Molecular testing devices for on-site detection of E. coli in water samples
Abstract Escherichia coli (E. coli) cells are present in fecal materials that can be the main source for disease‐causing agents in water. As a result, E. coli is recommended as a water quality indicator. We have developed an innovative platform to detect E. coli for monitoring water quality on-site...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31208-4 |
| _version_ | 1797865163585486848 |
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| author | Carlos Manzanas Elise Morrison Young S. Kim Morteza Alipanah George Adedokun Shouguang Jin Todd Z. Osborne Z. Hugh Fan |
| author_facet | Carlos Manzanas Elise Morrison Young S. Kim Morteza Alipanah George Adedokun Shouguang Jin Todd Z. Osborne Z. Hugh Fan |
| author_sort | Carlos Manzanas |
| collection | DOAJ |
| description | Abstract Escherichia coli (E. coli) cells are present in fecal materials that can be the main source for disease‐causing agents in water. As a result, E. coli is recommended as a water quality indicator. We have developed an innovative platform to detect E. coli for monitoring water quality on-site by integrating paper-based sample preparation with nucleic acid isothermal amplification. The platform carries out bacterial lysis and DNA enrichment onto a paper pad through ball-based valves for fluid control, with no need of laboratory equipment, followed by loop-mediated isothermal amplification (LAMP) in a battery-operated coffee mug, and colorimetric detection. We have used the platform to detect E. coli in environmental water samples in about 1 h, with a limit of quantitation of 0.2 CFU/mL, and 3 copies per reaction. The platform was confirmed for detecting multiple E. coli strains, and for water samples of different salt concentrations. We validated the functions of the platform by analyzing recreational water samples collected near the Atlantic Ocean that contain different concentrations of salt and bacteria. |
| first_indexed | 2024-04-09T23:03:30Z |
| format | Article |
| id | doaj.art-f5db7505236c43f2942232491f277b4a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T23:03:30Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f5db7505236c43f2942232491f277b4a2023-03-22T10:52:18ZengNature PortfolioScientific Reports2045-23222023-03-0113111110.1038/s41598-023-31208-4Molecular testing devices for on-site detection of E. coli in water samplesCarlos Manzanas0Elise Morrison1Young S. Kim2Morteza Alipanah3George Adedokun4Shouguang Jin5Todd Z. Osborne6Z. Hugh Fan7Interdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of FloridaDepartment of Environmental Engineering Sciences, University of FloridaDepartment of Molecular Genetics and Microbiology, University of FloridaInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of FloridaInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of FloridaDepartment of Molecular Genetics and Microbiology, University of FloridaWhitney Laboratory of Marine Bioscience, University of FloridaInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of FloridaAbstract Escherichia coli (E. coli) cells are present in fecal materials that can be the main source for disease‐causing agents in water. As a result, E. coli is recommended as a water quality indicator. We have developed an innovative platform to detect E. coli for monitoring water quality on-site by integrating paper-based sample preparation with nucleic acid isothermal amplification. The platform carries out bacterial lysis and DNA enrichment onto a paper pad through ball-based valves for fluid control, with no need of laboratory equipment, followed by loop-mediated isothermal amplification (LAMP) in a battery-operated coffee mug, and colorimetric detection. We have used the platform to detect E. coli in environmental water samples in about 1 h, with a limit of quantitation of 0.2 CFU/mL, and 3 copies per reaction. The platform was confirmed for detecting multiple E. coli strains, and for water samples of different salt concentrations. We validated the functions of the platform by analyzing recreational water samples collected near the Atlantic Ocean that contain different concentrations of salt and bacteria.https://doi.org/10.1038/s41598-023-31208-4 |
| spellingShingle | Carlos Manzanas Elise Morrison Young S. Kim Morteza Alipanah George Adedokun Shouguang Jin Todd Z. Osborne Z. Hugh Fan Molecular testing devices for on-site detection of E. coli in water samples Scientific Reports |
| title | Molecular testing devices for on-site detection of E. coli in water samples |
| title_full | Molecular testing devices for on-site detection of E. coli in water samples |
| title_fullStr | Molecular testing devices for on-site detection of E. coli in water samples |
| title_full_unstemmed | Molecular testing devices for on-site detection of E. coli in water samples |
| title_short | Molecular testing devices for on-site detection of E. coli in water samples |
| title_sort | molecular testing devices for on site detection of e coli in water samples |
| url | https://doi.org/10.1038/s41598-023-31208-4 |
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