Functional Analysis of a Putative Target of Spatially Varying Selection in the Menin1 Gene of Drosophila melanogaster
While significant effort has been devoted to investigating the potential influence of spatially varying selection on genomic variation, relatively little effort has been devoted to experimental analysis of putative variants or genes experiencing such selection. Previous population genetic work ident...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2019-01-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.118.200818 |
| _version_ | 1818890009234636800 |
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| author | Nicolas Svetec Perot Saelao Julie M. Cridland Ary A. Hoffmann David J. Begun |
| author_facet | Nicolas Svetec Perot Saelao Julie M. Cridland Ary A. Hoffmann David J. Begun |
| author_sort | Nicolas Svetec |
| collection | DOAJ |
| description | While significant effort has been devoted to investigating the potential influence of spatially varying selection on genomic variation, relatively little effort has been devoted to experimental analysis of putative variants or genes experiencing such selection. Previous population genetic work identified an amino acid polymorphism in the Mnn1 gene as one of the most strongly latitudinally differentiated SNPs in the genome of Drosophila melanogaster in the United States and Australia. Here we report the results of our transgenic analysis of this amino acid polymorphism. Genotypes carrying alternative Mnn1 alleles differed in multiple phenotypes in a direction generally consistent with phenotypic differences previously observed along latitudinal clines. These results support inferences from earlier population genomic work that this variant influences fitness, and support the idea that the alleles exhibiting clines may be likely to have pleiotropic effects that are correlated along the axes favored by natural selection. |
| first_indexed | 2024-12-19T17:18:06Z |
| format | Article |
| id | doaj.art-f5dbf954590343fa827207697b2e06c6 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-19T17:18:06Z |
| publishDate | 2019-01-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-f5dbf954590343fa827207697b2e06c62022-12-21T20:12:49ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362019-01-0191738010.1534/g3.118.2008187Functional Analysis of a Putative Target of Spatially Varying Selection in the Menin1 Gene of Drosophila melanogasterNicolas SvetecPerot SaelaoJulie M. CridlandAry A. HoffmannDavid J. BegunWhile significant effort has been devoted to investigating the potential influence of spatially varying selection on genomic variation, relatively little effort has been devoted to experimental analysis of putative variants or genes experiencing such selection. Previous population genetic work identified an amino acid polymorphism in the Mnn1 gene as one of the most strongly latitudinally differentiated SNPs in the genome of Drosophila melanogaster in the United States and Australia. Here we report the results of our transgenic analysis of this amino acid polymorphism. Genotypes carrying alternative Mnn1 alleles differed in multiple phenotypes in a direction generally consistent with phenotypic differences previously observed along latitudinal clines. These results support inferences from earlier population genomic work that this variant influences fitness, and support the idea that the alleles exhibiting clines may be likely to have pleiotropic effects that are correlated along the axes favored by natural selection.http://g3journal.org/lookup/doi/10.1534/g3.118.200818DNA damagechill coma recoverylocal adaptationlatitudinal cline |
| spellingShingle | Nicolas Svetec Perot Saelao Julie M. Cridland Ary A. Hoffmann David J. Begun Functional Analysis of a Putative Target of Spatially Varying Selection in the Menin1 Gene of Drosophila melanogaster G3: Genes, Genomes, Genetics DNA damage chill coma recovery local adaptation latitudinal cline |
| title | Functional Analysis of a Putative Target of Spatially Varying Selection in the Menin1 Gene of Drosophila melanogaster |
| title_full | Functional Analysis of a Putative Target of Spatially Varying Selection in the Menin1 Gene of Drosophila melanogaster |
| title_fullStr | Functional Analysis of a Putative Target of Spatially Varying Selection in the Menin1 Gene of Drosophila melanogaster |
| title_full_unstemmed | Functional Analysis of a Putative Target of Spatially Varying Selection in the Menin1 Gene of Drosophila melanogaster |
| title_short | Functional Analysis of a Putative Target of Spatially Varying Selection in the Menin1 Gene of Drosophila melanogaster |
| title_sort | functional analysis of a putative target of spatially varying selection in the menin1 gene of drosophila melanogaster |
| topic | DNA damage chill coma recovery local adaptation latitudinal cline |
| url | http://g3journal.org/lookup/doi/10.1534/g3.118.200818 |
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