Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection
The IGRA (Interferon Gamma Release Assays) test is currently the standard specific test for <i>Mycobacterium tuberculosis</i> infection status. However, a positive test cannot distinguish between active tuberculosis disease (ATBD) and latent tuberculosis infection (LTBI). Developing a te...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1385 |
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| author | Cesar Sanchez Luis Jaramillo-Valverde Silvia Capristano Gilmer Solis Alonso Soto Julio Valdivia-Silva Julio A. Poterico Heinner Guio |
| author_facet | Cesar Sanchez Luis Jaramillo-Valverde Silvia Capristano Gilmer Solis Alonso Soto Julio Valdivia-Silva Julio A. Poterico Heinner Guio |
| author_sort | Cesar Sanchez |
| collection | DOAJ |
| description | The IGRA (Interferon Gamma Release Assays) test is currently the standard specific test for <i>Mycobacterium tuberculosis</i> infection status. However, a positive test cannot distinguish between active tuberculosis disease (ATBD) and latent tuberculosis infection (LTBI). Developing a test with this characteristic is needed. We conducted longitudinal studies to identify a combination of antigen peptides and cytokines to discriminate between ATBD and LTBI. We studied 54 patients with ATBD disease and 51 with LTBI infection. Cell culture supernatant from cells stimulated with overlapping <i>Mycobacterium tuberculosis</i> novel peptides and 40 cytokines/chemokines were analyzed using the Luminex technology. To summarize longitudinal measurements of analyte levels, we calculated the area under the curve (AUC). Our results indicate that in vitro cell stimulation with a novel combination of peptides (Rv0849-12, Rv2031c-14, Rv2031c-5, and Rv2693-06) and IL-1RA detection in culture supernatants can discriminate between LTBI and ATBD. |
| first_indexed | 2024-03-11T02:08:49Z |
| format | Article |
| id | doaj.art-f5dc2fa6f63443ec9e21f49085efdecf |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:49Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f5dc2fa6f63443ec9e21f49085efdecf2023-11-18T11:41:10ZengMDPI AGMicroorganisms2076-26072023-05-01116138510.3390/microorganisms11061385Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis InfectionCesar Sanchez0Luis Jaramillo-Valverde1Silvia Capristano2Gilmer Solis3Alonso Soto4Julio Valdivia-Silva5Julio A. Poterico6Heinner Guio7Laboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima 15046, PeruLaboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima 15046, PeruLaboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima 15046, PeruLaboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima 15046, PeruHospital Nacional Hipólito Unanue, Lima 15007, PeruINBIOMEDIC Research and Technological Center, Lima 15046, PeruINBIOMEDIC Research and Technological Center, Lima 15046, PeruLaboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima 15046, PeruThe IGRA (Interferon Gamma Release Assays) test is currently the standard specific test for <i>Mycobacterium tuberculosis</i> infection status. However, a positive test cannot distinguish between active tuberculosis disease (ATBD) and latent tuberculosis infection (LTBI). Developing a test with this characteristic is needed. We conducted longitudinal studies to identify a combination of antigen peptides and cytokines to discriminate between ATBD and LTBI. We studied 54 patients with ATBD disease and 51 with LTBI infection. Cell culture supernatant from cells stimulated with overlapping <i>Mycobacterium tuberculosis</i> novel peptides and 40 cytokines/chemokines were analyzed using the Luminex technology. To summarize longitudinal measurements of analyte levels, we calculated the area under the curve (AUC). Our results indicate that in vitro cell stimulation with a novel combination of peptides (Rv0849-12, Rv2031c-14, Rv2031c-5, and Rv2693-06) and IL-1RA detection in culture supernatants can discriminate between LTBI and ATBD.https://www.mdpi.com/2076-2607/11/6/1385<i>Mycobacterium tuberculosis</i>biomarkersactive tuberculosislatent tuberculosisimmune responseIL-1RA |
| spellingShingle | Cesar Sanchez Luis Jaramillo-Valverde Silvia Capristano Gilmer Solis Alonso Soto Julio Valdivia-Silva Julio A. Poterico Heinner Guio Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection Microorganisms <i>Mycobacterium tuberculosis</i> biomarkers active tuberculosis latent tuberculosis immune response IL-1RA |
| title | Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection |
| title_full | Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection |
| title_fullStr | Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection |
| title_full_unstemmed | Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection |
| title_short | Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection |
| title_sort | antigen induced il 1ra production discriminates active and latent tuberculosis infection |
| topic | <i>Mycobacterium tuberculosis</i> biomarkers active tuberculosis latent tuberculosis immune response IL-1RA |
| url | https://www.mdpi.com/2076-2607/11/6/1385 |
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