect cell proliferation and the cell cycle of SK-NEP-1 cells. This may reveal molecular biology features and a new therapeutic target of Wilms tumour.7 genes were selected as prognostic genes after univariate, Lasso, and multivariate Cox regression analyses and had good accuracy, a prognostic nomogram combined gene model with clinical factors was completed with high accuracy.ConclusionsThe current study discovered CDC20,BUB1 and CCNB2 as hub-genes associated with Wilms tumor, providing references to understand the pathogenesis and be considered a novel candidate to target therapy and construct novel nomogram, incorporating both clinical risk factors and gene model, could be appropriately applied in preoperative individualized prediction of malignancy in patients with Wilms tumor. |
| first_indexed | 2024-03-11T17:56:37Z |
| format | Article |
| id | doaj.art-f5d6835d90b04d4a9b4e74b862cddd1a |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-24T22:34:15Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-f5d6835d90b04d4a9b4e74b862cddd1a2024-03-19T13:57:09ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-10-011210.3389/fonc.2022.982110982110RETRACTED: Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumorsLei Lou0Lei Lou1Luping Chen2Luping Chen3Yaohao Wu4Gang Zhang5Ronglin Qiu6Jianhang Su7Zhuangjie Zhao8Zijie Lu9Minyi Liao10Xiaogeng Deng11Department of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Pediatric Surgery, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Pediatric Surgery, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaDepartment of Pediatric Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, ChinaBackgroundIn children, Wilms’ tumors are the most common urological cancer with unsatisfactory prognosis, but few molecular prognostic markers have been discovered for it. With the rapid development of high-throughput quantitative proteomic and transcriptomic approaches, the molecular mechanisms of various cancers have been comprehensively explored. This study aimed to uncover the molecular mechanisms underlying Wilms tumor and build predictive models by use of microarray and RNA-seq data.MethodsGene expression datasets were downloaded from Therapeutically Applicable Research to Generate Effective Treatments (TARGET) and Gene Expression Omnibus (GEO) databases. Bioinformatics methods wereutilized to identified hub genes, and these hub genes were validated by experiment. Nomogram predicting OS was developed using genetic risk score model and clinicopathological variables.ResultsCDC20, BUB1 and CCNB2 were highly expressed in tumor tissues and able to affect cell proliferation and the cell cycle of SK-NEP-1 cells. This may reveal molecular biology features and a new therapeutic target of Wilms tumour.7 genes were selected as prognostic genes after univariate, Lasso, and multivariate Cox regression analyses and had good accuracy, a prognostic nomogram combined gene model with clinical factors was completed with high accuracy.ConclusionsThe current study discovered CDC20,BUB1 and CCNB2 as hub-genes associated with Wilms tumor, providing references to understand the pathogenesis and be considered a novel candidate to target therapy and construct novel nomogram, incorporating both clinical risk factors and gene model, could be appropriately applied in preoperative individualized prediction of malignancy in patients with Wilms tumor.https://www.frontiersin.org/articles/10.3389/fonc.2022.982110/fullwilms tumorhub generisk stratificationprognostic modelnomogram |
| spellingShingle | Lei Lou Lei Lou Luping Chen Luping Chen Yaohao Wu Gang Zhang Ronglin Qiu Jianhang Su Zhuangjie Zhao Zijie Lu Minyi Liao Xiaogeng Deng RETRACTED: Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumors Frontiers in Oncology wilms tumor hub gene risk stratification prognostic model nomogram |
| title | RETRACTED: Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumors |
| title_full | RETRACTED: Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumors |
| title_fullStr | RETRACTED: Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumors |
| title_full_unstemmed | RETRACTED: Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumors |
| title_short | RETRACTED: Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumors |
| title_sort | retracted identification of hub genes and construction of prognostic nomogram for patients with wilms tumors |
| topic | wilms tumor hub gene risk stratification prognostic model nomogram |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.982110/full |
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